Protocols shared by authors via RaP service
Journal
Microscopy and live-cell imaging
Author: Perunthottathu K Umasankar
Date: May 12, 2021，Views：961
Original protocol used in research article：
A clathrin coat assembly role for the muniscin protein central linker revealed by TALEN-mediated gene editing
eLife, Oct 10, 2014; DOI: 10.7554/eLife.04137
Cell culture, transfections and immunofluorescence
Author: Perunthottathu K Umasankar
Date: May 12, 2021，Views：1475
Original protocol used in research article：
A clathrin coat assembly role for the muniscin protein central linker revealed by TALEN-mediated gene editing
eLife, Oct 10, 2014; DOI: 10.7554/eLife.04137
Database construction
Authors: Daniel A Russell and Graham F Hatfull
Date: May 12, 2021，Views：959
Original protocol used in research article：
Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity
eLife, Apr 28, 2015; DOI: 10.7554/eLife.06416
Cluster diversity and isolation indices
Authors: Daniel A Russell and Graham F Hatfull
Date: May 12, 2021，Views：934
Original protocol used in research article：
Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity
eLife, Apr 28, 2015; DOI: 10.7554/eLife.06416
Histology: preparation of samples for FFPE or frozen sectioning
Author: Jessica E Ackerman
Date: May 12, 2021，Views：935
Original protocol used in research article：
Cell non-autonomous functions of S100a4 drive fibrotic tendon healing
eLife, May 24, 2019; DOI: 10.7554/eLife.45342
co-immunoprecipitation and immunoblotting
Authors: Taifeng Zhou, Peiqiang Su and Yingzi Yang
Date: May 12, 2021，Views：2616
Original protocol used in research article：
Piezo1/2 mediate mechanotransduction essential for bone formation through concerted activation of NFAT-YAP1-ß-catenin
eLife, Mar 18, 2020; DOI: 10.7554/eLife.52779
T cell isolation
Authors: Scott B Thompson and Jordan Jacobelli
Date: May 11, 2021，Views：1197
Original protocol used in research article：
Formin-like 1 mediates effector T cell trafficking to inflammatory sites to enable T cell-mediated autoimmunity
eLife, Jun 8, 2020; DOI: 10.7554/eLife.58046
Barley spikelet morphometry analysis
Author: Ravi Koppolu
Date: May 11, 2021，Views：896
Original protocol used in research article：
Transcriptional landscapes of floral meristems in barley
Science Advances, Apr 28, 2021; DOI: 10.1126/sciadv.abf0832
Culture of mouse/rat primary neurons
Authors: Richard L Huganir and Huaqiang Fang
Date: May 11, 2021，Views：1427
Original protocol used in research article：
An optimized CRISPR/Cas9 approach for precise genome editing in neurons
eLife, Mar 10, 2021; DOI: 10.7554/eLife.65202
Expression and purification of recombinant FOXO3-DBD
Authors: Judith Hagenbuchner, Tomas Obsil, Michael J Ausserlechner and Veronika Obsilova
Date: May 10, 2021，Views：841
Original protocol used in research article：
Modulating FOXO3 transcriptional activity by small, DBD-binding molecules
eLife, Dec 4, 2019; DOI: 10.7554/eLife.48876
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