Bio-101 - Improve Research Reproducibility

Optic fiber implantation

Authors: Mehran Ahmadlou and J. Alexander Heimel
Date: Jun 9, 2021，Views：877

Original protocol used in research article：

A cell type–specific cortico-subcortical brain circuit for investigatory and novelty-seeking behavior
Science, May 14, 2021; DOI: 10.1126/science.abe9681

In vitro immunoregulation of osteogenesis of MSCs

Authors: Xiangmei Liu, Cao Yang and Kelvin W. K. Yeung
Date: Jun 9, 2021，Views：874

Original protocol used in research article：

Regulation of macrophage polarization through surface topography design to facilitate implant-to-bone osteointegration
Science Advances, Apr 1, 2021; DOI: 10.1126/sciadv.abf6654

Label-free quantitative proteomic analysis of enriched phosphopeptides from dark- and light-adapted bovine retinas

Author: Ana Méndez
Date: Jun 8, 2021，Views：902

Original protocol used in research article：

Post-translational regulation of retinal IMPDH1 in vivo to adjust GTP synthesis to illumination conditions
eLife, Apr 7, 2020; DOI: 10.7554/eLife.56418

Machine learning regression

Authors: Xiangang Hu and Fubo Yu
Date: Jun 8, 2021，Views：1019

Original protocol used in research article：

Deep exploration of random forest model boosts the interpretability of machine learning studies of complicated immune responses and lung burden of nanoparticles
Science Advances, May 26, 2021; DOI: 10.1126/sciadv.abf4130

In situ metabolic labeling

Author: Ana Méndez
Date: Jun 8, 2021，Views：871

Original protocol used in research article：

Post-translational regulation of retinal IMPDH1 in vivo to adjust GTP synthesis to illumination conditions
eLife, Apr 7, 2020; DOI: 10.7554/eLife.56418

Preparation of Aβ solution

Author: Peter Niraj Nirmalraj
Date: Jun 7, 2021，Views：920

Original protocol used in research article：

Complete aggregation pathway of amyloid β (1-40) and (1-42) resolved on an atomically clean interface
Science Advances, Apr 7, 2020; DOI: 10.1126/sciadv.aaz6014

Reverse transcription (RT) and quantitative RT-PCR analysis

Authors: Ziling Fang, Shelya X Zeng, Jianping Xiong and Hua Lu
Date: Jun 6, 2021，Views：894

Original protocol used in research article：

SPIN1 promotes tumorigenesis by blocking the uL18 (universal large ribosomal subunit protein 18)-MDM2-p53 pathway in human cancer
eLife, Mar 16, 2018; DOI: 10.7554/eLife.31275

Parallel reaction monitoring MS

Authors: Eric Chiles, Amanda Williams, Debashish Bhattacharya and Xiaoyang Su
Date: Jun 4, 2021，Views：925

Original protocol used in research article：

Metabolomic shifts associated with heat stress in coral holobionts
Science Advances, Dec 31, 2020; DOI: 10.1126/sciadv.abd4210

Wounding Zebrafish Larval Epidermis by Laceration

Authors: Andrew S Kennard, Ellen Labuz, Christopher K Prinz and Julie A Theriot
Date: Jun 3, 2021，Views：1246

Original protocol used in research article：

Osmolarity-independent electrical cues guide rapid response to injury in zebrafish epidermis
eLife, Nov 23, 2020; DOI: 10.7554/eLife.62386

Real-Time PCR

Authors: Ming Xu and James L Kirkland
Date: Jun 3, 2021，Views：1022

Original protocol used in research article：

Targeting senescent cells enhances adipogenesis and metabolic function in old age
eLife, Dec 19, 2015; DOI: 10.7554/eLife.12997

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