Protocols shared by authors via RaP service
Journal
In vitro permeability assay
Authors: Shima Ghoroghi, Jacky G Goetz and Vincent Hyenne
Date: May 6, 2021，Views：1265
Original protocol used in research article：
Ral GTPases promote breast cancer metastasis by controlling biogenesis and organ targeting of exosomes
eLife, Jan 6, 2021; DOI: 10.7554/eLife.61539
IP and MS
Author: Xiaoming Zhang
Date: May 6, 2021，Views：948
Original protocol used in research article：
DEAD-box helicases modulate dicing body formation in Arabidopsis
Science Advances, Apr 28, 2021; DOI: 10.1126/sciadv.abc6266
Single-cell tissue preparation
Authors: Kristen L Wells, Mark S Anderson, Lars M Steinmetz and Corey N Miller
Date: May 4, 2021，Views：892
Original protocol used in research article：
Combined transient ablation and single-cell RNA-sequencing reveals the development of medullary thymic epithelial cells
eLife, Nov 23, 2020; DOI: 10.7554/eLife.60188
Animal husbandry and handling
Authors: Laura A Lavery and Huda Y Zoghbi
Date: May 4, 2021，Views：848
Original protocol used in research article：
Losing Dnmt3a dependent methylation in inhibitory neurons impairs neural function by a mechanism impacting Rett syndrome
eLife, Mar 11, 2020; DOI: 10.7554/eLife.52981
Integrative RNA-seq and methylation analysis
Authors: Alexander Trostle, Laura A Lavery, Ying-wooi Wan and Huda Y Zoghbi
Date: May 4, 2021，Views：839
Original protocol used in research article：
Losing Dnmt3a dependent methylation in inhibitory neurons impairs neural function by a mechanism impacting Rett syndrome
eLife, Mar 11, 2020; DOI: 10.7554/eLife.52981
Animal experiments
Authors: Hyungsoo Kim and Ze’ev A. Ronai
Date: May 3, 2021，Views：888
Original protocol used in research article：
PRMT5 control of cGAS/STING and NLRC5 pathways defines melanoma response to antitumor immunity
Science Translational Medicine, Jul 7, 2020; DOI: 10.1126/scitranslmed.aaz5683
Real-time place preference – RTPP
Authors: Bárbara Coimbra and Ana João Rodrigues
Date: Apr 29, 2021，Views：938
Original protocol used in research article：
Impairments in laterodorsal tegmentum to VTA projections underlie glucocorticoid-triggered reward deficits
eLife, Aug 24, 2017; DOI: 10.7554/eLife.25843
Binding energy calculations by molecular mechanics–generalized Born surface area
Authors: D. Collias, R. T. Leenay, R. A. Slotkowski, Z. Zuo, J. Liu and C. L. Beisel
Date: Apr 29, 2021，Views：933
Original protocol used in research article：
A positive growth-based PAM screen identifies noncanonical motifs recognized by the S. pyogenes Cas9
Science Advances, Jul 14, 2020; DOI: 10.1126/sciadv.abb4054
Dual RNA-seq sample preparation
Authors: Gili Rosenberg and Roi Avraham
Date: Apr 29, 2021，Views：925
Original protocol used in research article：
Host succinate is an activation signal for Salmonella virulence during intracellular infection
Science, Jan 22, 2021; DOI: 10.1126/science.aba8026
Whisker and other hair trimming
Authors: Kyle S Severson and Daniel H O'Connor
Date: Apr 28, 2021，Views：832
Original protocol used in research article：
Coding of whisker motion across the mouse face
eLife, Feb 28, 2019; DOI: 10.7554/eLife.41535
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