Protocols shared by authors via RaP service
Journal
Repeat pseudo-genome preparation
Author: Mohamed Nadhir Djekidel
Date: Jan 23, 2021，Views：1106
Original protocol used in research article：
A transcriptional roadmap for 2C-like–to–pluripotent state transition
Science Advances, May 28, 2020; DOI: 10.1126/sciadv.aay5181
Immunohistochemistry and imaging
Author: Mehak M Khan
Date: Jan 23, 2021，Views：860
Original protocol used in research article：
Loss of Doc2b does not influence transmission at Purkinje cell to deep nuclei synapses under physiological conditions
eLife, Apr 29, 2020; DOI: 10.7554/eLife.55165
Expression variability estimation
Author: Benjamin Jung Fair
Date: Jan 22, 2021，Views：1010
Original protocol used in research article：
Gene expression variability in human and chimpanzee populations share common determinants
eLife, Oct 21, 2020; DOI: 10.7554/eLife.59929
In vitro cellular experiments
Author: Jingjing Liang
Date: Jan 19, 2021，Views：899
Original protocol used in research article：
Nanoparticle-enhanced chemo-immunotherapy to trigger robust antitumor immunity
Science Advances, Aug 27, 2020; DOI: 10.1126/sciadv.abc3646
Microgel fabrication and tethering of SA-PD-L1
Authors: María M. Coronel, Haval Shirwan and Andrés J. García
Date: Jan 19, 2021，Views：964
Original protocol used in research article：
Immunotherapy via PD-L1–presenting biomaterials leads to long-term islet graft survival
Science Advances, Aug 27, 2020; DOI: 10.1126/sciadv.aba5573
Nano-LC-MS/MS analysis
Author: Wenqing Shui
Date: Jan 19, 2021，Views：916
Original protocol used in research article：
Quantitative proteomics reveal proteins enriched in tubular endoplasmic reticulum of Saccharomyces cerevisiae
eLife, Mar 13, 2017; DOI: 10.7554/eLife.23816
Yeast microsome preparation
Author: Wenqing Shui
Date: Jan 19, 2021，Views：1930
Original protocol used in research article：
Quantitative proteomics reveal proteins enriched in tubular endoplasmic reticulum of Saccharomyces cerevisiae
eLife, Mar 13, 2017; DOI: 10.7554/eLife.23816
Collection of in vivo capacitated sperm
Authors: Lukas Ded and Jean-Ju Chung
Date: Jan 19, 2021，Views：1224
Original protocol used in research article：
3D in situ imaging of the female reproductive tract reveals molecular signatures of fertilizing spermatozoa in mice
eLife, Oct 20, 2020; DOI: 10.7554/eLife.62043
Flow cytometry analysis
Author: Hong-Ru Chen
Date: Jan 19, 2021，Views：877
Original protocol used in research article：
Fate mapping via CCR2-CreER mice reveals monocyte-to-microglia transition in development and neonatal stroke
Science Advances, Aug 25, 2020; DOI: 10.1126/sciadv.abb2119
Immunohistochemistry
Author: Hong-Ru Chen
Date: Jan 19, 2021，Views：866
Original protocol used in research article：
Fate mapping via CCR2-CreER mice reveals monocyte-to-microglia transition in development and neonatal stroke
Science Advances, Aug 25, 2020; DOI: 10.1126/sciadv.abb2119
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