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Preprint

Real-Time PCR

MX Ming Xu
JK James L Kirkland

Last updated date: Jun 3, 2021 Views: 972 Forks: 0

original An abbreviated version of this protocol was published in eLife in Dec, 2015
Targeting senescent cells enhances adipogenesis and metabolic function in old age
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All primers were purchased from IDT. The catalog numbers are listed below.

 

Sequence NameSequence Notes
Hs.PT.58.20106818FABP4, HOMO_SAPIENS
Hs.PT.58.20757758INHBA, HOMO_SAPIENS
Hs.PT.58.25464465PPARG, HOMO_SAPIENS
Hs.PT.58.38657761PLIN1, HOMO_SAPIENS
Hs.PT.58.4022335.gCEBPA, HOMO_SAPIENS
Hs.PT.58v.39858774TBP, HOMO_SAPIENS
  
Mm.PT.39a.22214839TBP, MUS_MUSCULUS
Mm.PT.58.10000337Plin1, MUS_MUSCULUS
Mm.PT.58.10005566IL6, MUS_MUSCULUS
Mm.PT.58.17125846CDKN1A, MUS_MUSCULUS
Mm.PT.58.31161924PPARg, MUS_MUSCULUS
Mm.PT.58.42076891CXCL1, MUS_MUSCULUS
Mm.PT.58.42151692CCL2, MUS_MUSCULUS
Mm.PT.58.43866459FABP4, MUS_MUSCULUS
Mm.PT.58.6413525SERPINE1, MUS_MUSCULUS
Mm.PT.58.8388138CDKN2A, MUS_MUSCULUS
Mm.PT.58.9719546AdipoQ, MUS_MUSCULUS

 

 

 

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How to cite：
Readers should cite both the Bio-protocol preprint and the original research article where this protocol was used:
  1. Xu, M and Kirkland, J(2021). Real-Time PCR. Bio-protocol Preprint. bio-protocol.org/prep1135.
  2. Xu, M., Palmer, A. K., Ding, H., Weivoda, M. M., Pirtskhalava, T., White, T. A., Sepe, A., Johnson, K. O., Stout, M. B., Giorgadze, N., Jensen, M. D., LeBrasseur, N. K., Tchkonia, T. and Kirkland, J. L.(2015). Targeting senescent cells enhances adipogenesis and metabolic function in old age. eLife. DOI: 10.7554/eLife.12997

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