Quantification of brain infarct volume

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The following is the specific protocol to calculate the volume of cerebral infarction:

The brain tissue is taken out and placed in a brain mold, and then cut into 5 slices with a thickness of 2mm/slice continuously through the coronal plane, and then stained with TTC, then fixed with 4% paraformaldehyde for 24 hours, and absorb the surface water with absorbent paper Stains, and finally put it into the scanner to scan and access the picture. Analyze the pictures with Image J software to calculate the area of cerebral infarction and normal tissue, and use the following formula to calculate the percentage of cerebral infarction volume:

Hemisphere volume=[(front area+reverse area)/2]×slice thickness.

Percentage of total brain infarct volume=(∑(contralateral normal tissue volume-ipsilateral normal tissue volume))/(∑contralateral normal tissue volume×2)×100%

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