Bio-101 - Improve Research Reproducibility

Generation of In vitro Derived Mucus

Authors: Lisa H. Cazares and Sonja Hess
Date: Jun 25, 2021，Views：781

Original protocol used in research article：

Development of a Parallel Reaction Monitoring Mass Spectrometry Assay for the Detection of SARS-CoV-2 Spike Glycoprotein and Nucleoprotein
Anal Chem, Sep 23, 2020; DOI: 10.1021/acs.analchem.0c02288

Linearity of the assay

Author: Nicolas Chomont
Date: Jun 25, 2021，Views：768

Original protocol used in research article：

Single-cell characterization and quantification of translation-competent viral reservoirs in treated and untreated HIV infection
PLoS Pathogens, Feb 27, 2019; DOI: 10.1371/journal.ppat.1007619

Isolation, culture and flow cytometry of bone marrow B cell progenitors.

Authors: Malay Mandal and Marcus R Clark
Date: Jun 24, 2021，Views：734

Original protocol used in research article：

CXCR4 signaling directs Igk recombination and the molecular mechanisms of late B lymphopoiesis
Nat Immunol, Sep 2, 2019; DOI: 10.1038/s41590-019-0468-0

FRET-based nucleosomes sliding

Authors: Robert L. Yauch and Tharu Fernando
Date: Jun 24, 2021，Views：748

Original protocol used in research article：

Functional characterization of SMARCA4 variants identified by targeted exome-sequencing of 131,668 cancer patients
Nature Communications, Nov 3, 2020; DOI: 10.1038/s41467-020-19402-8

TRITC-transferrin internalization assay

Author: Feng Liu
Date: Jun 24, 2021，Views：822

Original protocol used in research article：

Rab5c-mediated endocytic trafficking regulates hematopoietic stem and progenitor cell development via Notch and AKT signaling
PLoS Biology, Apr 10, 2020; DOI: 10.1371/journal.pbio.3000696

In vitro MV uptake assay

Authors: Ying Ying Tan, Kieran P. O’Dea and Masao Takata
Date: Jun 24, 2021，Views：974

Original protocol used in research article：

Monocytes mediate homing of circulating microvesicles to the pulmonary vasculature during low-grade systemic inflammation
Journal of Extracellular Vesicles, Jan 5, 2020; DOI: 10.1080/20013078.2019.1706708

Measuring Endogenous Phytohormones

Authors: Yunping Chen, Zhiwu Dan and Shaoqing Li
Date: Jun 24, 2021，Views：1017

Original protocol used in research article：

Rice GROWTH-REGULATING FACTOR7 Modulates Plant Architecture through Regulating GA and Indole-3-Acetic Acid Metabolism1,[OPEN]
Plant Physiology, Jun 24, 2020; DOI: 10.1104/pp.20.00302

Larval Locomotion Assay

Authors: Lilian A. Patron and Konrad E. Zinsmaier
Date: Jun 23, 2021，Views：788

Original protocol used in research article：

Cul4 ubiquitin ligase cofactor DCAF12 promotes neurotransmitter release and homeostatic plasticity
The Journal of Cell Biology, Mar 4, 2019; DOI: 10.1083/jcb.201805099

H&E Staining, Masson Trichrome Staining, and Immunohistochemistry of Kidney Tissues

Authors: Ying-Jan Wang and Rong-Jane Chen
Date: Jun 23, 2021，Views：972

Original protocol used in research article：

Induction of Autophagy by Pterostilbene Contributes to the Prevention of Renal Fibrosis via Attenuating NLRP3 Inflammasome Activation and Epithelial-Mesenchymal Transition
Frontiers in Cell and Developmental Biology, Jan 1, 2020; DOI: 10.3389/fcell.2020.00436

Colon loop ligation assay.

Authors: Liang Tao, Min Dong and jie Zhang
Date: Jun 23, 2021，Views：812

Original protocol used in research article：

Sulfated glycosaminoglycans and low-density lipoprotein receptor contribute to Clostridium difficile toxin A entry into cells
Nat Microbiol, Jun 3, 2019; DOI: 10.1038/s41564-019-0464-z

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