细胞生物学

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    现刊
    Quantitative Live Confocal Imaging in Aquilegia Floral Meristems
    白葵花分生组织的定量实时共聚焦成像
    作者:Ya Min, Stephanie J. Conway and Elena M. Kramer日期:06/20/2022,浏览量:980,Q&A: 0

    In this study, we present a detailed protocol for live imaging and quantitative analysis of floral meristem development in Aquilegia coerulea, a member of the buttercup family (Ranunculaceae). Using confocal microscopy and the image analysis

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    Fluorescent Labeling of Small Extracellular Vesicles (EVs) Isolated from Conditioned Media
    从条件培养基中分离的细胞外小泡的荧光标记
    作者:John Santelices, Mark Ou, Winnie W. Hui, Gustavo H. B. Maegawa and Mariola J. Edelmann日期:06/20/2022,浏览量:1536,Q&A: 0

    Extracellular vesicles (EVs), such as exosomes, are produced by all known eukaryotic cells, and constitute essential means of intercellular communication. Recent studies have unraveled the important roles of EVs in migrating to specific sites and

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    Quantitative Live Confocal Imaging in Aquilegia Floral Meristems
    白葵花分生组织的定量实时共聚焦成像
    作者:Ya Min, Stephanie J. Conway and Elena M. Kramer日期:06/20/2022,浏览量:980,Q&A: 0
    [Abstract]

    In this study, we present a detailed protocol for live imaging and quantitative analysis of floral meristem development in Aquilegia coerulea, a member of the buttercup family (Ranunculaceae). Using confocal microscopy and the image analysis software MorphoGraphX, we were able to examine the cellular growth dynamics during floral organ primordia

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    Fluorescent Labeling of Small Extracellular Vesicles (EVs) Isolated from Conditioned Media
    从条件培养基中分离的细胞外小泡的荧光标记
    作者:John Santelices, Mark Ou, Winnie W. Hui, Gustavo H. B. Maegawa and Mariola J. Edelmann日期:06/20/2022,浏览量:1536,Q&A: 0
    [Abstract]

    Extracellular vesicles (EVs), such as exosomes, are produced by all known eukaryotic cells, and constitute essential means of intercellular communication. Recent studies have unraveled the important roles of EVs in migrating to specific sites and cells. Functional studies of EVs using in vivo and in vitro systems require tracking these organelles

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    Labelling of Active Transcription Sites with Argonaute NRDE-3—Image Active Transcription Sites in vivo in Caenorhabditis elegans
    用Argonaute NRDE-3标记活性转录位点—图像秀丽隐杆线虫体内的活性转录位点
    作者:Antoine Barrière and Vincent Bertrand日期:06/05/2022,浏览量:509,Q&A: 0
    [Abstract]

    Live labelling of active transcription sites is critical to our understanding of transcriptional dynamics. In the most widely used method, RNA sequence MS2 repeats are added to the transcript of interest, on which fluorescently tagged Major Coat Protein binds, and labels transcription sites and transcripts. Here we describe another strategy, using

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    Electroporation of Small Interfering RNAs into Tibialis Anterior Muscles of Mice
    将小干扰 RNA 电穿孔到小鼠胫骨前肌中
    作者:Anna Stephan, Flavia A. Graca, Liam C. Hunt and Fabio Demontis日期:06/05/2022,浏览量:780,Q&A: 0
    [Abstract]

    Aging and wasting of skeletal muscle reduce organismal fitness. Regrettably, only limited interventions are currently available to address this unmet medical need. Many methods have been developed to study this condition, including the intramuscular electroporation of DNA plasmids. However, this technique requires surgery and high electrical

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    Plasma Membrane Wounding and Repair Assays for Eukaryotic Cells
    真核细胞的等离子膜损伤和修复试验
    [Abstract]

    Damage to the plasma membrane and loss of membrane integrity are detrimental to eukaryotic cells. It is, therefore, essential that cells possess an efficient membrane repair system to survive. However, the different cellular and molecular mechanisms behind plasma membrane repair have not been fully elucidated. Here, we present three complementary

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    Assessing the Presence of Hematopoietic Stem and Progenitor Cells in Mouse Spleen
    评估小鼠脾脏中造血干细胞和祖细胞的存在
    作者:Isabelle J. Marié, Lara Brambilla and David E. Levy日期:06/05/2022,浏览量:644,Q&A: 0
    [Abstract]

    Transplantation of hematopoietic material into recipient mice is an assay routinely used to determine the presence and function of hematopoietic stem and progenitor cells (HSPCs) in vivo. The principle of the method is to transplant donor cells being tested for HSPCs into a recipient mouse following bone marrow ablation and testing for

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    Simple Methods for Permanent or Transient Denervation in Mouse Sciatic Nerve Injury Models
    小鼠坐骨神经损伤模型中永久性或暂时性去神经的简单方法
    作者:Alexis Osseni, Jean-Luc Thomas, Alireza Ghasemizadeh, Laurent Schaeffer and Vincent Gache日期:06/05/2022,浏览量:594,Q&A: 0
    [Abstract]

    Our ability to move and breathe requires an efficient communication between nerve and muscle that mainly takes place at the neuromuscular junctions (NMJs), a highly specialized synapse that links the axon of a motor neuron to a muscle fiber. When NMJs or axons are disrupted, the control of muscle fiber contraction is lost and muscle are paralyzed.

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    Flow Cytometric Characterization of Macrophages Infected in vitro with Salmonella enterica Serovar Typhimurium Expressing Red Fluorescent Protein
    表达红色荧光蛋白的肠沙门氏菌血清鼠伤寒菌体外感染巨噬细胞的流式细胞术研究
    作者:Natascha Brigo, Christa Pfeifhofer-Obermair, Egon Demetz, Piotr Tymoszuk and Günter Weiss日期:06/05/2022,浏览量:431,Q&A: 0
    [Abstract]

    Macrophages are important for host defense against intracellular pathogens like Salmonella and can be differentiated into two major subtypes. M1 macrophages, which are pro-inflammatory and induce antimicrobial immune effector mechanisms, including the expression of inducible nitric oxide synthase (iNOS), and M2 macrophages, which exert

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    Efficient Superovulation and Egg Collection from Mice
    高效的超排卵和小鼠卵子收集
    作者:Miyuki Shindo, Kenji Miyado, Woojin Kang, Maki Fukami and Mami Miyado日期:06/05/2022,浏览量:547,Q&A: 0
    [Abstract]

    Superovulation is a method used to reduce the number of mice used per experiment by increasing the egg number. Conventionally, superovulation for obtaining mouse eggs involves the use of equine chorionic gonadotropin (eCG) for stimulation and human CG for induction. Female mice of the C57BL/6 inbred strain spontaneously ovulate approximately 10

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    Optimized CRISPR-Cas9-based Strategy for Complex Gene Targeting in Murine Embryonic Stem Cells for Germline Transmission
    用于生殖系传递的基于 CRISPR-Cas9 的小鼠胚胎干细胞中优化的复杂基因靶向策略
    作者:Thomas J. O'Neill, Daniel Krappmann and Andreas Gewies日期:05/20/2022,浏览量:811,Q&A: 0
    [Abstract]

    Although CRISPR-Cas9 genome editing can be performed directly in single-cell mouse zygotes, the targeting efficiency for more complex modifications such as the insertion of two loxP sites, multiple mutations in cis, or the precise insertion or deletion of longer DNA sequences often remains low (Cohen, 2016). Thus, targeting and validation of

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