Bio-101 - Improve Research Reproducibility

SILAC labeling and DNA pull-down experiments

Authors: Reuben S Harris and Dennis Kappei
Date: Jun 23, 2021，Views：942

Original protocol used in research article：

Characterization of the mechanism by which the RB/E2F pathway controls expression of the cancer genomic DNA deaminase APOBEC3B
eLife, Sep 28, 2020; DOI: 10.7554/eLife.61287

In vitro BBB penetration of NPs

Authors: Nitin Joshi and Sezin Aday
Date: Jun 23, 2021，Views：1081

Original protocol used in research article：

BBB pathophysiology–independent delivery of siRNA in traumatic brain injury
Science Advances, Dec 31, 2020; DOI: 10.1126/sciadv.abd6889

2.7. In Vivo Wounding Assay

Author: Vladimir Krylov
Date: Jun 23, 2021，Views：817

Original protocol used in research article：

Epithelial-Mesenchymal Transition Promotes the Differentiation Potential of Xenopus tropicalis Immature Sertoli Cells
Stem Cells International, May 5, 2019; DOI: 10.1155/2019/8387478

ATG5 siRNA Transfection

Author: Anna-Mart Engelbrecht
Date: Jun 23, 2021，Views：847

Original protocol used in research article：

Amino Acid Starvation Sensitizes Resistant Breast Cancer to Doxorubicin-Induced Cell Death
Frontiers in Cell and Developmental Biology, Oct 15, 2020; DOI: 10.3389/fcell.2020.565915

CRISPR/Cas9 PRMT1 knockout

Authors: María F. Montenegro and José Neptuno Rodríguez-López
Date: Jun 23, 2021，Views：833

Original protocol used in research article：

PRMT1-dependent methylation of BRCA1 contributes to the epigenetic defense of breast cancer cells against ionizing radiation
Scientific Reports, Jan 1, 2020; DOI: 10.1038/s41598-020-70289-3

Multistep resistance generation evaluation

Authors: Silei Bai, Jianxue Wang, Yugang Bai and Xinxin Feng
Date: Jun 22, 2021，Views：871

Original protocol used in research article：

A polymeric approach toward resistance-resistant antimicrobial agent with dual-selective mechanisms of action
Science Advances, Jan 27, 2021; DOI: 10.1126/sciadv.abc9917

Assays of cellular stress.

Authors: Olumuyiwa Igbalajobi, Zhenzhong Yu and Reinhard Fischer
Date: Jun 22, 2021，Views：824

Original protocol used in research article：

Red- and Blue-Light Sensing in the Plant Pathogen Alternaria alternata Depends on Phytochrome and the White-Collar Protein LreA
mBio, Apr 9, 2019; DOI: 10.1128/mBio.00371-19

Xenograft in zebrafish

Author: Ji Eun Lee
Date: Jun 19, 2021，Views：832

Original protocol used in research article：

CEP41‐mediated ciliary tubulin glutamylation drives angiogenesis through AURKA‐dependent deciliation
EMBO Reports, Dec 29, 2019; DOI: 10.15252/embr.201948290

Expression and purification of recombinant SARS-CoV-2 Spike protein

Authors: Jun Zhang, Yongfei Cai and Bing Chen
Date: Jun 18, 2021，Views：1197

Original protocol used in research article：

Structural impact on SARS-CoV-2 spike protein by D614G substitution
Science, Apr 30, 2021; DOI: 10.1126/science.abf2303

Glutamate clearance assay.

Authors: Venkata Viswanadh Edara and Kathleen Borgmann
Date: Jun 18, 2021，Views：818

Original protocol used in research article：

Insights into the Gene Expression Profiles of Active and Restricted Red/Green-HIV+ Human Astrocytes: Implications for Shock or Lock Therapies in the Brain
Journal of Virology, Feb 28, 2020; DOI: 10.1128/JVI.01563-19

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