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In vitro effects of YAP1-targeting drugs
Last updated date: Jul 21, 2020 Views: 914 Forks: 0
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1. Seed HSC-4 cells (1×10^4 cells/well) onto 24-well plates.
HSC-4 cell: JCRB0624
Medium: DMEM/Ham’s F12 medium (1:1) supplemented by 10% FCS.
2. Add chemicals as listed below (or DMSO as vehicle control) to the medium.
3. Culture the cells at 37°C in CO2 incubator for the indicated period.
4. After the incubation for 1, 2, 3 day(s), cell were trypsinized and counted on a conventional hematocytometer.
Chemicals:
Chemical | Company | Catalog Number | Stock solution concentration | Solvent | Final concentraion |
Dasatinib | Abcam | ab142050 | 5 mM | DMSO | 5 μM |
Verteporfin | USP | 1711461 | 5 mM | DMSO | 5 μM |
Simbastatin | TCI | S0509 | 5 mM | DMSO | 5 μM |
- Maehama, T, Suzuki, A and Omori, H(2020). In vitro effects of YAP1-targeting drugs. Bio-protocol Preprint. bio-protocol.org/prep408.
- Omori, H., Nishio, M., Masuda, M., Miyachi, Y., Ueda, F., Nakano, T., Sato, K., Mimori, K., Taguchi, K., Hikasa, H., Nishina, H., Tashiro, H., Kiyono, T., Mak, T. W., Nakao, K., Nakagawa, T., Maehama, T. and Suzuki, A.(2020). YAP1 is a potent driver of the onset and progression of oral squamous cell carcinoma . Science Advances 6(12). DOI: 10.1126/sciadv.aay3324
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