Readers should cite both the Bio-protocol preprint and the original research article where this protocol was used:

Yu, Z(2021). Sequences, plasmids, transfection and RNA interference. Bio-protocol Preprint. bio-protocol.org/prep866.
Yu, Z., Chen, T., Li, X., Yang, M., Tang, S., Zhu, X., Gu, Y., Su, X., Xia, M., Li, W., Zhang, X., Wang, Q., Cao, X. and Wang, J.(2016). Lys29-linkage of ASK1 by Skp1−Cullin 1−Fbxo21 ubiquitin ligase complex is required for antiviral innate response. eLIFE. DOI: 10.7554/eLife.14087