Protocols shared by authors via RaP service
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Model equations
Author: Clara Champagne
Date: May 2, 2020，Views：1131
Original protocol used in research article：
Structure in the variability of the basic reproductive number (R0) for Zika epidemics in the Pacific islands
eLife, Nov 29, 2016; DOI: 10.7554/eLife.19874
Tissue preparation, immunohistochemistry and in situ hybridization
Authors: Jane E Johnson, Juan Villarreal and Trisha Savage
Date: May 1, 2020，Views：2165
Original protocol used in research article：
Repression by PRDM13 is critical for generating precision in neuronal identity
eLife, Aug 29, 2017; DOI: 10.7554/eLife.25787
Creoptix WAVE experiments
Author: Gunther Kern
Date: Apr 30, 2020，Views：1250
Original protocol used in research article：
Dynamics of human protein kinase Aurora A linked to drug selectivity
eLife, Jun 14, 2018; DOI: 10.7554/eLife.36656
Real-time PCR analysis
Author: Natascha Ellen Stumpf
Date: Apr 30, 2020，Views：1039
Original protocol used in research article：
A high-salt diet compromises antibacterial neutrophil responses through hormonal perturbation
Science Translational Medicine, Mar 24, 2020; DOI: 10.1126/scitranslmed.aay3850
Cell fractionation
Authors: Cecilia Julia Ballaré and Luciano Di Croce
Date: Apr 29, 2020，Views：1076
Original protocol used in research article：
PHF19 mediated regulation of proliferation and invasiveness in prostate cancer cells
eLife, Mar 10, 2020; DOI: 10.7554/eLife.51373
ATAC-Seq
Authors: Sarah E Dremel and Neal A DeLuca
Date: Apr 28, 2020，Views：5973
Original protocol used in research article：
Herpes simplex viral nucleoprotein creates a competitive transcriptional environment facilitating robust viral transcription and host shut off
eLife, Oct 22, 2019; DOI: 10.7554/eLife.51109
Concanavalin A enrichment
Authors: Matthew Freeman and Adam G Grieve
Date: Apr 28, 2020，Views：1480
Original protocol used in research article：
FRMD8 promotes inflammatory and growth factor signalling by stabilising the iRhom/ADAM17 sheddase complex
eLife, Jun 13, 2018; DOI: 10.7554/eLife.35012
Fluorescence activated cell sorting in Schistosoma mansoni
Author: George R Wendt
Date: Apr 28, 2020，Views：1130
Original protocol used in research article：
Flatworm-specific transcriptional regulators promote the specification of tegumental progenitors in Schistosoma mansoni
eLife, Mar 20, 2018; DOI: 10.7554/eLife.33221
Bioinformatic analysis of the ChIP-seq and CUT&RUN datasets
Authors: Huacheng Luo and Jianrong Lu
Date: Apr 25, 2020，Views：1530
Original protocol used in research article：
LATS kinase–mediated CTCF phosphorylation and selective loss of genomic binding
Science Advances, Feb 19, 2020; DOI: 10.1126/sciadv.aaw4651
Chromatin immunoprecipitation (ChIP) assay
Authors: Shuchi Agrawal Singh and Klaus Hansen
Date: Apr 24, 2020，Views：2587
Original protocol used in research article：
PLZF targets developmental enhancers for activation during osteogenic differentiation of human mesenchymal stem cells
eLife, Jan 23, 2019; DOI: 10.7554/eLife.40364
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