This protocol was submitted by the author(s) of the original paper via Bio-protocol's "Request a Protocol" feature.
Original Research Paper STK25 suppresses Hippo signaling by regulating SAV1-STRIPAK antagonism
DOI: 10.7554/eLife.54863
eLIFE , Apr 15, 2020
Related protocols in the same article
Dephosphorylation assay
Sung Jun Bae    Xuelian Luo    
Original Research Paper
Last updated date: Jul 11, 2020 View: 972


Recipes

Materials:

1.     Lysis Buffer : 20 mM Tris-HCl (pH 7.5), 150 mM NaCl and 0.2% Triton X-100. (Do not add EDTA since it inactivates CIP.)

2.     EDTA-free Protease Inhibitor Cocktail : #11873580001, Roche

3.     CIP Buffer: 50 mM Tris-HCl (pH 7.9), 100 mM NaCl, 10 mM MgCl2, and 1 mM Dithiothreitol (DTT)

4.     CIP (calf intestinal phosphatase): M0290, New England Biolabs

References

Reference:

Robison, J.G., Elliott, J., Dixon, K., and Oakley, G.G. 2004. Replication protein A and the Mre11.Rad50.Nbs1 complex co-localize and interact at sites of stalled replication forks. The Journal of biological chemistry 279: 34802-34810.


Related files
How to cite: Readers should cite both the Bio-protocol article and the original research article where this protocol was used:
1. Bae, S. and Luo, X. (2020). Dephosphorylation assay. Bio-protocol. bio-protocol.org/prep381.
2. Bae, S., Ni, L. and Luo, X.(2020). STK25 suppresses Hippo signaling by regulating SAV1-STRIPAK antagonism. eLIFE . DOI: 10.7554/eLife.54863
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