All primers were purchased from IDT. The catalog numbers are listed below.
Sequence Name Sequence Notes Hs.PT.58.20106818 FABP4, HOMO_SAPIENS Hs.PT.58.20757758 INHBA, HOMO_SAPIENS Hs.PT.58.25464465 PPARG, HOMO_SAPIENS Hs.PT.58.38657761 PLIN1, HOMO_SAPIENS Hs.PT.58.4022335.g CEBPA, HOMO_SAPIENS Hs.PT.58v.39858774 TBP, HOMO_SAPIENS Mm.PT.39a.22214839 TBP, MUS_MUSCULUS Mm.PT.58.10000337 Plin1, MUS_MUSCULUS Mm.PT.58.10005566 IL6, MUS_MUSCULUS Mm.PT.58.17125846 CDKN1A, MUS_MUSCULUS Mm.PT.58.31161924 PPARg, MUS_MUSCULUS Mm.PT.58.42076891 CXCL1, MUS_MUSCULUS Mm.PT.58.42151692 CCL2, MUS_MUSCULUS Mm.PT.58.43866459 FABP4, MUS_MUSCULUS Mm.PT.58.6413525 SERPINE1, MUS_MUSCULUS Mm.PT.58.8388138 CDKN2A, MUS_MUSCULUS Mm.PT.58.9719546 AdipoQ, MUS_MUSCULUS
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How to cite: Readers should cite both the Bio-protocol preprint and the original research article where this protocol was used:
Xu, M and Kirkland, J(2021). Real-Time PCR. Bio-protocol Preprint. bio-protocol.org/prep1135 . Xu, M., Palmer, A. K., Ding, H., Weivoda, M. M., Pirtskhalava, T., White, T. A., Sepe, A., Johnson, K. O., Stout, M. B., Giorgadze, N., Jensen, M. D., LeBrasseur, N. K., Tchkonia, T. and Kirkland, J. L.(2015). Targeting senescent cells enhances adipogenesis and metabolic function in old age. eLife. DOI: 10.7554/eLife.12997
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