Protocols shared by authors via RaP service
Journal
mRNA synthesis and formulation
Author: John L. Joyal
Date: Jan 26, 2021，Views：1240
Original protocol used in research article：
Impact of mRNA chemistry and manufacturing process on innate immune activation
Science Advances, Jun 23, 2020; DOI: 10.1126/sciadv.aaz6893
CAT filter assay
Author: Aurélien Olichon
Date: Jan 26, 2021，Views：999
Original protocol used in research article：
NaLi-H1: A universal synthetic library of humanized nanobodies providing highly functional antibodies and intrabodies
eLife, Jul 19, 2016; DOI: 10.7554/eLife.16228
Combined phosphoflow with RNA flow cytometry
Authors: Claire Ma, John C Marioni, Arianne C Richard and Gillian M Griffiths
Date: Jan 25, 2021，Views：1058
Original protocol used in research article：
Stimulation strength controls the rate of initiation but not the molecular organisation of TCR-induced signalling
eLife, May 15, 2020; DOI: 10.7554/eLife.53948
Whole-mount in situ hybridization of Hydra
Authors: Hiroyuki J. Kanaya, Yoshitaka Kobayakawa and Taichi Q. Itoh
Date: Jan 24, 2021，Views：978
Original protocol used in research article：
A sleep-like state in Hydra unravels conserved sleep mechanisms during the evolutionary development of the central nervous system
Science Advances, Oct 6, 2020; DOI: 10.1126/sciadv.abb9415
Differential gene expression analysis of bulk RNA-seq data
Author: Mohamed Nadhir Djekidel
Date: Jan 24, 2021，Views：994
Original protocol used in research article：
A transcriptional roadmap for 2C-like–to–pluripotent state transition
Science Advances, May 28, 2020; DOI: 10.1126/sciadv.aay5181
DNA library preparation
Authors: Ryan Muller and Nicholas T. Ingolia
Date: Jan 24, 2021，Views：1082
Original protocol used in research article：
CiBER-seq dissects genetic networks by quantitative CRISPRi profiling of expression phenotypes
Science, Dec 11, 2020; DOI: 10.1126/science.abb9662
Plasmid library construction
Authors: Ryan Muller and Nicholas T Ingolia
Date: Jan 24, 2021，Views：1224
Original protocol used in research article：
CiBER-seq dissects genetic networks by quantitative CRISPRi profiling of expression phenotypes
Science, Dec 11, 2020; DOI: 10.1126/science.abb9662
Genomic annotation files preparation
Author: Mohamed Nadhir Djekidel
Date: Jan 24, 2021，Views：1435
Original protocol used in research article：
A transcriptional roadmap for 2C-like–to–pluripotent state transition
Science Advances, May 28, 2020; DOI: 10.1126/sciadv.aay5181
Extracorporeal circulation treatment
Author: He-Xin Yan
Date: Jan 24, 2021，Views：762
Original protocol used in research article：
An extracorporeal bioartificial liver embedded with 3D-layered human liver progenitor-like cells relieves acute liver failure in pigs
Science Translational Medicine, Jul 7, 2020; DOI: 10.1126/scitranslmed.aba5146
Cell culture and hepatocytes optimization
Author: Weijian Li
Date: Jan 24, 2021，Views：806
Original protocol used in research article：
An extracorporeal bioartificial liver embedded with 3D-layered human liver progenitor-like cells relieves acute liver failure in pigs
Science Translational Medicine, Jul 7, 2020; DOI: 10.1126/scitranslmed.aba5146
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