Protocols shared by authors via RaP service
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FLAG affinity purification and mass spectrometry
Authors: Payal Jain, Cecilia Julia Ballaré and Luciano Di Croce
Date: May 11, 2020，Views：1193
Original protocol used in research article：
PHF19 mediated regulation of proliferation and invasiveness in prostate cancer cells
eLife, Mar 10, 2020; DOI: 10.7554/eLife.51373
Integration site mapping by high-throughput sequencing of Tol2-oligo pulldown-enriched fragments
Authors: Ashwin A Bhandiwad, Tianwei Li, James R Iben, Steven L Coon and Harold A Burgess
Date: May 11, 2020，Views：1363
Original protocol used in research article：
Brain-wide cellular resolution imaging of Cre transgenic zebrafish lines for functional circuit-mapping
eLife, Feb 8, 2019; DOI: 10.7554/eLife.42687
Ligand-receptor networks
Author: Ralph Patrick
Date: May 10, 2020，Views：1241
Original protocol used in research article：
Single-cell expression profiling reveals dynamic flux of cardiac stromal, vascular and immune cells in health and injury
eLife, Mar 26, 2019; DOI: 10.7554/eLife.43882
RT-PCR and quantitative analysis
Author: Emilie Barruet
Date: May 8, 2020，Views：1158
Original protocol used in research article：
Functionally heterogeneous human satellite cells identified by single cell RNA sequencing
eLife, Apr 1, 2020; DOI: 10.7554/eLife.51576
STAG1-AID , STAG2-AID
Author: Wen Tang
Date: May 8, 2020，Views：1161
Original protocol used in research article：
ESCO1 and CTCF enable formation of long chromatin loops by protecting cohesinSTAG1 from WAPL
eLife, Feb 17, 2020; DOI: 10.7554/eLife.52091
Cell lysis
Author: Byoungsan Choi
Date: May 8, 2020，Views：2232
Original protocol used in research article：
Single-molecule functional anatomy of endogenous HER2-HER3 heterodimers
eLife, Apr 8, 2020; DOI: 10.7554/eLife.53934
Sample size calculations and statistical analyses
Author: Asuncion Mejias
Date: May 7, 2020，Views：960
Original protocol used in research article：
Immune profiles provide insights into respiratory syncytial virus disease severity in young children
Science Translational Medicine, Apr 21, 2020; DOI: 10.1126/scitranslmed.aaw0268
Calculating gene correlations with RERconverge
Authors: Maria Chikina and Nathan L Clark
Date: May 7, 2020，Views：1310
Original protocol used in research article：
Subterranean mammals show convergent regression in ocular genes and enhancers, along with adaptation to tunneling
eLife, Oct 16, 2017; DOI: 10.7554/eLife.25884
Aggressive behavior
Author: Chuan Zhou
Date: May 6, 2020，Views：1144
Original protocol used in research article：
A neuropeptide regulates fighting behavior in Drosophila melanogaster
eLife, Apr 21, 2020; DOI: 10.7554/eLife.54229
Isolation and proteomic analysis of mortalin interactome
Authors: Pui-Kei Wu, Rebekah L Gundry and Jong-In Park
Date: May 6, 2020，Views：959
Original protocol used in research article：
Mortalin (HSPA9) facilitates BRAF-mutant tumor cell survival by suppressing ANT3-mediated mitochondrial membrane permeability
Science Signaling, Mar 9, 2020; DOI: 10.1126/scisignal.aay1478
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