Protocols shared by authors via RaP service
Journal
Primary bone marrow stromal cultures
Author: Elie Traer
Date: Jul 2, 2020，Views：1017
Original protocol used in research article：
FGF2-FGFR1 signaling regulates release of Leukemia-Protective exosomes from bone marrow stromal cells
eLife, Feb 5, 2019; DOI: 10.7554/eLife.40033
In vitro B cell stimulation
Authors: Julie Zikherman and James L. Mueller
Date: Jul 2, 2020，Views：1911
Original protocol used in research article：
IgM and IgD B cell receptors differentially respond to endogenous antigens and control B cell fate
eLife, Mar 9, 2018; DOI: 10.7554/eLife.35074
Real-time qRT-PCR analysis
Authors: Ane Martin Anduaga and Sebastian Kadener
Date: Jul 2, 2020，Views：1347
Original protocol used in research article：
Thermosensitive alternative splicing senses and mediates temperature adaptation in Drosophila
eLife, Nov 8, 2019; DOI: 10.7554/eLife.44642
RNA FISH
Author: Xiling Shen
Date: Jul 1, 2020，Views：1121
Original protocol used in research article：
A long non-coding RNA targets microRNA miR-34a to regulate colon cancer stem cell asymmetric division
eLife, Apr 14, 2016; DOI: 10.7554/eLife.14620
ECG recordings
Authors: Wenli Dai and Christopher R Weber
Date: Jul 1, 2020，Views：993
Original protocol used in research article：
A calcium transport mechanism for atrial fibrillation in Tbx5-mutant mice
eLife, Mar 21, 2019; DOI: 10.7554/eLife.41814
Identification of pause events
Author: Nir Fluman
Date: Jul 1, 2020，Views：1035
Original protocol used in research article：
mRNA-programmed translation pauses in the targeting of E. coli membrane proteins
eLife, Aug 18, 2014; DOI: 10.7554/eLife.03440
3D reconstruction of SGs
Authors: SeYeon Chung and Deborah J Andrew
Date: Jul 1, 2020，Views：973
Original protocol used in research article：
Uncoupling apical constriction from tissue invagination
eLife, Mar 6, 2017; DOI: 10.7554/eLife.22235
Virus-like particles (VLPs)
Authors: LUKE CHAO, Adam Johnson and Stephen C Harrison
Date: Jul 1, 2020，Views：1791
Original protocol used in research article：
How small-molecule inhibitors of dengue-virus infection interfere with viral membrane fusion
eLife, Jul 12, 2018; DOI: 10.7554/eLife.36461
Virus-like particles (VLPs)
Authors: LUKE CHAO, Adam Johnson and Stephen C Harrison
Date: Jul 1, 2020，Views：1791
Original protocol used in research article：
Sequential conformational rearrangements in flavivirus membrane fusion
eLife, Dec 5, 2014; DOI: 10.7554/eLife.04389
Detection of nucleosome positions
Authors: Erin K O'Shea and Xu Zhou
Date: Jul 1, 2020，Views：1011
Original protocol used in research article：
A computational approach to map nucleosome positions and alternative chromatin states with base pair resolution
eLife, Sep 13, 2016; DOI: 10.7554/eLife.16970
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