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Real-time quantitative PCR

SS Sunhye Shin

Last updated date: Jul 27, 2020 Views: 1135 Forks: 0

original An abbreviated version of this protocol was published in eLife in Jun, 2020
Dynamic control of adipose tissue development and adult tissue homeostasis by platelet-derived growth factor receptor alpha
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To analyze the qPCR data, we used the 2^(-ΔΔCt) method. 

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How to cite：
Readers should cite both the Bio-protocol preprint and the original research article where this protocol was used:
  1. Shin, S(2020). Real-time quantitative PCR. Bio-protocol Preprint. bio-protocol.org/prep415.
  2. Shin, S., Pang, Y., Park, J., Liu, L., Lukas, B. E., Kim, S. H., Kim, K., Xu, P., Berry, D. C. and Jiang, Y.(2020). Dynamic control of adipose tissue development and adult tissue homeostasis by platelet-derived growth factor receptor alpha. eLife. DOI: 10.7554/eLife.56189

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