- Home
- Protocols
-
In vitro effects of YAP1-targeting drugs
Last updated date: Jul 21, 2020 Views: 890 Forks: 0
Download PDF
Ask a
question
How to
cite
Favorite
1. Seed HSC-4 cells (1×10^4 cells/well) onto 24-well plates.
HSC-4 cell: JCRB0624
Medium: DMEM/Ham’s F12 medium (1:1) supplemented by 10% FCS.
2. Add chemicals as listed below (or DMSO as vehicle control) to the medium.
3. Culture the cells at 37°C in CO2 incubator for the indicated period.
4. After the incubation for 1, 2, 3 day(s), cell were trypsinized and counted on a conventional hematocytometer.
Chemicals:
Chemical | Company | Catalog Number | Stock solution concentration | Solvent | Final concentraion |
Dasatinib | Abcam | ab142050 | 5 mM | DMSO | 5 μM |
Verteporfin | USP | 1711461 | 5 mM | DMSO | 5 μM |
Simbastatin | TCI | S0509 | 5 mM | DMSO | 5 μM |
- Maehama, T, Suzuki, A and Omori, H(2020). In vitro effects of YAP1-targeting drugs. Bio-protocol Preprint. bio-protocol.org/prep408.
- Omori, H., Nishio, M., Masuda, M., Miyachi, Y., Ueda, F., Nakano, T., Sato, K., Mimori, K., Taguchi, K., Hikasa, H., Nishina, H., Tashiro, H., Kiyono, T., Mak, T. W., Nakao, K., Nakagawa, T., Maehama, T. and Suzuki, A.(2020). YAP1 is a potent driver of the onset and progression of oral squamous cell carcinoma . Science Advances 6(12). DOI: 10.1126/sciadv.aay3324
Category
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Tips for asking effective questions
+ Description
Write a detailed description. Include all information that will help others answer your question including experimental processes, conditions, and relevant images.
Post a Question
