Protocols shared by authors via RaP service
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Bioprinting of vascular GBM model
Authors: Xavier Intes, Guohao Dai, Mehmet S. Ozturk and Vivian K. Lee
Date: May 4, 2020，Views：923
Original protocol used in research article：
High-resolution tomographic analysis of in vitro 3D glioblastoma tumor model under long-term drug treatment
Science Advances, Mar 5, 2020; DOI: 10.1126/sciadv.aay7513
Quantification of plasma bile acids and bilirubin
Authors: Huasheng Yu, Wenqin Luo and Yulong Li
Date: May 3, 2020，Views：1305
Original protocol used in research article：
MRGPRX4 is a bile acid receptor for human cholestatic itch
eLife, Sep 10, 2019; DOI: 10.7554/eLife.48431
MG132 treatment of worms
Author: Ying Liu
Date: May 2, 2020，Views：1123
Original protocol used in research article：
SUMO peptidase ULP-4 regulates mitochondrial UPR-mediated innate immunity and lifespan extension
eLife, Jan 15, 2019; DOI: 10.7554/eLife.41792
Model equations
Author: Clara Champagne
Date: May 2, 2020，Views：1103
Original protocol used in research article：
Structure in the variability of the basic reproductive number (R0) for Zika epidemics in the Pacific islands
eLife, Nov 29, 2016; DOI: 10.7554/eLife.19874
Tissue preparation, immunohistochemistry and in situ hybridization
Authors: Jane E Johnson, Juan Villarreal and Trisha Savage
Date: May 1, 2020，Views：2134
Original protocol used in research article：
Repression by PRDM13 is critical for generating precision in neuronal identity
eLife, Aug 29, 2017; DOI: 10.7554/eLife.25787
Creoptix WAVE experiments
Author: Gunther Kern
Date: Apr 30, 2020，Views：1225
Original protocol used in research article：
Dynamics of human protein kinase Aurora A linked to drug selectivity
eLife, Jun 14, 2018; DOI: 10.7554/eLife.36656
Real-time PCR analysis
Author: Natascha Ellen Stumpf
Date: Apr 30, 2020，Views：1025
Original protocol used in research article：
A high-salt diet compromises antibacterial neutrophil responses through hormonal perturbation
Science Translational Medicine, Mar 24, 2020; DOI: 10.1126/scitranslmed.aay3850
Cell fractionation
Authors: Cecilia Julia Ballaré and Luciano Di Croce
Date: Apr 29, 2020，Views：1050
Original protocol used in research article：
PHF19 mediated regulation of proliferation and invasiveness in prostate cancer cells
eLife, Mar 10, 2020; DOI: 10.7554/eLife.51373
ATAC-Seq
Authors: Sarah E Dremel and Neal A DeLuca
Date: Apr 28, 2020，Views：5927
Original protocol used in research article：
Herpes simplex viral nucleoprotein creates a competitive transcriptional environment facilitating robust viral transcription and host shut off
eLife, Oct 22, 2019; DOI: 10.7554/eLife.51109
Concanavalin A enrichment
Authors: Matthew Freeman and Adam G Grieve
Date: Apr 28, 2020，Views：1439
Original protocol used in research article：
FRMD8 promotes inflammatory and growth factor signalling by stabilising the iRhom/ADAM17 sheddase complex
eLife, Jun 13, 2018; DOI: 10.7554/eLife.35012
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