Protocols shared by authors via RaP service
Journal
Intestinal organoid cultures
Authors: Zuri Sullivan and Ruslan Medzhitov
Date: May 18, 2021，Views：871
Original protocol used in research article：
γδ T cells regulate the intestinal response to nutrient sensing
Science, Mar 19, 2021; DOI: 10.1126/science.aba8310
Data filtering and genome assembly
Authors: Reuben W Nowell and Timothy G Barraclough
Date: May 18, 2021，Views：828
Original protocol used in research article：
Evolutionary dynamics of transposable elements in bdelloid rotifers
eLife, Feb 5, 2021; DOI: 10.7554/eLife.63194
Data
Authors: Li Wang and Ni Huang
Date: May 17, 2021，Views：936
Original protocol used in research article：
Spatial and temporal variations in global soil respiration and their relationships with climate and land cover
Science Advances, Oct 6, 2020; DOI: 10.1126/sciadv.abb8508
Parameter optimizations of the three machine learning algorithm models
Authors: Ni Huang and Li Wang
Date: May 17, 2021，Views：850
Original protocol used in research article：
Spatial and temporal variations in global soil respiration and their relationships with climate and land cover
Science Advances, Oct 6, 2020; DOI: 10.1126/sciadv.abb8508
Whole Mount in situ Hybridization (WISH) on mouse embryos
Authors: Matthew J Anderson and Mark Lewandoski
Date: May 17, 2021，Views：839
Original protocol used in research article：
Fgf4 maintains Hes7 levels critical for normal somite segmentation clock function
eLife, Nov 19, 2020; DOI: 10.7554/eLife.55608
Characterization of CAR-transduced T cell cytotoxic potential
Authors: Vanaja Konduri and William K. Decker
Date: May 17, 2021，Views：889
Original protocol used in research article：
A subset of cytotoxic effector memory T cells enhances CAR T cell efficacy in a model of pancreatic ductal adenocarcinoma
Science Translational Medicine, May 5, 2021; DOI: 10.1126/scitranslmed.abc3196
Immunofluorescence, Sirius red staining, and intensity quantitations
Authors: Denise Gay, Paul-Henri Romeo and Denise Gay
Date: May 16, 2021，Views：900
Original protocol used in research article：
Phagocytosis of Wnt inhibitor SFRP4 by late wound macrophages drives chronic Wnt activity for fibrotic skin healing
Science Advances, Mar 19, 2020; DOI: 10.1126/sciadv.aay3704
T cell migration assays
Author: Dong Wang
Date: May 16, 2021，Views：960
Original protocol used in research article：
Tumor immunological phenotype signature-based high-throughput screening for the discovery of combination immunotherapy compounds
Science Advances, Jan 21, 2021; DOI: 10.1126/sciadv.abd7851
Identification of gene loss
Authors: Rasmus Heller, Wen Wang, Jinghui Huang and Qiang Qiu
Date: May 15, 2021，Views：903
Original protocol used in research article：
A towering genome: Experimentally validated adaptations to high blood pressure and extreme stature in the giraffe
Science Advances, Mar 17, 2021; DOI: 10.1126/sciadv.abe9459
Gene family expansion and contraction
Authors: Rasmus Heller, Wen Wang, Jinghui Huang and Qiang Qiu
Date: May 15, 2021，Views：931
Original protocol used in research article：
A towering genome: Experimentally validated adaptations to high blood pressure and extreme stature in the giraffe
Science Advances, Mar 17, 2021; DOI: 10.1126/sciadv.abe9459
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