Protocols shared by authors via RaP service
Journal
Confocal microscopy and image analysis
Authors: Jan N Hansen, Fabian Kaiser, Florian I Schmidt and Dagmar Wachten
Date: Jun 1, 2021，Views：1036
Original protocol used in research article：
Nanobody-directed targeting of optogenetic tools to study signaling in the primary cilium
eLife, Jun 24, 2020; DOI: 10.7554/eLife.57907
PCR amplification and next generation sequencing of sgRNAs
Authors: Xiangbing Qi and Ting Han
Date: May 31, 2021，Views：1088
Original protocol used in research article：
Discovery of a molecular glue promoting CDK12-DDB1 interaction to trigger cyclin K degradation
eLife, Aug 17, 2020; DOI: 10.7554/eLife.59994
Data analysis for genome-wide CRISPR knockout screening
Authors: Xiangbing Qi and Ting Han
Date: May 31, 2021，Views：947
Original protocol used in research article：
Discovery of a molecular glue promoting CDK12-DDB1 interaction to trigger cyclin K degradation
eLife, Aug 17, 2020; DOI: 10.7554/eLife.59994
Co-immunoprecipitation of CCNK and DDB1 with 3xFLAG-CDK12 complex
Authors: Longzhi Cao, Xiangbing Qi and Ting Han
Date: May 31, 2021，Views：978
Original protocol used in research article：
Discovery of a molecular glue promoting CDK12-DDB1 interaction to trigger cyclin K degradation
eLife, Aug 17, 2020; DOI: 10.7554/eLife.59994
Remodeling of the spleen tissue
Authors: Lintao Wang, Junfeng Zhang, Chunming Wang and Lei Dong
Date: May 31, 2021，Views：890
Original protocol used in research article：
Transforming the spleen into a liver-like organ in vivo
Science Advances, Jun 9, 2020; DOI: 10.1126/sciadv.aaz9974
Purification of DDB1ΔBPB
Author: Longzhi Cao
Date: May 31, 2021，Views：1001
Original protocol used in research article：
Discovery of a molecular glue promoting CDK12-DDB1 interaction to trigger cyclin K degradation
eLife, Aug 17, 2020; DOI: 10.7554/eLife.59994
Metabolomic analysis
Authors: Shenglin Luo, Yang Wang and Chunmeng Shi
Date: May 30, 2021，Views：939
Original protocol used in research article：
Near-infrared oxidative phosphorylation inhibitor integrates acute myeloid leukemia–targeted imaging and therapy
Science Advances, Dec 31, 2020; DOI: 10.1126/sciadv.abb6104
IFNγ ELISpot
Author: Christopher M. Haqq
Date: May 27, 2021，Views：1008
Original protocol used in research article：
A lymph node–targeted Amphiphile vaccine induces potent cellular and humoral immunity to SARS-CoV-2
Science Advances, Feb 4, 2021; DOI: 10.1126/sciadv.abe5819
Single hepatocyte collection
Authors: S. Sun and J. Vijg
Date: May 26, 2021，Views：939
Original protocol used in research article：
Single-cell analysis reveals different age-related somatic mutation profiles between stem and differentiated cells in human liver
Science Advances, Jan 30, 2020; DOI: 10.1126/sciadv.aax2659
Quantification of food intake
Authors: Caroline Lei Wee, Sam Kunes and Erin Song
Date: May 26, 2021，Views：1119
Original protocol used in research article：
A bidirectional network for appetite control in larval zebrafish
eLife, Oct 18, 2019; DOI: 10.7554/eLife.43775
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