Protocols shared by authors via RaP service
Journal
Complex preparation and crystallization
Authors: Shuguang Tan, Hui Feng and Jinghua Yan
Date: Jul 5, 2021，Views：807
Original protocol used in research article：
Glycosylation-independent binding of monoclonal antibody toripalimab to FG loop of PD-1 for tumor immune checkpoint therapy
mAbs, Apr 19, 2019; DOI: 10.1080/19420862.2019.1596513
Isolation of stem cells
Author: Anna Halling Folkmar Andersen
Date: Jul 5, 2021，Views：820
Original protocol used in research article：
Comparable human reconstitution following Cesium-137 versus X-ray irradiation preconditioning in immunodeficient NOG mice
PLoS ONE, Oct 29, 2020; DOI: 10.1371/journal.pone.0241375
2.7. Purification of VEEV-RABV-G virions
Authors: Ya-Nan Zhang and Bo Zhang
Date: Jul 4, 2021，Views：821
Original protocol used in research article：
A novel rabies vaccine based on infectious propagating particles derived from hybrid VEEV-Rabies replicon
EBioMedicine, Jun 5, 2020; DOI: 10.1016/j.ebiom.2020.102819
Typhoid toxin disassembly assay
Author: SHU-JUNG CHANG
Date: Jul 4, 2021，Views：818
Original protocol used in research article：
Unique features in the intracellular transport of typhoid toxin revealed by a genome-wide screen
PLoS Pathogens, Apr 5, 2019; DOI: 10.1371/journal.ppat.1007704
Typhoid toxin retro-translocation assay
Author: SHU-JUNG CHANG
Date: Jul 4, 2021，Views：778
Original protocol used in research article：
Unique features in the intracellular transport of typhoid toxin revealed by a genome-wide screen
PLoS Pathogens, Apr 5, 2019; DOI: 10.1371/journal.ppat.1007704
Viral Polymerase Activity Assay
Authors: Lianzhong Zhao and Meilin Jin
Date: Jul 4, 2021，Views：817
Original protocol used in research article：
Avian Chaperonin Containing TCP1 Subunit 5 Supports Influenza A Virus Replication by Interacting With Viral Nucleoprotein, PB1, and PB2 Proteins
Frontiers in Microbiology, Oct 15, 2020; DOI: 10.3389/fmicb.2020.538355
Detection of phospho-epitopes on human NK cells for cell signaling analysis
Authors: Niklas K. Björkström and Martin Cornillet
Date: Jul 4, 2021，Views：848
Original protocol used in research article：
NK cells are activated and primed for skin-homing during acute dengue virus infection in humans
Nature Communications, Aug 29, 2019; DOI: 10.1038/s41467-019-11878-3
Spontaneous and lineage-directed differentiation of hESCs and hiPSCs
Authors: Jiwon Jang and Kenneth S. Kosik
Date: Jul 3, 2021，Views：821
Original protocol used in research article：
Control over single-cell distribution of G1 lengths by WNT governs pluripotency
PLoS Biology, Sep 26, 2019; DOI: 10.1371/journal.pbio.3000453
2.14. Isolation of Primary Liver Sinusoidal Endothelial Cells
Author: Hannah K. Drescher
Date: Jul 2, 2021，Views：869
Original protocol used in research article：
L-Selectin/CD62L Is a Key Driver of Non-Alcoholic Steatohepatitis in Mice and Men
Cells, Apr 29, 2020; DOI: 10.3390/cells9051106
Preparation of libraries for yeast ribosome footprints
Authors: Rachel Green and Colin Wu
Date: Jul 2, 2021，Views：874
Original protocol used in research article：
The endonuclease Cue2 cleaves mRNAs at stalled ribosomes during No Go Decay
eLife, Jun 20, 2019; DOI: 10.7554/eLife.49117
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