Protocols shared by authors via RaP service
Journal
Cell preparation and flow cytometry
Author: Niklas K. Björkström
Date: May 24, 2021，Views：906
Original protocol used in research article：
Natural killer cell immunotypes related to COVID-19 disease severity
Science Immunology, Aug 21, 2020; DOI: 10.1126/sciimmunol.abd6832
Histology and Immunohistochemistry
Authors: Yiqiao Hu, Jinhui Wu and Huanhuan Chen
Date: May 23, 2021，Views：880
Original protocol used in research article：
Dissolved oxygen from microalgae-gel patch promotes chronic wound healing in diabetes
Science Advances, May 14, 2020; DOI: 10.1126/sciadv.aba4311
Fabrication of ZIF-8, NV-ZIF, and NV-ZIFMCF
Author: Niveen M. Khashab
Date: May 23, 2021，Views：1014
Original protocol used in research article：
Sustained and targeted delivery of checkpoint inhibitors by metal-organic frameworks for cancer immunotherapy
Science Advances, Jan 21, 2021; DOI: 10.1126/sciadv.abe7174
CC membrane protein characterization
Author: Niveen M. Khashab
Date: May 23, 2021，Views：813
Original protocol used in research article：
Sustained and targeted delivery of checkpoint inhibitors by metal-organic frameworks for cancer immunotherapy
Science Advances, Jan 21, 2021; DOI: 10.1126/sciadv.abe7174
Bacterial Cell Length and Motility Measurement
Authors: Weijie Chen, Sridhar Mani and Jay X Tang
Date: May 22, 2021，Views：1099
Original protocol used in research article：
Confinement discerns swarmers from planktonic bacteria
eLife, Apr 22, 2021; DOI: 10.7554/eLife.64176
Coexpression and cis-motif enrichment analyses
Authors: Sandip M Kale and Ravi Koppolu
Date: May 22, 2021，Views：968
Original protocol used in research article：
Transcriptional landscapes of floral meristems in barley
Science Advances, Apr 28, 2021; DOI: 10.1126/sciadv.abf0832
Immunofluorescence
Authors: Li-Yin Hung and De’Broski R. Herbert
Date: May 21, 2021，Views：917
Original protocol used in research article：
Cellular context of IL-33 expression dictates impact on anti-helminth immunity
Science Immunology, Nov 12, 2020; DOI: 10.1126/sciimmunol.abc6259
Preparation of yeast membranes
Authors: Sisi Lin, Subhajit Mukherjee, Juanjuan Li, Weiliang Hou, Chao Pan and Jinyao Liu
Date: May 21, 2021，Views：979
Original protocol used in research article：
Mucosal immunity–mediated modulation of the gut microbiome by oral delivery of probiotics into Peyer’s patches
Science Advances, May 12, 2021; DOI: 10.1126/sciadv.abf0677
MCAO Stroke Model Protocol for Mouse
Authors: Luke R Lemmerman, Hallie N Harris, W. David Arnold, Natalia Higuita-Castro and Daniel Gallego-Perez
Date: May 21, 2021，Views：1165
Original protocol used in research article：
Nanotransfection-based vasculogenic cell reprogramming drives functional recovery in a mouse model of ischemic stroke
Science Advances, Mar 18, 2021; DOI: 10.1126/sciadv.abd4735
Cell lines
Authors: Zuodong Zhao, Zhuqiang Zhang and Bing Zhu
Date: May 20, 2021，Views：781
Original protocol used in research article：
Sustained TNF-α stimulation leads to transcriptional memory that greatly enhances signal sensitivity and robustness
eLife, Nov 6, 2020; DOI: 10.7554/eLife.61965
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