Bio-101 - Improve Research Reproducibility

CRISPR/Cas9 PRMT1 knockout

Authors: María F. Montenegro and José Neptuno Rodríguez-López
Date: Jun 23, 2021，Views：805

Original protocol used in research article：

PRMT1-dependent methylation of BRCA1 contributes to the epigenetic defense of breast cancer cells against ionizing radiation
Scientific Reports, Jan 1, 2020; DOI: 10.1038/s41598-020-70289-3

Multistep resistance generation evaluation

Authors: Silei Bai, Jianxue Wang, Yugang Bai and Xinxin Feng
Date: Jun 22, 2021，Views：837

Original protocol used in research article：

A polymeric approach toward resistance-resistant antimicrobial agent with dual-selective mechanisms of action
Science Advances, Jan 27, 2021; DOI: 10.1126/sciadv.abc9917

Assays of cellular stress.

Authors: Olumuyiwa Igbalajobi, Zhenzhong Yu and Reinhard Fischer
Date: Jun 22, 2021，Views：802

Original protocol used in research article：

Red- and Blue-Light Sensing in the Plant Pathogen Alternaria alternata Depends on Phytochrome and the White-Collar Protein LreA
mBio, Apr 9, 2019; DOI: 10.1128/mBio.00371-19

Xenograft in zebrafish

Author: Ji Eun Lee
Date: Jun 19, 2021，Views：798

Original protocol used in research article：

CEP41‐mediated ciliary tubulin glutamylation drives angiogenesis through AURKA‐dependent deciliation
EMBO Reports, Dec 29, 2019; DOI: 10.15252/embr.201948290

Expression and purification of recombinant SARS-CoV-2 Spike protein

Authors: Jun Zhang, Yongfei Cai and Bing Chen
Date: Jun 18, 2021，Views：1088

Original protocol used in research article：

Structural impact on SARS-CoV-2 spike protein by D614G substitution
Science, Apr 30, 2021; DOI: 10.1126/science.abf2303

Glutamate clearance assay.

Authors: Venkata Viswanadh Edara and Kathleen Borgmann
Date: Jun 18, 2021，Views：781

Original protocol used in research article：

Insights into the Gene Expression Profiles of Active and Restricted Red/Green-HIV+ Human Astrocytes: Implications for Shock or Lock Therapies in the Brain
Journal of Virology, Feb 28, 2020; DOI: 10.1128/JVI.01563-19

Social Interaction Test

Authors: Cassandra M. Moshfegh and Adam J Case
Date: Jun 17, 2021，Views：1133

Original protocol used in research article：

Autonomic and Redox Imbalance Correlates With T-Lymphocyte Inflammation in a Model of Chronic Social Defeat Stress
Frontiers in Behavioral Neuroscience, May 14, 2019; DOI: 10.3389/fnbeh.2019.00103

Loading and release of siRNA from albumin NPs

Authors: Maria G. Castro and Joerg Lahann
Date: Jun 17, 2021，Views：839

Original protocol used in research article：

Systemic brain tumor delivery of synthetic protein nanoparticles for glioblastoma therapy
Nature Communications, Nov 10, 2020; DOI: 10.1038/s41467-020-19225-7

Heterotopic Cardiac Transplantation

Authors: Reza Motallebzadeh and Gavin J. Pettigrew
Date: Jun 17, 2021，Views：873

Original protocol used in research article：

Relative Frequencies of Alloantigen-Specific Helper CD4 T Cells and B Cells Determine Mode of Antibody-Mediated Allograft Rejection
Frontiers in Immunology, Jan 22, 2019; DOI: 10.3389/fimmu.2018.03039

Establishment of PD-1 Flag-tagged constructs

Authors: Judith Weyershaeuser and Maja Köhn
Date: Jun 16, 2021，Views：846

Original protocol used in research article：

Molecular mechanism of SHP2 activation by PD-1 stimulation
Science Advances, Jan 30, 2020; DOI: 10.1126/sciadv.aay4458

First
Prev

Next
Last

Protocols 

Request a Protocol

Receive Our Alerts

Submit 

About