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Adhesion scoring, whole-mount imaging, and tissue clearing

JZ J. Zindel
PK P. Kubes

Last updated date: Jan 11, 2022 Views: 703 Forks: 0

original An abbreviated version of this protocol was published in Science in Mar, 2021
Primordial GATA6 macrophages function as extravascular platelets in sterile injury
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Thank you for the question. For this paper we used a well established adhesion score that scores adhesions based on their tensile strength and vascularization (table S1). We do not claim novelty for this score which has been previously described by Zuhlke et. al. (Reference 50 in the Paper) and has since then been extensively used (https://pubmed.ncbi.nlm.nih.gov/?linkname=pubmed_pubmed_citedin&from_uid=1983476).

 

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Readers should cite both the Bio-protocol preprint and the original research article where this protocol was used:
  1. Zindel, J and Kubes, P(2022). Adhesion scoring, whole-mount imaging, and tissue clearing. Bio-protocol Preprint. bio-protocol.org/prep1494.
  2. Zindel, J., Peiseler, M., Hossain, M., Deppermann, C., Lee, W. Y., Haenni, B., Zuber, B., Deniset, J. F., Surewaard, B. G. J., Candinas, D. and Kubes, P.(2021). Primordial GATA6 macrophages function as extravascular platelets in sterile injury . Science 371(6533). DOI: 10.1126/science.abe0595

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