Protocols shared by authors via RaP service
Journal
Culture of mouse/rat primary neurons
Authors: Richard L Huganir and Huaqiang Fang
Date: May 11, 2021，Views：1296
Original protocol used in research article：
An optimized CRISPR/Cas9 approach for precise genome editing in neurons
eLife, Mar 10, 2021; DOI: 10.7554/eLife.65202
Expression and purification of recombinant FOXO3-DBD
Authors: Judith Hagenbuchner, Tomas Obsil, Michael J Ausserlechner and Veronika Obsilova
Date: May 10, 2021，Views：813
Original protocol used in research article：
Modulating FOXO3 transcriptional activity by small, DBD-binding molecules
eLife, Dec 4, 2019; DOI: 10.7554/eLife.48876
Binning approach and normalization
Authors: Nina Kirstein, Olivier Hyrien, Benjamin Audit and Aloys Schepers
Date: May 10, 2021，Views：892
Original protocol used in research article：
Human ORC/MCM density is low in active genes and correlates with replication time but does not delimit initiation zones
eLife, Mar 8, 2021; DOI: 10.7554/eLife.62161
Protein extraction and immunobloting
Authors: Ismael Lamas-Toranzo, Julieta G Hamze, María Jiménez-Movilla and Pablo Bermejo-Álvarez
Date: May 10, 2021，Views：933
Original protocol used in research article：
TMEM95 is a sperm membrane protein essential for mammalian fertilization
eLife, Jun 2, 2020; DOI: 10.7554/eLife.53913
Immunoblotting
Authors: Benjamin Demarco, Kaiwen Chen and Petr Broz
Date: May 10, 2021，Views：875
Original protocol used in research article：
Caspase-8–dependent gasdermin D cleavage promotes antimicrobial defense but confers susceptibility to TNF-induced lethality
Science Advances, Nov 18, 2020; DOI: 10.1126/sciadv.abc3465
Gene-specific m6A qPCR
Authors: Chris Bass and Youjun Zhang
Date: May 9, 2021，Views：1259
Original protocol used in research article：
Epitranscriptomic regulation of insecticide resistance
Science Advances, May 5, 2021; DOI: 10.1126/sciadv.abe5903
Statistical analysis
Authors: David Chen, Josephine Wu and Gordana Vunjak-Novakovic
Date: May 9, 2021，Views：873
Original protocol used in research article：
Tissue engineered autologous cartilage-bone grafts for temporomandibular joint regeneration
Science Translational Medicine, Oct 13, 2020; DOI: 10.1126/scitranslmed.abb6683
3-D printing of window and clip
Authors: Qiang Huang, Rudolf Jaenisch and Xiling Shen
Date: May 7, 2021，Views：896
Original protocol used in research article：
Intravital imaging of mouse embryos
Science, Apr 9, 2020; DOI: 10.1126/science.aba0210
Sperm penetration and binding assays
Authors: Ismael Lamas-Toranzo, María Jiménez-Movilla and Pablo Bermejo-Álvarez
Date: May 7, 2021，Views：843
Original protocol used in research article：
TMEM95 is a sperm membrane protein essential for mammalian fertilization
eLife, Jun 2, 2020; DOI: 10.7554/eLife.53913
Isolation and phospholipid enrichment of muscle mitochondria and mitoplasts
Authors: Alexandre Prola, Aymeline Vandestienne, Frédéric Joubert, Laurent Tiret and Fanny Pilot-Storck
Date: May 7, 2021，Views：1139
Original protocol used in research article：
Cardiolipin content controls mitochondrial coupling and energetic efficiency in muscle
Science Advances, Dec 31, 2020; DOI: 10.1126/sciadv.abd6322
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