Protocols shared by authors via RaP service
Journal
Cell-free BoDV preparation.
Authors: Keizo Tomonaga and Akiko Makino
Date: Jun 25, 2021，Views：760
Original protocol used in research article：
ADAR2 Is Involved in Self and Nonself Recognition of Borna Disease Virus Genomic RNA in the Nucleus
Journal of Virology, Feb 28, 2020; DOI: 10.1128/JVI.01513-19
AAV vector production
Authors: Sean K Wang and Constance L. Cepko
Date: Jun 25, 2021，Views：879
Original protocol used in research article：
Microglia modulation by TGF-β1 protects cones in mouse models of retinal degeneration
The Journal of Clinical Investigation, Jul 13, 2020; DOI: 10.1172/JCI136160
Primary neuron-astrocyte co-culture
Authors: Michelle L Olsen and Raymundo D Hernandez
Date: Jun 25, 2021，Views：777
Original protocol used in research article：
Astrocyte morphogenesis is dependent on BDNF signaling via astrocytic TrkB.T1
eLife, Aug 21, 2019; DOI: 10.7554/eLife.44667
Generation of In vitro Derived Mucus
Authors: Lisa H. Cazares and Sonja Hess
Date: Jun 25, 2021，Views：741
Original protocol used in research article：
Development of a Parallel Reaction Monitoring Mass Spectrometry Assay for the Detection of SARS-CoV-2 Spike Glycoprotein and Nucleoprotein
Anal Chem, Sep 23, 2020; DOI: 10.1021/acs.analchem.0c02288
Linearity of the assay
Author: Nicolas Chomont
Date: Jun 25, 2021，Views：749
Original protocol used in research article：
Single-cell characterization and quantification of translation-competent viral reservoirs in treated and untreated HIV infection
PLoS Pathogens, Feb 27, 2019; DOI: 10.1371/journal.ppat.1007619
Isolation, culture and flow cytometry of bone marrow B cell progenitors.
Authors: Malay Mandal and Marcus R Clark
Date: Jun 24, 2021，Views：720
Original protocol used in research article：
CXCR4 signaling directs Igk recombination and the molecular mechanisms of late B lymphopoiesis
Nat Immunol, Sep 2, 2019; DOI: 10.1038/s41590-019-0468-0
FRET-based nucleosomes sliding
Authors: Robert L. Yauch and Tharu Fernando
Date: Jun 24, 2021，Views：731
Original protocol used in research article：
Functional characterization of SMARCA4 variants identified by targeted exome-sequencing of 131,668 cancer patients
Nature Communications, Nov 3, 2020; DOI: 10.1038/s41467-020-19402-8
TRITC-transferrin internalization assay
Author: Feng Liu
Date: Jun 24, 2021，Views：799
Original protocol used in research article：
Rab5c-mediated endocytic trafficking regulates hematopoietic stem and progenitor cell development via Notch and AKT signaling
PLoS Biology, Apr 10, 2020; DOI: 10.1371/journal.pbio.3000696
In vitro MV uptake assay
Authors: Ying Ying Tan, Kieran P. O’Dea and Masao Takata
Date: Jun 24, 2021，Views：958
Original protocol used in research article：
Monocytes mediate homing of circulating microvesicles to the pulmonary vasculature during low-grade systemic inflammation
Journal of Extracellular Vesicles, Jan 5, 2020; DOI: 10.1080/20013078.2019.1706708
Measuring Endogenous Phytohormones
Authors: Yunping Chen, Zhiwu Dan and Shaoqing Li
Date: Jun 24, 2021，Views：993
Original protocol used in research article：
Rice GROWTH-REGULATING FACTOR7 Modulates Plant Architecture through Regulating GA and Indole-3-Acetic Acid Metabolism1,[OPEN]
Plant Physiology, Jun 24, 2020; DOI: 10.1104/pp.20.00302
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