Protocols shared by authors via RaP service
Journal
Immunofluorescence
Authors: Li-Yin Hung and De’Broski R. Herbert
Date: May 21, 2021，Views：953
Original protocol used in research article：
Cellular context of IL-33 expression dictates impact on anti-helminth immunity
Science Immunology, Nov 12, 2020; DOI: 10.1126/sciimmunol.abc6259
Preparation of yeast membranes
Authors: Sisi Lin, Subhajit Mukherjee, Juanjuan Li, Weiliang Hou, Chao Pan and Jinyao Liu
Date: May 21, 2021，Views：1034
Original protocol used in research article：
Mucosal immunity–mediated modulation of the gut microbiome by oral delivery of probiotics into Peyer’s patches
Science Advances, May 12, 2021; DOI: 10.1126/sciadv.abf0677
MCAO Stroke Model Protocol for Mouse
Authors: Luke R Lemmerman, Hallie N Harris, W. David Arnold, Natalia Higuita-Castro and Daniel Gallego-Perez
Date: May 21, 2021，Views：1479
Original protocol used in research article：
Nanotransfection-based vasculogenic cell reprogramming drives functional recovery in a mouse model of ischemic stroke
Science Advances, Mar 18, 2021; DOI: 10.1126/sciadv.abd4735
Cell lines
Authors: Zuodong Zhao, Zhuqiang Zhang and Bing Zhu
Date: May 20, 2021，Views：821
Original protocol used in research article：
Sustained TNF-α stimulation leads to transcriptional memory that greatly enhances signal sensitivity and robustness
eLife, Nov 6, 2020; DOI: 10.7554/eLife.61965
Intestinal organoid cultures
Authors: Zuri Sullivan and Ruslan Medzhitov
Date: May 18, 2021，Views：908
Original protocol used in research article：
γδ T cells regulate the intestinal response to nutrient sensing
Science, Mar 19, 2021; DOI: 10.1126/science.aba8310
Data filtering and genome assembly
Authors: Reuben W Nowell and Timothy G Barraclough
Date: May 18, 2021，Views：895
Original protocol used in research article：
Evolutionary dynamics of transposable elements in bdelloid rotifers
eLife, Feb 5, 2021; DOI: 10.7554/eLife.63194
Data
Authors: Li Wang and Ni Huang
Date: May 17, 2021，Views：970
Original protocol used in research article：
Spatial and temporal variations in global soil respiration and their relationships with climate and land cover
Science Advances, Oct 6, 2020; DOI: 10.1126/sciadv.abb8508
Parameter optimizations of the three machine learning algorithm models
Authors: Ni Huang and Li Wang
Date: May 17, 2021，Views：883
Original protocol used in research article：
Spatial and temporal variations in global soil respiration and their relationships with climate and land cover
Science Advances, Oct 6, 2020; DOI: 10.1126/sciadv.abb8508
Whole Mount in situ Hybridization (WISH) on mouse embryos
Authors: Matthew J Anderson and Mark Lewandoski
Date: May 17, 2021，Views：904
Original protocol used in research article：
Fgf4 maintains Hes7 levels critical for normal somite segmentation clock function
eLife, Nov 19, 2020; DOI: 10.7554/eLife.55608
Characterization of CAR-transduced T cell cytotoxic potential
Authors: Vanaja Konduri and William K. Decker
Date: May 17, 2021，Views：927
Original protocol used in research article：
A subset of cytotoxic effector memory T cells enhances CAR T cell efficacy in a model of pancreatic ductal adenocarcinoma
Science Translational Medicine, May 5, 2021; DOI: 10.1126/scitranslmed.abc3196
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