Protocols shared by authors via RaP service
Journal
ATAC-seq and ChIP-seq data analysis on mouse strains data
Authors: Zeyang Shen, Marten A Hoeksema and Christopher K. Glass
Date: Jul 8, 2021，Views：1183
Original protocol used in research article：
Mechanisms underlying divergent responses of genetically distinct macrophages to IL-4
Science Advances, Jun 16, 2021; DOI: 10.1126/sciadv.abf9808
Protocol-BODIPY FL C5-Sphingomyelin or BODIPY FL C5-Ceramide staining in mESCs
Authors: Wei Fan and Xiaoling Li
Date: Jul 7, 2021，Views：1268
Original protocol used in research article：
SIRT1 regulates sphingolipid metabolism and neural differentiation of mouse embryonic stem cells through c-Myc-SMPDL3B
eLife, May 27, 2021; DOI: 10.7554/eLife.67452
Spheroid-formation assay
Authors: Karen Legler and Leticia Oliveira-Ferrer
Date: Jul 7, 2021，Views：871
Original protocol used in research article：
Prognostic relevance of the Golgi mannosidase MAN1A1 in ovarian cancer: impact of N-glycosylation on tumour cell aggregation
British Journal of Cancer, Oct 29, 2019; DOI: 10.1038/s41416-019-0607-2
Cell-free degradation assays
Authors: Qingyan Shu and Liangsheng Wang
Date: Jul 7, 2021，Views：1607
Original protocol used in research article：
Chalcone synthase is ubiquitinated and degraded via interactions with a RING-H2 protein in petals of Paeonia ‘He Xie’
Journal of Experimental Botany, May 18, 2019; DOI: 10.1093/jxb/erz245
Labile Zinc Measurement by Flow Cytometry
Authors: Aleksha Panwar and Guruprasad R. Medigeshi
Date: Jul 6, 2021，Views：859
Original protocol used in research article：
Zinc Chelation Specifically Inhibits Early Stages of Dengue Virus Replication by Activation of NF-κB and Induction of Antiviral Response in Epithelial Cells
Frontiers in Immunology, Oct 1, 2019; DOI: 10.3389/fimmu.2019.02347
Immunoassay analysis of poly(GP)
Authors: Nadja S. Andrade and Zane Zeier
Date: Jul 6, 2021，Views：804
Original protocol used in research article：
Dipeptide repeat proteins inhibit homology-directed DNA double strand break repair in C9ORF72 ALS/FTD
Molecular Neurodegeneration, Feb 24, 2020; DOI: 10.1186/s13024-020-00365-9
Mitochondrial pyruvate transport assay
Authors: Robinson Arce-Molina, L Felipe Barros and Alejandro San Martín
Date: Jul 6, 2021，Views：979
Original protocol used in research article：
A highly responsive pyruvate sensor reveals pathway-regulatory role of the mitochondrial pyruvate carrier MPC
eLife, Mar 6, 2020; DOI: 10.7554/eLife.53917
Sequential CNS population isolations
Authors: Michelle L Olsen and Raymundo D Hernandez
Date: Jul 6, 2021，Views：762
Original protocol used in research article：
Astrocyte morphogenesis is dependent on BDNF signaling via astrocytic TrkB.T1
eLife, Aug 21, 2019; DOI: 10.7554/eLife.44667
2.7. Efferocytosis assay
Author: Vladimir Leksa
Date: Jul 6, 2021，Views：916
Original protocol used in research article：
The mannose 6‐phosphate/insulin‐like growth factor 2 receptor mediates plasminogen‐induced efferocytosis
Journal of Leukocyte Biology, Jan 18, 2019; DOI: 10.1002/JLB.1AB0417-160RR
Complex preparation and crystallization
Authors: Shuguang Tan, Hui Feng and Jinghua Yan
Date: Jul 5, 2021，Views：807
Original protocol used in research article：
Glycosylation-independent binding of monoclonal antibody toripalimab to FG loop of PD-1 for tumor immune checkpoint therapy
mAbs, Apr 19, 2019; DOI: 10.1080/19420862.2019.1596513
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