Protocols shared by authors via RaP service
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Bone sectioning, immunostaining, and confocal imaging
Authors: Lei Ding and Sean J Morrison
Date: Apr 9, 2021，Views：862
Original protocol used in research article：
Hematopoietic stem and progenitor cells regulate the regeneration of their niche by secreting Angiopoietin-1
eLife, Mar 30, 2015; DOI: 10.7554/eLife.05521
Statistical analysis
Authors: Thanaphong Phongpreecha and Thomas J. Montine
Date: Apr 8, 2021，Views：829
Original protocol used in research article：
Single-cell peripheral immunoprofiling of Alzheimer’s and Parkinson’s diseases
Science Advances, Nov 25, 2020; DOI: 10.1126/sciadv.abd5575
Contribution of inter-relatedness chimpanzees to differential expression analysis
Author: Benjamin Jung Fair
Date: Apr 8, 2021，Views：806
Original protocol used in research article：
Gene expression variability in human and chimpanzee populations share common determinants
eLife, Oct 21, 2020; DOI: 10.7554/eLife.59929
Single-nucleus RNA-seq data generation
Authors: Allen Wang, Kyle J Gaulton, Sebastian Preissl and Xin Sun
Date: Apr 7, 2021，Views：1008
Original protocol used in research article：
Single-cell multiomic profiling of human lungs reveals cell-type-specific and age-dynamic control of SARS-CoV2 host genes
eLife, Nov 9, 2020; DOI: 10.7554/eLife.62522
Microtubule co-sedimentation from worm extract
Author: Shaohe Wang
Date: Apr 7, 2021，Views：821
Original protocol used in research article：
NOCA-1 functions with γ-tubulin and in parallel to Patronin to assemble non-centrosomal microtubule arrays in C. elegans
eLife, Sep 15, 2015; DOI: 10.7554/eLife.08649
Extending 3' UTRs in the transcriptome index
Authors: Lauren M Saunders, Cole Trapnell and David M Parichy
Date: Apr 7, 2021，Views：975
Original protocol used in research article：
Thyroid hormone regulates distinct paths to maturation in pigment cell lineages
eLife, May 29, 2019; DOI: 10.7554/eLife.45181
Cell transformation assay
Authors: Karoliina Stefanius and Kim Orth
Date: Apr 7, 2021，Views：948
Original protocol used in research article：
Human pancreatic cancer cell exosomes, but not human normal cell exosomes, act as an initiator in cell transformation
eLife, May 28, 2019; DOI: 10.7554/eLife.40226
In situ hybridization
Authors: Kaiwen Kam and Carolina Thörn Perez
Date: Apr 7, 2021，Views：878
Original protocol used in research article：
Opioids modulate an emergent rhythmogenic process to depress breathing
eLife, Dec 16, 2019; DOI: 10.7554/eLife.50613
Luciferase assay
Authors: Lihui Han and dapeng ma
Date: Apr 7, 2021，Views：859
Original protocol used in research article：
Arginine methyltransferase PRMT5 negatively regulates cGAS-mediated antiviral immune response
Science Advances, Mar 24, 2021; DOI: 10.1126/sciadv.abc1834
IP and immunoblot analysis
Authors: Lihui Han and Dapeng Ma
Date: Apr 7, 2021，Views：860
Original protocol used in research article：
Arginine methyltransferase PRMT5 negatively regulates cGAS-mediated antiviral immune response
Science Advances, Mar 24, 2021; DOI: 10.1126/sciadv.abc1834
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