Protocols shared by authors via RaP service
Journal
FUCCI live-cell imaging
Author: Andrew Burgess
Date: May 6, 2021，Views：948
Original protocol used in research article：
Analysis of pulsed cisplatin signalling dynamics identifies effectors of resistance in lung adenocarcinoma
eLife, Jun 9, 2020; DOI: 10.7554/eLife.53367
PhosFate analysis
Authors: Jonathan M Budzik and Jeffery S Cox
Date: May 6, 2021，Views：903
Original protocol used in research article：
Dynamic post-translational modification profiling of Mycobacterium tuberculosis-infected primary macrophages
eLife, Jan 17, 2020; DOI: 10.7554/eLife.51461
Web app
Authors: Jonathan M Budzik and Jeffery S Cox
Date: May 6, 2021，Views：905
Original protocol used in research article：
Dynamic post-translational modification profiling of Mycobacterium tuberculosis-infected primary macrophages
eLife, Jan 17, 2020; DOI: 10.7554/eLife.51461
RNAseq
Authors: Jonathan M Budzik and Jeffery S Cox
Date: May 6, 2021，Views：830
Original protocol used in research article：
Dynamic post-translational modification profiling of Mycobacterium tuberculosis-infected primary macrophages
eLife, Jan 17, 2020; DOI: 10.7554/eLife.51461
Immunofluorescence microscopy for LC3
Authors: Jonathan M Budzik and Jeffery S Cox
Date: May 6, 2021，Views：955
Original protocol used in research article：
Dynamic post-translational modification profiling of Mycobacterium tuberculosis-infected primary macrophages
eLife, Jan 17, 2020; DOI: 10.7554/eLife.51461
ATAC-seq
Authors: Heinrich Jasper and Helen Marie Tauc
Date: May 6, 2021，Views：941
Original protocol used in research article：
Age-related changes in polycomb gene regulation disrupt lineage fidelity in intestinal stem cells
eLife, Mar 16, 2021; DOI: 10.7554/eLife.62250
Post-translational histone modifications panel (active motif)
Authors: Heinrich Jasper and Helen Marie Tauc
Date: May 6, 2021，Views：856
Original protocol used in research article：
Age-related changes in polycomb gene regulation disrupt lineage fidelity in intestinal stem cells
eLife, Mar 16, 2021; DOI: 10.7554/eLife.62250
In vitro permeability assay
Authors: Shima Ghoroghi, Jacky G Goetz and Vincent Hyenne
Date: May 6, 2021，Views：1343
Original protocol used in research article：
Ral GTPases promote breast cancer metastasis by controlling biogenesis and organ targeting of exosomes
eLife, Jan 6, 2021; DOI: 10.7554/eLife.61539
IP and MS
Author: Xiaoming Zhang
Date: May 6, 2021，Views：983
Original protocol used in research article：
DEAD-box helicases modulate dicing body formation in Arabidopsis
Science Advances, Apr 28, 2021; DOI: 10.1126/sciadv.abc6266
Single-cell tissue preparation
Authors: Kristen L Wells, Mark S Anderson, Lars M Steinmetz and Corey N Miller
Date: May 4, 2021，Views：917
Original protocol used in research article：
Combined transient ablation and single-cell RNA-sequencing reveals the development of medullary thymic epithelial cells
eLife, Nov 23, 2020; DOI: 10.7554/eLife.60188
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