O2 release from PCC7942

1.    The S. elongatus PCC7942 were incubated in BG-11 liquid medium in 150-ml conical flasks under 12 h light/dark conditions.

2.    PCC7942 was collected by centrifugation at 8000 rpm for 5 min during it’s logarithmic phase.

3.    The supernatants were removed and then the precipitates were repeatedly washed three times with phosphate-buffered saline (PBS).

4.    The precipitates were redispersed in PBS with 500 μM Na₂CO₃.

5.    PCC7942 (1 × 10⁹ cells/ml) solution was added into a penicillin bottle and exposed to a 620- to 660-nm near-infrared light-emitting         diode (NIR LED) at 0.5 W/cm2 power density.

6.    The O₂ concentration of PCC7942 were measured by the oxygen electrodes (OX-N-904151, Unisense).

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