Protocols shared by authors via RaP service
Journal
X34 staining
Author: Della C David
Date: May 26, 2020，Views：2401
Original protocol used in research article：
Intrinsically aggregation-prone proteins form amyloid-like aggregates and contribute to tissue aging in Caenorhabditis elegans
eLife, May 3, 2019; DOI: 10.7554/eLife.43059
Protein network analysis
Author: Miguel A. Gonzalez-Lozano
Date: May 25, 2020，Views：934
Original protocol used in research article：
Stitching the synapse: Cross-linking mass spectrometry into resolving synaptic protein interactions
Science Advances, Feb 19, 2020; DOI: 10.1126/sciadv.aax5783
Cell viability assays following treatment with aDT–eIF-3b
Author: Laura Smith
Date: May 24, 2020，Views：929
Original protocol used in research article：
Attenuated diphtheria toxin mediates siRNA delivery
Science Advances, Apr 30, 2020; DOI: 10.1126/sciadv.aaz4848
Tau expression and purification
Authors: Wenjuan Zhang, Michel Goedert and Sjors HW Scheres
Date: May 24, 2020，Views：6325
Original protocol used in research article：
Heparin-induced tau filaments are polymorphic and differ from those in Alzheimer’s and Pick’s diseases
eLife, Feb 5, 2019; DOI: 10.7554/eLife.43584
Immunohistochemistry (IHC) of DRG
Author: Diana M Bautista
Date: May 23, 2020，Views：3173
Original protocol used in research article：
The signaling lipid sphingosine 1-phosphate regulates mechanical pain
eLife, Mar 21, 2018; DOI: 10.7554/eLife.33285
hiPSC maintenance and differentiation
Authors: Swagata Ghatak, Dorit Trudler, Nima Dolatabadi and Stuart A Lipton
Date: May 22, 2020，Views：1291
Original protocol used in research article：
Mechanisms of hyperexcitability in Alzheimer’s disease hiPSC-derived neurons and cerebral organoids vs isogenic controls
eLife, Nov 29, 2019; DOI: 10.7554/eLife.50333
Genus-level phylogenetic tree
Author: Ricardo A. Segovia
Date: May 22, 2020，Views：1047
Original protocol used in research article：
Freezing and water availability structure the evolutionary diversity of trees across the Americas
Science Advances, May 5, 2020; DOI: 10.1126/sciadv.aaz5373
AcrIIA11 homolog discovery and phylogenetic placement
Author: Kevin J Forsberg
Date: May 21, 2020，Views：1147
Original protocol used in research article：
Functional metagenomics-guided discovery of potent Cas9 inhibitors in the human microbiome
eLife, Sep 10, 2019; DOI: 10.7554/eLife.46540
Synthesis of compounds
Author: Samuel T. Jones
Date: May 20, 2020，Views：973
Original protocol used in research article：
Modified cyclodextrins as broad-spectrum antivirals
Science Advances, Jan 28, 2020; DOI: 10.1126/sciadv.aax9318
Golgi preparations
Authors: Alun M Davies and Paulina Carriba
Date: May 20, 2020，Views：1024
Original protocol used in research article：
CD40 is a major regulator of dendrite growth from developing excitatory and inhibitory neurons
eLife, Nov 7, 2017; DOI: 10.7554/eLife.30442
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