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Loading and release of siRNA from albumin NPs

MC Maria G. Castro
JL Joerg Lahann

Last updated date: Jun 17, 2021 Views: 875 Forks: 0

original An abbreviated version of this protocol was published in Nature Communications in Nov, 2020
Systemic brain tumor delivery of synthetic protein nanoparticles for glioblastoma therapy
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Hallo.

 

Thanks a lot for the inquiry. In our study, the AF488 dye is covalently linked with the protein nanoparticle through covalent conjugation with the human serum albumin, not the siRNA.

 

best

 

Joerg Lahann

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How to cite：
Readers should cite both the Bio-protocol preprint and the original research article where this protocol was used:
  1. Castro, M and Lahann, J(2021). Loading and release of siRNA from albumin NPs. Bio-protocol Preprint. bio-protocol.org/prep1171.
  2. Gregory, J. V., Kadiyala, P., Doherty, R., Cadena, M., Habeel, S., Ruoslahti, E., Lowenstein, P. R., Castro, M. G. and Lahann, J.(2020). Systemic brain tumor delivery of synthetic protein nanoparticles for glioblastoma therapy. Nature Communications 11. DOI: 10.1038/s41467-020-19225-7

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