生物物理学

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    现刊
    Patterned Substrate of Mobile and Immobile Ligands to Probe EphA2 Receptor Clustering
    用于探测 EphA2 受体聚类的移动和固定配体的图案化底物
    作者:Zhongwen Chen, Kabir H. Biswas and Jay T. Groves日期:06/05/2022,浏览量:769,Q&A: 0
    [Abstract]

    A multitude of membrane-localized receptors are utilized by cells to integrate both biochemical and physical signals from their microenvironment. The clustering of membrane receptors is widely presumed to have functional consequences for subsequent signal transduction. However, it is experimentally challenging to selectively manipulate receptor

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    Purification of Mitochondrial Ribosomal Complexes from Trypanosoma cruzi and Leishmania tarentolae for Cryo-EM Analysis
    克氏锥虫和塔氏利什曼原虫线粒体核糖体复合物的纯化及低温电子显微镜分析
    作者:Stéphanie Durrieu-Gaillard, Marie Sissler and Yaser Hashem日期:05/20/2022,浏览量:905,Q&A: 1
    [Abstract]

    Kinetoplastids are unicellular eukaryotic parasites responsible for human pathologies such as Chagas disease, sleeping sickness or Leishmaniasis, caused by Trypanosoma cruzi, Trypanosoma brucei, and various Leishmania spp., respectively. They harbor a single large mitochondrion that is essential for the survival of the parasite. Interestingly,

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    Production and Crystallization of Nanobodies in Complex with the Receptor Binding Domain of the SARS-CoV-2 Spike Protein
    与 SARS-CoV-2 刺突蛋白受体结合域复合的纳米抗体的产生和结晶
    [Abstract]

    The receptor binding domain (RBD) of the spike protein of SARS-CoV-2 binds angiotensin converting enzyme-2 (ACE-2) on the surface of epithelial cells, leading to fusion, and entry of the virus into the cell. This interaction can be blocked by the binding of llama-derived nanobodies (VHHs) to the RBD, leading to virus neutralisation. Structural

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    Quantitative Analysis of Actin Cable Length in Yeast
    酵母中肌动蛋白索长度的定量分析
    作者:Shane G. McInally, Jane Kondev and Bruce L. Goode日期:05/05/2022,浏览量:927,Q&A: 0
    [Abstract]

    Polarized actin cables in S. cerevisiae are linear bundles of crosslinked actin filaments that are assembled by two formins, Bnr1 (localized to the bud neck), and Bni1 (localized to the bud tip). Actin is polymerized at these two sites, which results in cables extending along the cell cortex toward the back of the mother cell. These cables serve

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    Characterization of the Elasticity of CD4+ T Cells: An Approach Based on Peak Force Quantitative Nanomechanical Mapping
    CD4+T细胞弹性的特征:一个基于峰值力定量纳米力学图的方法
    作者:Philipp Jung, Xiangda Zhou, Sandra Iden, Bin Qu and Markus Bischoff日期:04/20/2022,浏览量:899,Q&A: 0
    [Abstract]

    CD4+ T cells are essential players in orchestrating the specific immune response against intracellular pathogens, and in inhibiting tumor development in an early stage. The activation of T cells is triggered by engagement of T cell receptors (TCRs). Here, CD3 and CD28 molecules are key factors, (co)stimulating signaling pathways essential for

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    Bsoft: Image Processing for Structural Biology
    Bsoft:结构生物学的图像处理
    作者:J. Bernard Heymann日期:04/20/2022,浏览量:1186,Q&A: 0
    [Abstract]

    Bsoft is a software package primarily developed for processing electron micrographs, with the goal of determining the structures of biologically relevant molecules, molecular assemblies, and parts of cells. However, it incorporates many ways to deal with images, from the mundane to very sophisticated algorithms. This article is an introduction

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    Single Molecule Tracking Nanoscopy Extended to Two Colors with MTT2col for the Analysis of Cell-Cell Interactions in Leukemia
    使用 MTT2col 将单分子跟踪纳米显微镜扩展到两种颜色,用于分析白血病中的细胞-细胞相互作用
    作者:Loriane Maillot, Magali Irla and Arnauld Sergé日期:04/20/2022,浏览量:802,Q&A: 0
    [Abstract]

    Single molecule tracking (SMT) is a powerful technique to study molecular dynamics, and is particularly adapted to monitor the motion and interactions of cell membrane components. Assessing interactions among two molecular populations is classically performed by several approaches, including dual-color videomicroscopy, which allows monitoring of

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    Activation of Mitochondrial Ca2+ Oscillation and Mitophagy Induction by Femtosecond Laser Photostimulation
    飞秒激光光刺激激活线粒体 Ca2+ 振荡和线粒体自噬诱导
    作者:Xiaoying Tian and Hao He日期:04/05/2022,浏览量:435,Q&A: 0
    [Abstract]

    Ultra-precise stimulation solely to individual mitochondria, without any influence to the whole cell, is quite difficult by traditional biochemical reagents. In mitophagy research, the mitochondria and even the whole cell usually suffer irreversible and great damage caused by treatment with potent chemicals. In this protocol, we present the

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    Quantitative and Anatomical Imaging of Human Skin by Noninvasive Photoacoustic Dermoscopy
    无创光声皮肤镜对人体皮肤的定量和解剖成像
    作者:Zhiyang Wang, Fei Yang, Wuyu Zhang and Sihua Yang日期:04/05/2022,浏览量:501,Q&A: 0
    [Abstract]

    Imaging plays a vital role in the diagnosis and treatment of skin diseases. However, pure optical imaging technique is limited to the visualization of superficial skin tissues. Ultrasonic imaging technique can detect deep tissues, but it lacks detailed information on microscopic pathological structures. Photoacoustic imaging is an advanced

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    Electrophysiological Measurements of Isolated Blood Vessels
    离体血管的电生理测量
    作者:Samuel A Molina, Daniela Maier-Begandt, Brant E Isakson and Michael Koval日期:03/20/2022,浏览量:584,Q&A: 0
    [Abstract]

    The lumen of blood vessels is covered by endothelial cells, which regulate their permeability to ions and solutes. Endothelial permeability depends on the vascular bed and cell phenotype, and is influenced by different disease states. Most characterization of endothelial permeability has been carried out using isolated cells in culture. While

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