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Preprint

Optic fiber implantation

MA Mehran Ahmadlou
JH J. Alexander Heimel

Last updated date: Jun 9, 2021 Views: 842 Forks: 0

original An abbreviated version of this protocol was published in Science in May, 2021
A cell type–specific cortico-subcortical brain circuit for investigatory and novelty-seeking behavior
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  1. 10-15 minutes wait after virus injection (using nanoject II or III)
     
  2. using a holder connected to stereotactic arm slowly lower down (e.g. 0.5 mm / min) the fiber optic (e.g. ZF1.25 200um fiber) and stop 100-200 um above the region of interest and cement it to the skull (e.g. using optibond and tetric flow light-cure cement). Cement should cover 40-60% of the ferrule (fiber cannula).
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How to cite：
Readers should cite both the Bio-protocol preprint and the original research article where this protocol was used:
  1. Ahmadlou, M and Heimel, J(2021). Optic fiber implantation. Bio-protocol Preprint. bio-protocol.org/prep1146.
  2. Ahmadlou, M., Houba, J. H. W., Vierbergen, J. F. M. V., Giannouli, M., Gimenez, G., Weeghel, C. V., Darbanfouladi, M., Shirazi, M. Y., Dziubek, J., Kacem, M., Winter, F. D. and Heimel, J. A.(2021). A cell type–specific cortico-subcortical brain circuit for investigatory and novelty-seeking behavior. Science 372(6543). DOI: 10.1126/science.abe9681

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