Protocols shared by authors via RaP service
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Tau expression and purification
Authors: Wenjuan Zhang, Michel Goedert and Sjors HW Scheres
Date: May 24, 2020，Views：6475
Original protocol used in research article：
Heparin-induced tau filaments are polymorphic and differ from those in Alzheimer’s and Pick’s diseases
eLife, Feb 5, 2019; DOI: 10.7554/eLife.43584
Immunohistochemistry (IHC) of DRG
Author: Diana M Bautista
Date: May 23, 2020，Views：3275
Original protocol used in research article：
The signaling lipid sphingosine 1-phosphate regulates mechanical pain
eLife, Mar 21, 2018; DOI: 10.7554/eLife.33285
hiPSC maintenance and differentiation
Authors: Swagata Ghatak, Dorit Trudler, Nima Dolatabadi and Stuart A Lipton
Date: May 22, 2020，Views：1333
Original protocol used in research article：
Mechanisms of hyperexcitability in Alzheimer’s disease hiPSC-derived neurons and cerebral organoids vs isogenic controls
eLife, Nov 29, 2019; DOI: 10.7554/eLife.50333
Genus-level phylogenetic tree
Author: Ricardo A. Segovia
Date: May 22, 2020，Views：1093
Original protocol used in research article：
Freezing and water availability structure the evolutionary diversity of trees across the Americas
Science Advances, May 5, 2020; DOI: 10.1126/sciadv.aaz5373
AcrIIA11 homolog discovery and phylogenetic placement
Author: Kevin J Forsberg
Date: May 21, 2020，Views：1177
Original protocol used in research article：
Functional metagenomics-guided discovery of potent Cas9 inhibitors in the human microbiome
eLife, Sep 10, 2019; DOI: 10.7554/eLife.46540
Synthesis of compounds
Author: Samuel T. Jones
Date: May 20, 2020，Views：995
Original protocol used in research article：
Modified cyclodextrins as broad-spectrum antivirals
Science Advances, Jan 28, 2020; DOI: 10.1126/sciadv.aax9318
Golgi preparations
Authors: Alun M Davies and Paulina Carriba
Date: May 20, 2020，Views：1060
Original protocol used in research article：
CD40 is a major regulator of dendrite growth from developing excitatory and inhibitory neurons
eLife, Nov 7, 2017; DOI: 10.7554/eLife.30442
Modeling Study design
Author: Sixue Zhang
Date: May 19, 2020，Views：933
Original protocol used in research article：
β-amyloid redirects norepinephrine signaling to activate the pathogenic GSK3β/tau cascade
Science Translational Medicine, Jan 14, 2020; DOI: 10.1126/scitranslmed.aay6931
DNA plasmids and cell lines
Authors: Jason E. Lee and Gia K. Voeltz
Date: May 18, 2020，Views：1004
Original protocol used in research article：
Endoplasmic reticulum contact sites regulate the dynamics of membraneless organelles
Science, Jan 30, 2020; DOI: 10.1126/science.aay7108
Optimization of planarian fixation for RNA extraction and histology
Author: David J Forsthoefel
Date: May 18, 2020，Views：1161
Original protocol used in research article：
Cell-type diversity and regionalized gene expression in the planarian intestine
eLife, Apr 2, 2020; DOI: 10.7554/eLife.52613
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