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Assays of cellular stress.
Last updated date: Jun 22, 2021 Views: 816 Forks: 0
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1. Fresh conidia of the WT and mutant strains were harvested from cultures grown on mCDB plates at 28° C for 12 days and afterwards filtered using sterile Mira cloth.
2. 1 ul of conidia suspension containing 5000 conidia of the WT or mutant strains were inoculated on mCDB plates previously supplemented with 0.8 M NACl and 1 M KCl for osmotic stress assay.
3. For oxidative and cell wall perturbing stress, same procedure in step (2) was repeated.
4. All cultures were incubated at 28° C for 4 days.
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How to cite：
Readers should cite both the Bio-protocol preprint and the original research article where this protocol was used:
- Igbalajobi, O, Yu, Z and Fischer, R(2021). Assays of cellular stress.. Bio-protocol Preprint. bio-protocol.org/prep1183.
- Igbalajobi, O., Yu, Z. and Fischer, R.(2019). Red- and Blue-Light Sensing in the Plant Pathogen Alternaria alternata Depends on Phytochrome and the White-Collar Protein LreA. mBio 10(2). DOI: 10.1128/mBio.00371-19
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