Protocols shared by authors via RaP service
Journal
RNA-seq and differential expression power analysis
Author: Benjamin Jung Fair
Date: Apr 6, 2021，Views：976
Original protocol used in research article：
Gene expression variability in human and chimpanzee populations share common determinants
eLife, Oct 21, 2020; DOI: 10.7554/eLife.59929
Confocal microscopy, live cell imaging, and intravital microscopy
Authors: Sang Hun Lee and David L. Sacks
Date: Apr 5, 2021，Views：914
Original protocol used in research article：
M2-like dermal macrophages are maintained via IL-4/CCL24–mediated cooperative interaction with eosinophils in cutaneous leishmaniasis
Science Immunology, Apr 9, 2020; DOI: 10.1126/sciimmunol.aaz4415
Oviductal sperm counting
Authors: Wipawee Winuthayanon and Carmen J Williams
Date: Apr 2, 2021，Views：788
Original protocol used in research article：
Oviductal estrogen receptor α signaling prevents protease-mediated embryo death
eLife, Dec 1, 2015; DOI: 10.7554/eLife.10453
Immunofluorescence
Authors: Shang Yin Chiang, Ruey-Hwa Chen and Shen-Ju Chou
Date: Apr 2, 2021，Views：830
Original protocol used in research article：
Usp11 controls cortical neurogenesis and neuronal migration through Sox11 stabilization
Science Advances, Feb 11, 2021; DOI: 10.1126/sciadv.abc6093
Cell staining and imaging
Author: Anne E Carpenter
Date: Apr 2, 2021，Views：920
Original protocol used in research article：
Systematic morphological profiling of human gene and allele function via Cell Painting
eLife, Mar 18, 2017; DOI: 10.7554/eLife.24060
NMR measurements of phase-separating samples
Authors: Rob W. H. Ruigrok and Martin Blackledge
Date: Apr 2, 2021，Views：859
Original protocol used in research article：
Measles virus nucleo- and phosphoproteins form liquid-like phase-separated compartments that promote nucleocapsid assembly
Science Advances, Apr 1, 2020; DOI: 10.1126/sciadv.aaz7095
Calculation of dice scores
Authors: Yunyun Han and Peng Fei
Date: Apr 1, 2021，Views：827
Original protocol used in research article：
Bi-channel image registration and deep-learning segmentation (BIRDS) for efficient, versatile 3D mapping of mouse brain
eLife, Jan 18, 2021; DOI: 10.7554/eLife.63455
Differentiation of human and mouse THGM cells in vitro
Authors: Javad Rasouli, Bogoljub Ciric and Abdolmohamad Rostami
Date: Apr 1, 2021，Views：916
Original protocol used in research article：
A distinct GM-CSF+ T helper cell subset requires T-bet to adopt a TH1 phenotype and promote neuroinflammation
Science Immunology, Oct 22, 2020; DOI: 10.1126/sciimmunol.aba9953
PDC isolation and culture
Authors: Li-Wei Dong, Ye-Xiong Tan and Hong-Yang Wang
Date: Apr 1, 2021，Views：822
Original protocol used in research article：
PTEN status determines chemosensitivity to proteasome inhibition in cholangiocarcinoma
Science Translational Medicine, Sep 22, 2020; DOI: 10.1126/scitranslmed.aay0152
Activity recording
Authors: Yusaku Hontani, Fei Xia and Chris Xu
Date: Mar 31, 2021，Views：960
Original protocol used in research article：
Multicolor three-photon fluorescence imaging with single-wavelength excitation deep in mouse brain
Science Advances, Mar 17, 2021; DOI: 10.1126/sciadv.abf3531
Other Resources
www.bio-protocol.org
for advanced protocols
www.bio-thing.com
for reagents & equipment
lab.bio-thing.cn
for lab management
For Advertisers
Advertising Integrity
Board
Advertising Policy
Advertising Terms and
Conditions
For Authors
Submission Procedure
Author guidelines
Submit a Protocol
About
About Us

Become an Ambassador
©  Bio-protocol LLC. ISSN: 2331-8325 京ICP备11029730号-1 Terms of Service | Privacy Policy | Contact Us
Find out more
We use cookies to improve your user experience on this site. By using our website, you agree to the storage of cookies on your computer.