发育生物学

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    现刊
    ATAC-Seq of a Single Myofiber from Mus musculus
    小家鼠单一肌纤维的ATAC-Seq
    作者:Korin Sahinyan, Darren M. Blackburn and Vahab D. Soleimani日期:06/20/2022,浏览量:1181,Q&A: 0

    Chromatin accessibility is a key determinant of gene expression that can be altered under different physiological and disease conditions. Skeletal muscle is made up of myofibers that are highly plastic and adaptive. Therefore, assessing the

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    Quantitative Live Confocal Imaging in Aquilegia Floral Meristems
    白葵花分生组织的定量实时共聚焦成像
    作者:Ya Min, Stephanie J. Conway and Elena M. Kramer日期:06/20/2022,浏览量:980,Q&A: 0

    In this study, we present a detailed protocol for live imaging and quantitative analysis of floral meristem development in Aquilegia coerulea, a member of the buttercup family (Ranunculaceae). Using confocal microscopy and the image analysis

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    ATAC-Seq of a Single Myofiber from Mus musculus
    小家鼠单一肌纤维的ATAC-Seq
    作者:Korin Sahinyan, Darren M. Blackburn and Vahab D. Soleimani日期:06/20/2022,浏览量:1181,Q&A: 0
    [Abstract]

    Chromatin accessibility is a key determinant of gene expression that can be altered under different physiological and disease conditions. Skeletal muscle is made up of myofibers that are highly plastic and adaptive. Therefore, assessing the genome-wide chromatin state of myofibers under various conditions is very important to gain insight into the

    ...
    Quantitative Live Confocal Imaging in Aquilegia Floral Meristems
    白葵花分生组织的定量实时共聚焦成像
    作者:Ya Min, Stephanie J. Conway and Elena M. Kramer日期:06/20/2022,浏览量:980,Q&A: 0
    [Abstract]

    In this study, we present a detailed protocol for live imaging and quantitative analysis of floral meristem development in Aquilegia coerulea, a member of the buttercup family (Ranunculaceae). Using confocal microscopy and the image analysis software MorphoGraphX, we were able to examine the cellular growth dynamics during floral organ primordia

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    Labelling of Active Transcription Sites with Argonaute NRDE-3—Image Active Transcription Sites in vivo in Caenorhabditis elegans
    用Argonaute NRDE-3标记活性转录位点—图像秀丽隐杆线虫体内的活性转录位点
    作者:Antoine Barrière and Vincent Bertrand日期:06/05/2022,浏览量:509,Q&A: 0
    [Abstract]

    Live labelling of active transcription sites is critical to our understanding of transcriptional dynamics. In the most widely used method, RNA sequence MS2 repeats are added to the transcript of interest, on which fluorescently tagged Major Coat Protein binds, and labels transcription sites and transcripts. Here we describe another strategy, using

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    Electroporation of Small Interfering RNAs into Tibialis Anterior Muscles of Mice
    将小干扰 RNA 电穿孔到小鼠胫骨前肌中
    作者:Anna Stephan, Flavia A. Graca, Liam C. Hunt and Fabio Demontis日期:06/05/2022,浏览量:780,Q&A: 0
    [Abstract]

    Aging and wasting of skeletal muscle reduce organismal fitness. Regrettably, only limited interventions are currently available to address this unmet medical need. Many methods have been developed to study this condition, including the intramuscular electroporation of DNA plasmids. However, this technique requires surgery and high electrical

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    Simple Methods for Permanent or Transient Denervation in Mouse Sciatic Nerve Injury Models
    小鼠坐骨神经损伤模型中永久性或暂时性去神经的简单方法
    作者:Alexis Osseni, Jean-Luc Thomas, Alireza Ghasemizadeh, Laurent Schaeffer and Vincent Gache日期:06/05/2022,浏览量:594,Q&A: 0
    [Abstract]

    Our ability to move and breathe requires an efficient communication between nerve and muscle that mainly takes place at the neuromuscular junctions (NMJs), a highly specialized synapse that links the axon of a motor neuron to a muscle fiber. When NMJs or axons are disrupted, the control of muscle fiber contraction is lost and muscle are paralyzed.

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    Click-iT® Plus OPP Alexa Fluor® Protein Synthesis Assay in Embryonic Cells
    在胚胎细胞中Click- iT®Plus OPP Alexa Fluor®蛋白合成分析
    作者:Yan Li, Xu Ji, Lu Chang, Jianan Tang, Min-Min Hua, Jing Liu, Christopher O’Neil, Xuefeng Huang and Xingliang Jin日期:06/05/2022,浏览量:600,Q&A: 0
    [Abstract]

    This protocol describes a method to assess relative changes in the level of global protein synthesis in the preimplantation embryo using the Click-iT® Plus OPP Protein Synthesis Assays. In this assay, O-propargyl-puromycin (OPP), an analog of puromycin, is efficiently incorporated into the nascent polypeptide of newly translated proteins in

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    Efficient Superovulation and Egg Collection from Mice
    高效的超排卵和小鼠卵子收集
    作者:Miyuki Shindo, Kenji Miyado, Woojin Kang, Maki Fukami and Mami Miyado日期:06/05/2022,浏览量:547,Q&A: 0
    [Abstract]

    Superovulation is a method used to reduce the number of mice used per experiment by increasing the egg number. Conventionally, superovulation for obtaining mouse eggs involves the use of equine chorionic gonadotropin (eCG) for stimulation and human CG for induction. Female mice of the C57BL/6 inbred strain spontaneously ovulate approximately 10

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    Preparation of a Single Cell Suspension from the Murine Iridocorneal Angle
    从小鼠虹膜角膜角制备单细胞悬液
    作者:Benjamin R. Thomson and Susan E. Quaggin日期:05/20/2022,浏览量:578,Q&A: 0
    [Abstract]

    Single cell RNA sequencing is a powerful tool that can be used to identify distinct cell types and transcriptomic differences within complex tissues. It has proven to be especially useful in tissues of the eye, where investigators have identified novel cell types within the retina, anterior chamber, and iridocorneal angle and explored

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    Isolation of tdTomato Expressing Inter-follicular Epidermal Melanocytes or Keratinocytes from Mouse Tail Skin
    从小鼠尾部皮肤中分离tdTomato表达滤泡间表皮黑色素细胞或角质形成细胞
    作者:Samuel Pop, Oscar Urtatiz and Catherine D. Van Raamsdonk日期:04/20/2022,浏览量:1106,Q&A: 0
    [Abstract]

    The epidermis is the outermost layer of the skin. It is made up of mostly keratinocytes along with a small number of melanocytes and Langerhans cells. Melanocytes produce a pigment called melanin, which is transferred to the keratinocytes, and protects these cells from damage from UV radiation, as well as generating hair and skin colours. In this

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    Visualization and Purification of Caenorhabditis elegans Germ Granule Proteins Using Proximity Labeling
    利用近似标记可视化和纯化秀丽隐杆线虫的胚芽颗粒蛋白
    作者:Hannah L. Hertz, Ian F. Price and Wen Tang日期:04/20/2022,浏览量:1191,Q&A: 0
    [Abstract]

    Membraneless organelles, such as germ granules and stress granules, are liquid-like condensates formed by phase transition. Recently, we and others have adopted proximity-based labeling methods to determine the composition of these membraneless compartments. Here, we describe the use of TurboID—an engineered promiscuous biotin

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