Protocols shared by authors via RaP service
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Circadian chemical screen assays with human cell lines and mice locomotor assays
Authors: Eric Erquan Zhang and Dapeng Ju
Date: May 15, 2020，Views：989
Original protocol used in research article：
Chemical perturbations reveal that RUVBL2 regulates the circadian phase in mammals
Science Translational Medicine, May 5, 2020; DOI: 10.1126/scitranslmed.aba0769
Heat-shock microscope and two-photon fluorescent thermometry
Author: Sicong He
Date: May 15, 2020，Views：1199
Original protocol used in research article：
In vivo single-cell lineage tracing in zebrafish using high-resolution infrared laser-mediated gene induction microscopy
eLife, Jan 6, 2020; DOI: 10.7554/eLife.52024
Data analysis with seurat
Author: Aaron M Allen
Date: May 15, 2020，Views：1171
Original protocol used in research article：
A single-cell transcriptomic atlas of the adult Drosophila ventral nerve cord
eLife, Apr 21, 2020; DOI: 10.7554/eLife.54074
FLAG affinity purification and mass spectrometry
Authors: Payal Jain, Cecilia Julia Ballaré and Luciano Di Croce
Date: May 11, 2020，Views：1145
Original protocol used in research article：
PHF19 mediated regulation of proliferation and invasiveness in prostate cancer cells
eLife, Mar 10, 2020; DOI: 10.7554/eLife.51373
Integration site mapping by high-throughput sequencing of Tol2-oligo pulldown-enriched fragments
Authors: Ashwin A Bhandiwad, Tianwei Li, James R Iben, Steven L Coon and Harold A Burgess
Date: May 11, 2020，Views：1336
Original protocol used in research article：
Brain-wide cellular resolution imaging of Cre transgenic zebrafish lines for functional circuit-mapping
eLife, Feb 8, 2019; DOI: 10.7554/eLife.42687
Ligand-receptor networks
Author: Ralph Patrick
Date: May 10, 2020，Views：1227
Original protocol used in research article：
Single-cell expression profiling reveals dynamic flux of cardiac stromal, vascular and immune cells in health and injury
eLife, Mar 26, 2019; DOI: 10.7554/eLife.43882
RT-PCR and quantitative analysis
Author: Emilie Barruet
Date: May 8, 2020，Views：1130
Original protocol used in research article：
Functionally heterogeneous human satellite cells identified by single cell RNA sequencing
eLife, Apr 1, 2020; DOI: 10.7554/eLife.51576
STAG1-AID , STAG2-AID
Author: Wen Tang
Date: May 8, 2020，Views：1143
Original protocol used in research article：
ESCO1 and CTCF enable formation of long chromatin loops by protecting cohesinSTAG1 from WAPL
eLife, Feb 17, 2020; DOI: 10.7554/eLife.52091
Cell lysis
Author: Byoungsan Choi
Date: May 8, 2020，Views：2178
Original protocol used in research article：
Single-molecule functional anatomy of endogenous HER2-HER3 heterodimers
eLife, Apr 8, 2020; DOI: 10.7554/eLife.53934
Sample size calculations and statistical analyses
Author: Asuncion Mejias
Date: May 7, 2020，Views：945
Original protocol used in research article：
Immune profiles provide insights into respiratory syncytial virus disease severity in young children
Science Translational Medicine, Apr 21, 2020; DOI: 10.1126/scitranslmed.aaw0268
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