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Biotinylation of Smc3p-AviTag reporters

SX Siheng Xiang
DK Douglas Koshland

Last updated date: Apr 21, 2021 Views: 891 Forks: 0

original An abbreviated version of this protocol was published in eLife in Feb, 2021
Cohesin architecture and clustering in vivo
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The detail protocol was provided in the manuscript, in the section "Biotinylation of Smc3p-AviTag reporters". The reaction volume is 0.75 ml, so 0.25 ml of 80% TCA is added to the reaction to yield a 20% TCA. The recipe of culture media is listed in the section "Yeast strains and media", which has no TCA in it.

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How to cite：
Readers should cite both the Bio-protocol preprint and the original research article where this protocol was used:
  1. Xiang, S and Koshland, D(2021). Biotinylation of Smc3p-AviTag reporters. Bio-protocol Preprint. bio-protocol.org/prep1027.
  2. Xiang, S. and Koshland, D.(2021). Cohesin architecture and clustering in vivo. eLife. DOI: 10.7554/eLife.62243

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