Protocols shared by authors via RaP service
Journal
mtDNA extraction from Fr8 EVs and qPCR
Author: Efrat Levy
Date: Apr 28, 2021，Views：900
Original protocol used in research article：
Mitovesicles are a novel population of extracellular vesicles of mitochondrial origin altered in Down syndrome
Science Advances, Feb 11, 2021; DOI: 10.1126/sciadv.abe5085
In-situ proximity ligation
Authors: Konrad Basler, Andreas E Moor, Claudio Cantù and Amaia Jauregi-Miguel
Date: Apr 28, 2021，Views：822
Original protocol used in research article：
TBX3 acts as tissue-specific component of the Wnt/β-catenin transcriptional complex
eLife, Aug 18, 2020; DOI: 10.7554/eLife.58123
Microfabrication of microfluidic molds for layer-specific microchannel networks
Author: Tyler R Ray
Date: Apr 27, 2021，Views：853
Original protocol used in research article：
Soft, skin-interfaced sweat stickers for cystic fibrosis diagnosis and management
Science Translational Medicine, Mar 31, 2021; DOI: 10.1126/scitranslmed.abd8109
Neural induction
Authors: G. Zhao and J. Fu
Date: Apr 26, 2021，Views：895
Original protocol used in research article：
Dorsal-ventral patterned neural cyst from human pluripotent stem cells in a neurogenic niche
Science Advances, Dec 10, 2019; DOI: 10.1126/sciadv.aax5933
Fabrication of gel beds
Authors: G. Zhao and J. Fu
Date: Apr 26, 2021，Views：783
Original protocol used in research article：
Dorsal-ventral patterned neural cyst from human pluripotent stem cells in a neurogenic niche
Science Advances, Dec 10, 2019; DOI: 10.1126/sciadv.aax5933
Confocal microscopy and analysis
Authors: Maria D Purice, Sean D Speese and Mary A Logan
Date: Apr 26, 2021，Views：1353
Original protocol used in research article：
A novel Drosophila injury model reveals severed axons are cleared through a Draper/MMP-1 signaling cascade
eLife, Aug 21, 2017; DOI: 10.7554/eLife.23611
CNV detection
Authors: Jean-Laurent Casanova and Qian Zhang
Date: Apr 26, 2021，Views：898
Original protocol used in research article：
Inborn errors of type I IFN immunity in patients with life-threatening COVID-19
Science, Sep 24, 2020; DOI: 10.1126/science.abd4570
Influenza virus protein
Authors: Jean-Laurent Casanova and Qian Zhang
Date: Apr 26, 2021，Views：1014
Original protocol used in research article：
Inborn errors of type I IFN immunity in patients with life-threatening COVID-19
Science, Sep 24, 2020; DOI: 10.1126/science.abd4570
Patients
Authors: Qian Zhang and Jean-Laurent Casanova
Date: Apr 26, 2021，Views：1005
Original protocol used in research article：
Inborn errors of type I IFN immunity in patients with life-threatening COVID-19
Science, Sep 24, 2020; DOI: 10.1126/science.abd4570
Mouse brain stereotaxic injections
Authors: Huda Y. Zoghbi, Yingyao Shao and Yehezkel Sztainberg
Date: Apr 26, 2021，Views：2660
Original protocol used in research article：
Antisense oligonucleotide therapy in a humanized mouse model of MECP2 duplication syndrome
Science Translational Medicine, Mar 2, 2021; DOI: 10.1126/scitranslmed.aaz7785
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