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Preprint

CRISPR library construction and screening

RL Rui Lu
CL Chunliang Li

Last updated date: Mar 1, 2021 Views: 1150 Forks: 0

original An abbreviated version of this protocol was published in eLife in Oct, 2020
Functional interrogation of HOXA9 regulome in MLLr leukemia via reporter-based CRISPR/Cas9 screen
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Enclosed please find the protocol for TF CRISPR screen. We have also deposited the library plasmid and a detailed version of protocol to Addgene (#162275). 

Related files

zip addgene TF library deposit.zip download
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How to cite：
Readers should cite both the Bio-protocol preprint and the original research article where this protocol was used:
  1. Lu, R and Li, C(2021). CRISPR library construction and screening. Bio-protocol Preprint. bio-protocol.org/prep888.
  2. Zhang, H., Zhang, Y., Zhou, X., Wright, S., Hyle, J., Zhao, L., An, J., Zhao, X., Shao, Y., Xu, B., Lee, H., Chen, T., Zhou, Y., Chen, X., Lu, R. and Li, C.(2020). Functional interrogation of HOXA9 regulome in MLLr leukemia via reporter-based CRISPR/Cas9 screen. eLife. DOI: 10.7554/eLife.57858

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