Bio-101 - Improve Research Reproducibility

FRET-based nucleosomes sliding

Authors: Robert L. Yauch and Tharu Fernando
Date: Jun 24, 2021，Views：760

Original protocol used in research article：

Functional characterization of SMARCA4 variants identified by targeted exome-sequencing of 131,668 cancer patients
Nature Communications, Nov 3, 2020; DOI: 10.1038/s41467-020-19402-8

TRITC-transferrin internalization assay

Author: Feng Liu
Date: Jun 24, 2021，Views：834

Original protocol used in research article：

Rab5c-mediated endocytic trafficking regulates hematopoietic stem and progenitor cell development via Notch and AKT signaling
PLoS Biology, Apr 10, 2020; DOI: 10.1371/journal.pbio.3000696

In vitro MV uptake assay

Authors: Ying Ying Tan, Kieran P. O’Dea and Masao Takata
Date: Jun 24, 2021，Views：984

Original protocol used in research article：

Monocytes mediate homing of circulating microvesicles to the pulmonary vasculature during low-grade systemic inflammation
Journal of Extracellular Vesicles, Jan 5, 2020; DOI: 10.1080/20013078.2019.1706708

Measuring Endogenous Phytohormones

Authors: Yunping Chen, Zhiwu Dan and Shaoqing Li
Date: Jun 24, 2021，Views：1044

Original protocol used in research article：

Rice GROWTH-REGULATING FACTOR7 Modulates Plant Architecture through Regulating GA and Indole-3-Acetic Acid Metabolism1,[OPEN]
Plant Physiology, Jun 24, 2020; DOI: 10.1104/pp.20.00302

Larval Locomotion Assay

Authors: Lilian A. Patron and Konrad E. Zinsmaier
Date: Jun 23, 2021，Views：803

Original protocol used in research article：

Cul4 ubiquitin ligase cofactor DCAF12 promotes neurotransmitter release and homeostatic plasticity
The Journal of Cell Biology, Mar 4, 2019; DOI: 10.1083/jcb.201805099

H&E Staining, Masson Trichrome Staining, and Immunohistochemistry of Kidney Tissues

Authors: Ying-Jan Wang and Rong-Jane Chen
Date: Jun 23, 2021，Views：996

Original protocol used in research article：

Induction of Autophagy by Pterostilbene Contributes to the Prevention of Renal Fibrosis via Attenuating NLRP3 Inflammasome Activation and Epithelial-Mesenchymal Transition
Frontiers in Cell and Developmental Biology, Jan 1, 2020; DOI: 10.3389/fcell.2020.00436

Colon loop ligation assay.

Authors: Liang Tao, Min Dong and jie Zhang
Date: Jun 23, 2021，Views：827

Original protocol used in research article：

Sulfated glycosaminoglycans and low-density lipoprotein receptor contribute to Clostridium difficile toxin A entry into cells
Nat Microbiol, Jun 3, 2019; DOI: 10.1038/s41564-019-0464-z

Sorting of antigen-specific single B cells by flow cytometry

Author: Wei Jiang
Date: Jun 23, 2021，Views：840

Original protocol used in research article：

Systemic translocation of Staphylococcus drives autoantibody production in HIV disease
Microbiome, Feb 14, 2019; DOI: 10.1186/s40168-019-0646-1

Serum-free primary astrocyte culture

Author: Raymundo D Hernandez
Date: Jun 23, 2021，Views：809

Original protocol used in research article：

Astrocyte morphogenesis is dependent on BDNF signaling via astrocytic TrkB.T1
eLife, Aug 21, 2019; DOI: 10.7554/eLife.44667

BV2 Microglial Culture and Microglial Exosome Isolation

Author: Yusheng Wang
Date: Jun 23, 2021，Views：942

Original protocol used in research article：

Exosomes from Microglia Attenuate Photoreceptor Injury and Neovascularization in an Animal Model of Retinopathy of Prematurity
Molecular Therapy. Nucleic Acids, May 17, 2019; DOI: 10.1016/j.omtn.2019.04.029

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