Protocols shared by authors via RaP service
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In vitro kinase assays for STPKs.
Author: Ramandeep Singh
Date: Jun 30, 2021，Views：878
Original protocol used in research article：
NU-6027 Inhibits Growth of Mycobacterium tuberculosis by Targeting Protein Kinase D and Protein Kinase G
Antimicrobial Agents and Chemotherapy, Aug 23, 2019; DOI: 10.1128/AAC.00996-19
2.5. Cell proliferation assay
Authors: Zhixiong Cai, Xiaolong Liu and Jingfeng Liu
Date: Jun 30, 2021，Views：1230
Original protocol used in research article：
Circular RNA profiling identifies circADAMTS13 as a miR‐484 sponge which suppresses cell proliferation in hepatocellular carcinoma
Molecular Oncology, Jan 9, 2019; DOI: 10.1002/1878-0261.12424
Mixed bone marrow and mixed T cell adoptive transfer
Authors: Shao-Cong Sun and xiaofei zhou
Date: Jun 28, 2021，Views：892
Original protocol used in research article：
The deubiquitinase Otub1 controls the activation of CD8 T cells and NK cells by regulating IL-15-mediated priming
Nat Immunol, Jun 10, 2019; DOI: 10.1038/s41590-019-0405-2
SFB colonization
Authors: Daria Esterházy and Daniel Mucida
Date: Jun 28, 2021，Views：962
Original protocol used in research article：
Compartmentalized gut lymph node drainage dictates adaptive immune responses
Nature, Apr 15, 2019; DOI: 10.1038/s41586-019-1125-3
Membrane protein detection
Authors: Shao-Cong Sun and Xiaofei Zhou
Date: Jun 28, 2021，Views：918
Original protocol used in research article：
The deubiquitinase Otub1 controls the activation of CD8 T cells and NK cells by regulating IL-15-mediated priming
Nat Immunol, Jun 10, 2019; DOI: 10.1038/s41590-019-0405-2
FluoroSpot Assay for ASCs
Authors: Renata Varnaitė and Sara Gredmark-Russ
Date: Jun 28, 2021，Views：961
Original protocol used in research article：
Expansion of SARS-CoV-2–Specific Antibody-Secreting Cells and Generation of Neutralizing Antibodies in Hospitalized COVID-19 Patients
J Immunol, Sep 2, 2020; DOI: 10.4049/jimmunol.2000717
In vitro Suppression Assay and Transmigration Assay
Authors: Ivan Koprivica and Ivana Stojanović
Date: Jun 28, 2021，Views：941
Original protocol used in research article：
Ethyl Pyruvate Stimulates Regulatory T Cells and Ameliorates Type 1 Diabetes Development in Mice
Frontiers in Immunology, Jan 10, 2019; DOI: 10.3389/fimmu.2018.03130
Ubiquitination assay
Authors: Qingchen Zhu and Yichuan Xiao
Date: Jun 28, 2021，Views：1081
Original protocol used in research article：
Modulation of M2 macrophage polarization by the crosstalk between Stat6 and Trim24
Nature Communications, Sep 25, 2019; DOI: 10.1038/s41467-019-12384-2
In vitro Infection of Neutrophils With Leishmania donovani Promastigotes
Authors: Mareike Ohms, Tamás Laskay and Sonja Möller
Date: Jun 27, 2021，Views：877
Original protocol used in research article：
An Attempt to Polarize Human Neutrophils Toward N1 and N2 Phenotypes in vitro
Frontiers in Immunology, Apr 28, 2020; DOI: 10.3389/fimmu.2020.00532
In vitro SIRT6 deacetylation assay
Authors: Minxian Qian and Baohua Liu
Date: Jun 27, 2021，Views：982
Original protocol used in research article：
Deacetylase-independent function of SIRT6 couples GATA4 transcription factor and epigenetic activation against cardiomyocyte apoptosis
Nucleic Acids Research, Apr 2, 2020; DOI: 10.1093/nar/gkaa214
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