Protocols shared by authors via RaP service
Journal
LNP quantification and encapsulation
Author: Dan Peer
Date: Apr 19, 2021，Views：1103
Original protocol used in research article：
CRISPR-Cas9 genome editing using targeted lipid nanoparticles for cancer therapy
Science Advances, Nov 18, 2020; DOI: 10.1126/sciadv.abc9450
Flow cytometric analysis (FACS)
Authors: Andy Peng Xiang and Zhengqi Lu
Date: Apr 18, 2021，Views：824
Original protocol used in research article：
Gut microbiota from patients with arteriosclerotic CSVD induces higher IL-17A production in neutrophils via activating RORγt
Science Advances, Jan 21, 2021; DOI: 10.1126/sciadv.abe4827
Purification of cells by FACS
Authors: Hsin-I Jen and Andrew K Groves
Date: Apr 16, 2021，Views：857
Original protocol used in research article：
Transcriptomic and epigenetic regulation of hair cell regeneration in the mouse utricle and its potentiation by Atoh1
eLife, Apr 29, 2019; DOI: 10.7554/eLife.44328
RNA-seq
Authors: Hsin-I Jen and Andrew K Groves
Date: Apr 16, 2021，Views：875
Original protocol used in research article：
Transcriptomic and epigenetic regulation of hair cell regeneration in the mouse utricle and its potentiation by Atoh1
eLife, Apr 29, 2019; DOI: 10.7554/eLife.44328
Expression, purification and on-bead reconstitution of MsbA and MscS into peptidiscs
Authors: Gabriella Angiulli, Franck Duong Van Hoa and Thomas Walz
Date: Apr 16, 2021，Views：1011
Original protocol used in research article：
New approach for membrane protein reconstitution into peptidiscs and basis for their adaptability to different proteins
eLife, Mar 3, 2020; DOI: 10.7554/eLife.53530
Open-field test
Authors: Byung Tae Choi and Da Hee Jung
Date: Apr 16, 2021，Views：1740
Original protocol used in research article：
Therapeutic effects of anodal transcranial direct current stimulation in a rat model of ADHD
eLife, Sep 21, 2020; DOI: 10.7554/eLife.56359
Chronic mild stress
Authors: Huafu Chen and Zili You
Date: Apr 15, 2021，Views：954
Original protocol used in research article：
IL4-driven microglia modulate stress resilience through BDNF-dependent neurogenesis
Science Advances, Mar 17, 2021; DOI: 10.1126/sciadv.abb9888
Computational design of antibody nanocages (AbCs)
Authors: Robby Divine and David Baker
Date: Apr 15, 2021，Views：904
Original protocol used in research article：
Designed proteins assemble antibodies into modular nanocages
Science, Apr 2, 2021; DOI: 10.1126/science.abd9994
Synthesis of germanium nanospheres as trappable optical probes (GeNTOPs)
Authors: Swathi Sudhakar, Viktoria Wedler and Erik Schäffer
Date: Apr 15, 2021，Views：942
Original protocol used in research article：
Germanium nanospheres for ultraresolution picotensiometry of kinesin motors
Science, Feb 11, 2021; DOI: 10.1126/science.abd9944
B cell and T cell phenotyping
Author: Guy Gorochov
Date: Apr 14, 2021，Views：1022
Original protocol used in research article：
IgA dominates the early neutralizing antibody response to SARS-CoV-2
Science Translational Medicine, Jan 20, 2021; DOI: 10.1126/scitranslmed.abd2223
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