Protocols shared by authors via RaP service
Journal
Modeling Study design
Author: Sixue Zhang
Date: May 19, 2020，Views：904
Original protocol used in research article：
β-amyloid redirects norepinephrine signaling to activate the pathogenic GSK3β/tau cascade
Science Translational Medicine, Jan 14, 2020; DOI: 10.1126/scitranslmed.aay6931
DNA plasmids and cell lines
Authors: Jason E. Lee and Gia K. Voeltz
Date: May 18, 2020，Views：992
Original protocol used in research article：
Endoplasmic reticulum contact sites regulate the dynamics of membraneless organelles
Science, Jan 30, 2020; DOI: 10.1126/science.aay7108
Optimization of planarian fixation for RNA extraction and histology
Author: David J Forsthoefel
Date: May 18, 2020，Views：1121
Original protocol used in research article：
Cell-type diversity and regionalized gene expression in the planarian intestine
eLife, Apr 2, 2020; DOI: 10.7554/eLife.52613
Circadian chemical screen assays with human cell lines and mice locomotor assays
Authors: Eric Erquan Zhang and Dapeng Ju
Date: May 15, 2020，Views：970
Original protocol used in research article：
Chemical perturbations reveal that RUVBL2 regulates the circadian phase in mammals
Science Translational Medicine, May 5, 2020; DOI: 10.1126/scitranslmed.aba0769
Heat-shock microscope and two-photon fluorescent thermometry
Author: Sicong He
Date: May 15, 2020，Views：1176
Original protocol used in research article：
In vivo single-cell lineage tracing in zebrafish using high-resolution infrared laser-mediated gene induction microscopy
eLife, Jan 6, 2020; DOI: 10.7554/eLife.52024
Data analysis with seurat
Author: Aaron M Allen
Date: May 15, 2020，Views：1150
Original protocol used in research article：
A single-cell transcriptomic atlas of the adult Drosophila ventral nerve cord
eLife, Apr 21, 2020; DOI: 10.7554/eLife.54074
FLAG affinity purification and mass spectrometry
Authors: Payal Jain, Cecilia Julia Ballaré and Luciano Di Croce
Date: May 11, 2020，Views：1114
Original protocol used in research article：
PHF19 mediated regulation of proliferation and invasiveness in prostate cancer cells
eLife, Mar 10, 2020; DOI: 10.7554/eLife.51373
Integration site mapping by high-throughput sequencing of Tol2-oligo pulldown-enriched fragments
Authors: Ashwin A Bhandiwad, Tianwei Li, James R Iben, Steven L Coon and Harold A Burgess
Date: May 11, 2020，Views：1316
Original protocol used in research article：
Brain-wide cellular resolution imaging of Cre transgenic zebrafish lines for functional circuit-mapping
eLife, Feb 8, 2019; DOI: 10.7554/eLife.42687
Ligand-receptor networks
Author: Ralph Patrick
Date: May 10, 2020，Views：1208
Original protocol used in research article：
Single-cell expression profiling reveals dynamic flux of cardiac stromal, vascular and immune cells in health and injury
eLife, Mar 26, 2019; DOI: 10.7554/eLife.43882
RT-PCR and quantitative analysis
Author: Emilie Barruet
Date: May 8, 2020，Views：1096
Original protocol used in research article：
Functionally heterogeneous human satellite cells identified by single cell RNA sequencing
eLife, Apr 1, 2020; DOI: 10.7554/eLife.51576
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