Protocols shared by authors via RaP service
Journal
Animal husbandry and handling
Authors: Laura A Lavery and Huda Y Zoghbi
Date: May 4, 2021，Views：876
Original protocol used in research article：
Losing Dnmt3a dependent methylation in inhibitory neurons impairs neural function by a mechanism impacting Rett syndrome
eLife, Mar 11, 2020; DOI: 10.7554/eLife.52981
Integrative RNA-seq and methylation analysis
Authors: Alexander Trostle, Laura A Lavery, Ying-wooi Wan and Huda Y Zoghbi
Date: May 4, 2021，Views：863
Original protocol used in research article：
Losing Dnmt3a dependent methylation in inhibitory neurons impairs neural function by a mechanism impacting Rett syndrome
eLife, Mar 11, 2020; DOI: 10.7554/eLife.52981
Animal experiments
Authors: Hyungsoo Kim and Ze’ev A. Ronai
Date: May 3, 2021，Views：908
Original protocol used in research article：
PRMT5 control of cGAS/STING and NLRC5 pathways defines melanoma response to antitumor immunity
Science Translational Medicine, Jul 7, 2020; DOI: 10.1126/scitranslmed.aaz5683
Real-time place preference – RTPP
Authors: Bárbara Coimbra and Ana João Rodrigues
Date: Apr 29, 2021，Views：1037
Original protocol used in research article：
Impairments in laterodorsal tegmentum to VTA projections underlie glucocorticoid-triggered reward deficits
eLife, Aug 24, 2017; DOI: 10.7554/eLife.25843
Binding energy calculations by molecular mechanics–generalized Born surface area
Authors: D. Collias, R. T. Leenay, R. A. Slotkowski, Z. Zuo, J. Liu and C. L. Beisel
Date: Apr 29, 2021，Views：959
Original protocol used in research article：
A positive growth-based PAM screen identifies noncanonical motifs recognized by the S. pyogenes Cas9
Science Advances, Jul 14, 2020; DOI: 10.1126/sciadv.abb4054
Dual RNA-seq sample preparation
Authors: Gili Rosenberg and Roi Avraham
Date: Apr 29, 2021，Views：943
Original protocol used in research article：
Host succinate is an activation signal for Salmonella virulence during intracellular infection
Science, Jan 22, 2021; DOI: 10.1126/science.aba8026
Whisker and other hair trimming
Authors: Kyle S Severson and Daniel H O'Connor
Date: Apr 28, 2021，Views：873
Original protocol used in research article：
Coding of whisker motion across the mouse face
eLife, Feb 28, 2019; DOI: 10.7554/eLife.41535
mtDNA extraction from Fr8 EVs and qPCR
Author: Efrat Levy
Date: Apr 28, 2021，Views：922
Original protocol used in research article：
Mitovesicles are a novel population of extracellular vesicles of mitochondrial origin altered in Down syndrome
Science Advances, Feb 11, 2021; DOI: 10.1126/sciadv.abe5085
In-situ proximity ligation
Authors: Konrad Basler, Andreas E Moor, Claudio Cantù and Amaia Jauregi-Miguel
Date: Apr 28, 2021，Views：847
Original protocol used in research article：
TBX3 acts as tissue-specific component of the Wnt/β-catenin transcriptional complex
eLife, Aug 18, 2020; DOI: 10.7554/eLife.58123
Microfabrication of microfluidic molds for layer-specific microchannel networks
Author: Tyler R Ray
Date: Apr 27, 2021，Views：878
Original protocol used in research article：
Soft, skin-interfaced sweat stickers for cystic fibrosis diagnosis and management
Science Translational Medicine, Mar 31, 2021; DOI: 10.1126/scitranslmed.abd8109
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