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Preprint

Reagents and antibodies

HD Hongyu Deng

Last updated date: Jun 3, 2021 Views: 839 Forks: 0

original An abbreviated version of this protocol was published in Science Advances in Nov, 2020
PPM1G restricts innate immune signaling mediated by STING and MAVS and is hijacked by KSHV for immune evasion
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Actually, there is no “specific” method, we used this antibody according to the manufacturer’s protocol. The antibody p-IRF3 we used is shown in the picture below. Recommended antibody dilutions for western blotting is 1:1000-1:2000. Good luck!

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How to cite：
Readers should cite both the Bio-protocol preprint and the original research article where this protocol was used:
  1. Deng, H(2021). Reagents and antibodies. Bio-protocol Preprint. bio-protocol.org/prep1134.
  2. Yu, K., Tian, H. and Deng, H.(2020). PPM1G restricts innate immune signaling mediated by STING and MAVS and is hijacked by KSHV for immune evasion . Science Advances 6(47). DOI: 10.1126/sciadv.abd0276

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Immunology

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