Bio-101 - Improve Research Reproducibility

2.13. Immunofluorescence Staining of the Kidney Sections

Authors: Gry H. Dihazi, Marwa Eltoweissy, Olaf Jahn and Hassan Dihazi
Date: Jul 2, 2021，Views：816

Original protocol used in research article：

The Secretome Analysis of Activated Human Renal Fibroblasts Revealed Beneficial Effect of the Modulation of the Secreted Peptidyl-Prolyl Cis-Trans Isomerase A in Kidney Fibrosis
Cells, Jan 1, 2020; DOI: 10.3390/cells9071724

Enzymatic Method Measurement of Serum Amylase and Lipase

Authors: Shengchuan Chen and Feng Ma
Date: Jul 2, 2021，Views：901

Original protocol used in research article：

TLR3 Ligand PolyI:C Prevents Acute Pancreatitis Through the Interferon-β/Interferon-α/β Receptor Signaling Pathway in a Caerulein-Induced Pancreatitis Mouse Model
Frontiers in Immunology, May 3, 2019; DOI: 10.3389/fimmu.2019.00980

Time-lapse live imaging.

Authors: Allon M. Klein and Ophir D. Klein
Date: Jul 1, 2021，Views：791

Original protocol used in research article：

A large pool of actively cycling progenitors orchestrates self-renewal and injury repair of an ectodermal appendage
Nat Cell Biol, Sep 2, 2019; DOI: 10.1038/s41556-019-0378-2

In vitro kinase assays for STPKs.

Author: Ramandeep Singh
Date: Jun 30, 2021，Views：791

Original protocol used in research article：

NU-6027 Inhibits Growth of Mycobacterium tuberculosis by Targeting Protein Kinase D and Protein Kinase G
Antimicrobial Agents and Chemotherapy, Aug 23, 2019; DOI: 10.1128/AAC.00996-19

2.5. Cell proliferation assay

Authors: Zhixiong Cai, Xiaolong Liu and Jingfeng Liu
Date: Jun 30, 2021，Views：1105

Original protocol used in research article：

Circular RNA profiling identifies circADAMTS13 as a miR‐484 sponge which suppresses cell proliferation in hepatocellular carcinoma
Molecular Oncology, Jan 9, 2019; DOI: 10.1002/1878-0261.12424

Mixed bone marrow and mixed T cell adoptive transfer

Authors: Shao-Cong Sun and xiaofei zhou
Date: Jun 28, 2021，Views：820

Original protocol used in research article：

The deubiquitinase Otub1 controls the activation of CD8 T cells and NK cells by regulating IL-15-mediated priming
Nat Immunol, Jun 10, 2019; DOI: 10.1038/s41590-019-0405-2

SFB colonization

Authors: Daria Esterházy and Daniel Mucida
Date: Jun 28, 2021，Views：869

Original protocol used in research article：

Compartmentalized gut lymph node drainage dictates adaptive immune responses
Nature, Apr 15, 2019; DOI: 10.1038/s41586-019-1125-3

Membrane protein detection

Authors: Shao-Cong Sun and Xiaofei Zhou
Date: Jun 28, 2021，Views：844

Original protocol used in research article：

The deubiquitinase Otub1 controls the activation of CD8 T cells and NK cells by regulating IL-15-mediated priming
Nat Immunol, Jun 10, 2019; DOI: 10.1038/s41590-019-0405-2

FluoroSpot Assay for ASCs

Authors: Renata Varnaitė and Sara Gredmark-Russ
Date: Jun 28, 2021，Views：830

Original protocol used in research article：

Expansion of SARS-CoV-2–Specific Antibody-Secreting Cells and Generation of Neutralizing Antibodies in Hospitalized COVID-19 Patients
J Immunol, Sep 2, 2020; DOI: 10.4049/jimmunol.2000717

In vitro Suppression Assay and Transmigration Assay

Authors: Ivan Koprivica and Ivana Stojanović
Date: Jun 28, 2021，Views：851

Original protocol used in research article：

Ethyl Pyruvate Stimulates Regulatory T Cells and Ameliorates Type 1 Diabetes Development in Mice
Frontiers in Immunology, Jan 10, 2019; DOI: 10.3389/fimmu.2018.03130

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