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Preprint

SILAC labeling and DNA pull-down experiments

RH Reuben S Harris
DK Dennis Kappei

Last updated date: Jun 23, 2021 Views: 886 Forks: 0

original An abbreviated version of this protocol was published in eLife in Sep, 2020
Characterization of the mechanism by which the RB/E2F pathway controls expression of the cancer genomic DNA deaminase APOBEC3B
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We appreciate your interest in our protocol and would like to refer to a published methods paper: https://pubmed.ncbi.nlm.nih.gov/23436359/

This methods publication from Dr. Michiel Vermeulen’s lab uses the same protocol as us. It based on joint work by Dr. Falk Butter, Dr. Dennis Kappei (coauthor here), and Dr. Michiel Vermeulen when they worked together in Dr. Matthias Mann’s lab: https://pubmed.ncbi.nlm.nih.gov/20134481/

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How to cite：
Readers should cite both the Bio-protocol preprint and the original research article where this protocol was used:
  1. Harris, R and Kappei, D(2021). SILAC labeling and DNA pull-down experiments. Bio-protocol Preprint. bio-protocol.org/prep1189.
  2. Roelofs, P. A., Goh, C. Y., Chua, B. H., Jarvis, M. C., Stewart, T. A., McCann, J. L., McDougle, R. M., Carpenter, M. A., Martens, J. W., Span, P. N., Kappei, D. and Harris, R. S.(2020). Characterization of the mechanism by which the RB/E2F pathway controls expression of the cancer genomic DNA deaminase APOBEC3B. eLife. DOI: 10.7554/eLife.61287

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