Bio-101 - Improve Research Reproducibility

Larval Locomotion Assay

Authors: Lilian A. Patron and Konrad E. Zinsmaier
Date: Jun 23, 2021，Views：766

Original protocol used in research article：

Cul4 ubiquitin ligase cofactor DCAF12 promotes neurotransmitter release and homeostatic plasticity
The Journal of Cell Biology, Mar 4, 2019; DOI: 10.1083/jcb.201805099

H&E Staining, Masson Trichrome Staining, and Immunohistochemistry of Kidney Tissues

Authors: Ying-Jan Wang and Rong-Jane Chen
Date: Jun 23, 2021，Views：941

Original protocol used in research article：

Induction of Autophagy by Pterostilbene Contributes to the Prevention of Renal Fibrosis via Attenuating NLRP3 Inflammasome Activation and Epithelial-Mesenchymal Transition
Frontiers in Cell and Developmental Biology, Jan 1, 2020; DOI: 10.3389/fcell.2020.00436

Colon loop ligation assay.

Authors: Liang Tao, Min Dong and jie Zhang
Date: Jun 23, 2021，Views：786

Original protocol used in research article：

Sulfated glycosaminoglycans and low-density lipoprotein receptor contribute to Clostridium difficile toxin A entry into cells
Nat Microbiol, Jun 3, 2019; DOI: 10.1038/s41564-019-0464-z

Sorting of antigen-specific single B cells by flow cytometry

Author: Wei Jiang
Date: Jun 23, 2021，Views：801

Original protocol used in research article：

Systemic translocation of Staphylococcus drives autoantibody production in HIV disease
Microbiome, Feb 14, 2019; DOI: 10.1186/s40168-019-0646-1

Serum-free primary astrocyte culture

Author: Raymundo D Hernandez
Date: Jun 23, 2021，Views：713

Original protocol used in research article：

Astrocyte morphogenesis is dependent on BDNF signaling via astrocytic TrkB.T1
eLife, Aug 21, 2019; DOI: 10.7554/eLife.44667

BV2 Microglial Culture and Microglial Exosome Isolation

Author: Yusheng Wang
Date: Jun 23, 2021，Views：910

Original protocol used in research article：

Exosomes from Microglia Attenuate Photoreceptor Injury and Neovascularization in an Animal Model of Retinopathy of Prematurity
Molecular Therapy. Nucleic Acids, May 17, 2019; DOI: 10.1016/j.omtn.2019.04.029

SILAC labeling and DNA pull-down experiments

Authors: Reuben S Harris and Dennis Kappei
Date: Jun 23, 2021，Views：886

Original protocol used in research article：

Characterization of the mechanism by which the RB/E2F pathway controls expression of the cancer genomic DNA deaminase APOBEC3B
eLife, Sep 28, 2020; DOI: 10.7554/eLife.61287

In vitro BBB penetration of NPs

Authors: Nitin Joshi and Sezin Aday
Date: Jun 23, 2021，Views：1017

Original protocol used in research article：

BBB pathophysiology–independent delivery of siRNA in traumatic brain injury
Science Advances, Dec 31, 2020; DOI: 10.1126/sciadv.abd6889

2.7. In Vivo Wounding Assay

Author: Vladimir Krylov
Date: Jun 23, 2021，Views：780

Original protocol used in research article：

Epithelial-Mesenchymal Transition Promotes the Differentiation Potential of Xenopus tropicalis Immature Sertoli Cells
Stem Cells International, May 5, 2019; DOI: 10.1155/2019/8387478

ATG5 siRNA Transfection

Author: Anna-Mart Engelbrecht
Date: Jun 23, 2021，Views：817

Original protocol used in research article：

Amino Acid Starvation Sensitizes Resistant Breast Cancer to Doxorubicin-Induced Cell Death
Frontiers in Cell and Developmental Biology, Oct 15, 2020; DOI: 10.3389/fcell.2020.565915

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