Protocols shared by authors via RaP service
Journal
Genus-level phylogenetic tree
Author: Ricardo A. Segovia
Date: May 22, 2020，Views：1106
Original protocol used in research article：
Freezing and water availability structure the evolutionary diversity of trees across the Americas
Science Advances, May 5, 2020; DOI: 10.1126/sciadv.aaz5373
AcrIIA11 homolog discovery and phylogenetic placement
Author: Kevin J Forsberg
Date: May 21, 2020，Views：1190
Original protocol used in research article：
Functional metagenomics-guided discovery of potent Cas9 inhibitors in the human microbiome
eLife, Sep 10, 2019; DOI: 10.7554/eLife.46540
Synthesis of compounds
Author: Samuel T. Jones
Date: May 20, 2020，Views：1007
Original protocol used in research article：
Modified cyclodextrins as broad-spectrum antivirals
Science Advances, Jan 28, 2020; DOI: 10.1126/sciadv.aax9318
Golgi preparations
Authors: Alun M Davies and Paulina Carriba
Date: May 20, 2020，Views：1101
Original protocol used in research article：
CD40 is a major regulator of dendrite growth from developing excitatory and inhibitory neurons
eLife, Nov 7, 2017; DOI: 10.7554/eLife.30442
Modeling Study design
Author: Sixue Zhang
Date: May 19, 2020，Views：946
Original protocol used in research article：
β-amyloid redirects norepinephrine signaling to activate the pathogenic GSK3β/tau cascade
Science Translational Medicine, Jan 14, 2020; DOI: 10.1126/scitranslmed.aay6931
DNA plasmids and cell lines
Authors: Jason E. Lee and Gia K. Voeltz
Date: May 18, 2020，Views：1018
Original protocol used in research article：
Endoplasmic reticulum contact sites regulate the dynamics of membraneless organelles
Science, Jan 30, 2020; DOI: 10.1126/science.aay7108
Optimization of planarian fixation for RNA extraction and histology
Author: David J Forsthoefel
Date: May 18, 2020，Views：1196
Original protocol used in research article：
Cell-type diversity and regionalized gene expression in the planarian intestine
eLife, Apr 2, 2020; DOI: 10.7554/eLife.52613
Circadian chemical screen assays with human cell lines and mice locomotor assays
Authors: Eric Erquan Zhang and Dapeng Ju
Date: May 15, 2020，Views：1010
Original protocol used in research article：
Chemical perturbations reveal that RUVBL2 regulates the circadian phase in mammals
Science Translational Medicine, May 5, 2020; DOI: 10.1126/scitranslmed.aba0769
Heat-shock microscope and two-photon fluorescent thermometry
Author: Sicong He
Date: May 15, 2020，Views：1208
Original protocol used in research article：
In vivo single-cell lineage tracing in zebrafish using high-resolution infrared laser-mediated gene induction microscopy
eLife, Jan 6, 2020; DOI: 10.7554/eLife.52024
Data analysis with seurat
Author: Aaron M Allen
Date: May 15, 2020，Views：1200
Original protocol used in research article：
A single-cell transcriptomic atlas of the adult Drosophila ventral nerve cord
eLife, Apr 21, 2020; DOI: 10.7554/eLife.54074
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