微生物学

Advertisement

分类

    现刊
    A Modified Fluctuation Assay with a CAN1 Reporter in Yeast
    酵母中CAN1报告基因的改良波动测定
    作者:Pengyao Jiang, Anja R. Ollodart and Maitreya J. Dunham日期:06/05/2022,浏览量:518,Q&A: 0
    [Abstract]

    Understanding the generation of mutations is fundamental to understanding evolution and genetic disease; however, the rarity of such events makes experimentally identifying them difficult. Mutation accumulation (MA) methods have been widely used. MA lines require serial bottlenecks to fix de novo mutations, followed by whole-genome sequencing.

    ...
    Flow Cytometry-based Measurement of Reactive Oxygen Species in Cyanobacteria
    基于流式细胞仪的蓝藻活性氧测量
    作者:Soumila Mondal and Shailendra P. Singh日期:05/20/2022,浏览量:629,Q&A: 0
    [Abstract]

    Cyanobacteria are Gram-negative oxygen-producing photosynthetic bacteria that are useful in the pharmaceutical and biofuel industries. Monitoring of oxidative stress under fluctuating environmental conditions is important for determining the fitness, survival, and growth of cyanobacteria in the laboratory as well as in large scale cultivation

    ...
    Purification of the Bacterial Amyloid “Curli” from Salmonella enterica Serovar Typhimurium and Detection of Curli from Infected Host Tissues
    从沙门氏菌鼠伤寒血清中纯化细菌淀粉样蛋白“Curli”并检测受感染宿主组织中的 Curli
    [Abstract]

    Microbiologists are learning to appreciate the importance of “functional amyloids” that are produced by numerous bacterial species and have impacts beyond the microbial world. These structures are used by bacteria to link together, presumably to increase survival, protect against harsh conditions, and perhaps to influence cell-cell

    ...
    A Molecular Cloning and Sanger Sequencing-based Protocol for Detecting Site-specific DNA Methylation
    一种基于分子克隆和Sanger测序的位点特异性DNA甲基化检测方案
    作者:Wei Guo, Anthony Cannon and Damon Lisch日期:05/05/2022,浏览量:680,Q&A: 1
    [Abstract]

    DNA methylation is a conserved chemical modification, by which methyl groups are added to the cytosine of DNA molecules. Methylation can influence gene expression without changing the sequence of a particular gene. This epigenetic effect is an intriguing phenomenon that has puzzled biologists for years. By probing the temporal and spatial patterns

    ...
    Expression, Purification, and in vitro Enzyme Activity Assay of a Recombinant Aldehyde Dehydrogenase from Thermus thermophilus, using an Escherichia coli host
    使用大肠杆菌宿主的嗜热栖热菌重组醛脱氢酶的表达、纯化和体外酶活性测定
    作者:Kim Shortall, Edmond Magner and Tewfik Soulimane日期:05/05/2022,浏览量:743,Q&A: 0
    [Abstract]

    Based on previous in-depth characterisation, aldehyde dehydrogenases (ALDH) are a diverse superfamily of enzymes, in terms of both structure and function, present in all kingdoms of life. They catalyse the oxidation of an aldehyde to carboxylic acid using the cofactor nicotinamide adenine dinucleotide (phosphate) (NAD(P)+), and are often not

    ...
    Ex vivo Human Skin Infection with Herpes Simplex Virus 1
    人皮肤单纯疱疹病毒1的体外感染
    作者:Nydia C. De La Cruz, Maureen Möckel, Lisa Wirtz and Dagmar Knebel-Mörsdorf日期:05/05/2022,浏览量:473,Q&A: 0
    [Abstract]

    Although herpes simplex virus 1 (HSV-1) is a well-studied virus, how the virus invades its human host via skin and mucosa to reach its receptors and initiate infection remains an open question. For studies of HSV-1 infection in skin, mice have been used as animal models. Murine skin infection can be induced after injection or scratching of the

    ...
    Bacterial Growth Curve Measurements with a Multimode Microplate Reader
    使用多模式酶标仪测量细菌生长曲线
    作者:Ariel T. Rogers, Kaitlin R. Bullard, Akash C. Dod and Yong Wang日期:05/05/2022,浏览量:996,Q&A: 0
    [Abstract]

    Bacterial studies based on growth curves are common in microbiology and related fields. Compared to the standard photometer and cuvette based protocols, bacterial growth curve measurements with microplate readers provide better temporal resolution, higher efficiency, and are less laborious, while analysis and interpretation of the microplate-based

    ...
    Production of Recombinant Hepatitis B virus (HBV) and Detection of HBV in Infected Human Liver Organoids
    重组乙型肝炎病毒 (HBV) 的产生和受感染的人类肝脏类器官中 HBV 的检测
    作者:Tanvir Hossain, Shahla Romal and Tokameh Mahmoudi日期:04/20/2022,浏览量:539,Q&A: 0
    [Abstract]

    The absence of long term, primary untransformed in vitro models that support hepatitis B virus (HBV) infection and replication have hampered HBV pre-clinical research, which was reflected in the absence of a curative therapy until recently. One of the limitations for in vitro HBV research has been the absence of high titer and pure recombinant HBV

    ...
    Tracking the Reversed Oxidative Tricarboxylic Acid Cycle in Bacteria
    追踪细菌中的反向氧化三羧酸循环
    作者:Lydia Steffens, Eugenio Pettinato, Thomas M. Steiner, Wolfgang Eisenreich and Ivan A. Berg日期:03/20/2022,浏览量:977,Q&A: 0
    [Abstract]

    Different pathways for autotrophic CO2 fixation can be recognized by the presence of genes for their specific key enzymes. On this basis, (meta)genomic, (meta)transcriptomic, or (meta)proteomic analysis enables the identification of the role of an organism or a distinct pathway in primary production. However, the recently discovered variant of the

    ...
    Phytophthora sojae Transformation Based on the CRISPR/Cas9 System
    基于CRISPR/Cas9系统的大豆疫霉转化
    作者:Jingting Cao, Min Qiu, Wenwu Ye and Yuanchao Wang日期:03/20/2022,浏览量:752,Q&A: 0
    [Abstract]

    Phytophthora sojae is a model species for the study of plant pathogenic oomycetes. The initial research on gene function using Phytophthora was mainly based on gene silencing technology. Recently, the CRISPR/Cas9-mediated genome editing technology was successfully established in P. sojae and widely used in oomycetes. In this protocol, we describe

    ...