Protocols shared by authors via RaP service
Journal
Social Interaction Test
Authors: Cassandra M. Moshfegh and Adam J Case
Date: Jun 17, 2021，Views：1500
Original protocol used in research article：
Autonomic and Redox Imbalance Correlates With T-Lymphocyte Inflammation in a Model of Chronic Social Defeat Stress
Frontiers in Behavioral Neuroscience, May 14, 2019; DOI: 10.3389/fnbeh.2019.00103
Loading and release of siRNA from albumin NPs
Authors: Maria G. Castro and Joerg Lahann
Date: Jun 17, 2021，Views：877
Original protocol used in research article：
Systemic brain tumor delivery of synthetic protein nanoparticles for glioblastoma therapy
Nature Communications, Nov 10, 2020; DOI: 10.1038/s41467-020-19225-7
Heterotopic Cardiac Transplantation
Authors: Reza Motallebzadeh and Gavin J. Pettigrew
Date: Jun 17, 2021，Views：901
Original protocol used in research article：
Relative Frequencies of Alloantigen-Specific Helper CD4 T Cells and B Cells Determine Mode of Antibody-Mediated Allograft Rejection
Frontiers in Immunology, Jan 22, 2019; DOI: 10.3389/fimmu.2018.03039
Establishment of PD-1 Flag-tagged constructs
Authors: Judith Weyershaeuser and Maja Köhn
Date: Jun 16, 2021，Views：886
Original protocol used in research article：
Molecular mechanism of SHP2 activation by PD-1 stimulation
Science Advances, Jan 30, 2020; DOI: 10.1126/sciadv.aay4458
In vitro co-culture and 3D matrigel/collagen culture assays
Author: Don L. Gibbons
Date: Jun 15, 2021，Views：861
Original protocol used in research article：
Collagen promotes anti-PD-1/PD-L1 resistance in cancer through LAIR1-dependent CD8+ T cell exhaustion
Nature Communications, Sep 9, 2020; DOI: 10.1038/s41467-020-18298-8
In vitro microtubule-binding assays
Authors: Jianguo Chen and Junlin Teng
Date: Jun 12, 2021，Views：846
Original protocol used in research article：
CCDC74A/B are K-fiber crosslinkers required for chromosomal alignment
BMC Biology, Sep 14, 2019; DOI: 10.1186/s12915-019-0694-9
Complex I Enzymatic Activity Assay
Author: Shaobai Huang
Date: Jun 11, 2021，Views：838
Original protocol used in research article：
Mitochondrial CLPP2 Assists Coordination and Homeostasis of Respiratory Complexes1,[OPEN]
Plant Physiology, Jun 22, 2020; DOI: 10.1104/pp.20.00136
LacZ activity measurement.
Authors: Zining Wang and Xiaojun Xia
Date: Jun 10, 2021，Views：852
Original protocol used in research article：
cGAS/STING axis mediates a topoisomerase II inhibitor–induced tumor immunogenicity
The Journal of Clinical Investigation, Oct 7, 2019; DOI: 10.1172/JCI127471
Quantification of brain infarct volume
Authors: Weidong Liang and Chunshui Lin
Date: Jun 10, 2021，Views：902
Original protocol used in research article：
Preactivation of Notch1 in remote ischemic preconditioning reduces cerebral ischemia-reperfusion injury through crosstalk with the NF-κB pathway
Journal of Neuroinflammation, Sep 16, 2019; DOI: 10.1186/s12974-019-1570-9
Optic fiber implantation
Authors: Mehran Ahmadlou and J. Alexander Heimel
Date: Jun 9, 2021，Views：877
Original protocol used in research article：
A cell type–specific cortico-subcortical brain circuit for investigatory and novelty-seeking behavior
Science, May 14, 2021; DOI: 10.1126/science.abe9681
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