Protocols shared by authors via RaP service
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Purification of APP C100-FLAG substrate
Authors: David M Bolduc, Michael S Wolfe and Dennis J Selkoe
Date: Apr 14, 2020，Views：1171
Original protocol used in research article：
The amyloid-beta forming tripeptide cleavage mechanism of γ-secretase
eLife, Aug 31, 2016; DOI: 10.7554/eLife.17578
Step-by-step lamina propria cell isolation protocol for H. polygyrus infected intestines
Author: Johannes U Mayer
Date: Apr 13, 2020，Views：1376
Original protocol used in research article：
High-dimensional analysis of intestinal immune cells during helminth infection
eLife, Feb 11, 2020; DOI: 10.7554/eLife.51678
β-Arrestin recruitment
Authors: Alix A. J. Rouault and Julien A. Sebag
Date: Apr 13, 2020，Views：1109
Original protocol used in research article：
The GPCR accessory protein MRAP2 regulates both biased signaling and constitutive activity of the ghrelin receptor GHSR1a
Science Signaling, Jan 6, 2020; DOI: 10.1126/scisignal.aax4569
Whole mount immunostaining
Author: Manju Sharma
Date: Apr 13, 2020，Views：1233
Original protocol used in research article：
Identification of EOMES-expressing spermatogonial stem cells and their regulation by PLZF
eLife, May 31, 2019; DOI: 10.7554/eLife.43352
Cell surface ELISA
Author: Amanda N Hayward
Date: Apr 13, 2020，Views：1398
Original protocol used in research article：
A toolkit for studying cell surface shedding of diverse transmembrane receptors
eLife, Jun 7, 2019; DOI: 10.7554/eLife.46983
RNP assembly for HDR reaction
Authors: Xinyu Ling and Tao Liu
Date: Apr 13, 2020，Views：1192
Original protocol used in research article：
Improving the efficiency of precise genome editing with site-specific Cas9-oligonucleotide conjugates
Science Advances, Apr 7, 2020; DOI: 10.1126/sciadv.aaz0051
Expression and purification of azido-Cas9 mutants
Authors: Xinyu Ling and Tao Liu
Date: Apr 13, 2020，Views：1238
Original protocol used in research article：
Improving the efficiency of precise genome editing with site-specific Cas9-oligonucleotide conjugates
Science Advances, Apr 7, 2020; DOI: 10.1126/sciadv.aaz0051
Bacterial culturing
Authors: Lina Yao and A Sloan Devlin
Date: Apr 11, 2020，Views：1127
Original protocol used in research article：
A selective gut bacterial bile salt hydrolase alters host metabolism
eLife, Jul 17, 2018; DOI: 10.7554/eLife.37182
Docking, pharmacophore modeling, virtual screening, and selection of test compounds
Authors: Teresa Kaserer and Daniela Schuster
Date: Apr 11, 2020，Views：1672
Original protocol used in research article：
Modulating FOXO3 transcriptional activity by small, DBD-binding molecules
eLife, Dec 4, 2019; DOI: 10.7554/eLife.48876
Identification of methylation markers discriminating between CRC and normal blood
Authors: Huiyan Luo and Rui-hua Xu
Date: Apr 10, 2020，Views：961
Original protocol used in research article：
Circulating tumor DNA methylation profiles enable early diagnosis prognosis prediction and screening for colorectal cancer
Science Translational Medicine, Dec 31, 2019; DOI: 10.1126/scitranslmed.aax7533
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