Improve Research Reproducibility A Bio-protocol resource
bpdetail_icon_lock
Preprint

Loading and release of siRNA from albumin NPs

MC Maria G. Castro
JL Joerg Lahann

Last updated date: Jun 17, 2021 Views: 839 Forks: 0

original An abbreviated version of this protocol was published in Nature Communications in Nov, 2020
Systemic brain tumor delivery of synthetic protein nanoparticles for glioblastoma therapy
download pdf Download PDF
ask Ask a question
cite How to cite
nfavorite Favorite

Hallo.

 

Thanks a lot for the inquiry. In our study, the AF488 dye is covalently linked with the protein nanoparticle through covalent conjugation with the human serum albumin, not the siRNA.

 

best

 

Joerg Lahann

Copyright: Content may be subjected to copyright.
How to cite：
Readers should cite both the Bio-protocol preprint and the original research article where this protocol was used:
  1. Castro, M and Lahann, J(2021). Loading and release of siRNA from albumin NPs. Bio-protocol Preprint. bio-protocol.org/prep1171.
  2. Gregory, J. V., Kadiyala, P., Doherty, R., Cadena, M., Habeel, S., Ruoslahti, E., Lowenstein, P. R., Castro, M. G. and Lahann, J.(2020). Systemic brain tumor delivery of synthetic protein nanoparticles for glioblastoma therapy. Nature Communications 11. DOI: 10.1038/s41467-020-19225-7

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

0/150

tip Tips for asking effective questions

+ Description

Write a detailed description. Include all information that will help others answer your question including experimental processes, conditions, and relevant images.

post Post a Question
0 Q&A
This protocol preprint was submitted via the "Request a Protocol" track.