植物科学

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    现刊
    Quantitative Live Confocal Imaging in Aquilegia Floral Meristems
    白葵花分生组织的定量实时共聚焦成像
    作者:Ya Min, Stephanie J. Conway and Elena M. Kramer日期:06/20/2022,浏览量:980,Q&A: 0

    In this study, we present a detailed protocol for live imaging and quantitative analysis of floral meristem development in Aquilegia coerulea, a member of the buttercup family (Ranunculaceae). Using confocal microscopy and the image analysis

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    Quantitative Analysis of Redox Pool (NAD+, NADH Content) in Plant Samples Under Aluminum Stress
    铝胁迫下植物样品氧化还原池(NAD+, NADH含量)的定量分析
    作者:Jay Prakash Awasthi, Bedabrata Saha, Hiroyuki Koyama and Sanjib Kumar Panda日期:06/20/2022,浏览量:954,Q&A: 0

    Nicotinamide adenine dinucleotide (NAD) is an essential cofactor of numerous enzymatic reactions found in all living cells. Pyridine nucleotides (NAD+ and NADH) are also key players in signaling through reactive oxygen species (ROS), being crucial

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    Quantitative Live Confocal Imaging in Aquilegia Floral Meristems
    白葵花分生组织的定量实时共聚焦成像
    作者:Ya Min, Stephanie J. Conway and Elena M. Kramer日期:06/20/2022,浏览量:980,Q&A: 0
    [Abstract]

    In this study, we present a detailed protocol for live imaging and quantitative analysis of floral meristem development in Aquilegia coerulea, a member of the buttercup family (Ranunculaceae). Using confocal microscopy and the image analysis software MorphoGraphX, we were able to examine the cellular growth dynamics during floral organ primordia

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    Quantitative Analysis of Redox Pool (NAD+, NADH Content) in Plant Samples Under Aluminum Stress
    铝胁迫下植物样品氧化还原池(NAD+, NADH含量)的定量分析
    作者:Jay Prakash Awasthi, Bedabrata Saha, Hiroyuki Koyama and Sanjib Kumar Panda日期:06/20/2022,浏览量:954,Q&A: 0
    [Abstract]

    Nicotinamide adenine dinucleotide (NAD) is an essential cofactor of numerous enzymatic reactions found in all living cells. Pyridine nucleotides (NAD+ and NADH) are also key players in signaling through reactive oxygen species (ROS), being crucial in the regulation of both ROS-producing and ROS-consuming systems in plants. NAD content is a

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    Investigation of Transposon DNA Methylation and Copy Number Variation in Plants Using Southern Hybridisation
    利用Southern杂交技术研究植物中转座子DNA甲基化和拷贝数变异的情况
    作者:Vivek Hari Sundar G. and P. V. Shivaprasad日期:06/05/2022,浏览量:497,Q&A: 0
    [Abstract]

    Plant genomes are pronouncedly enriched in repeat elements such as transposons. These repeats are epigenetically regulated by DNA methylation. Whole genome high-depth sequencing after bisulfite treatment remains an expensive and laborious method to reliably profile the DNA methylome, especially when considering large genomes such as in crops.

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    In vitro Assays to Evaluate Specificity and Affinity in Protein-phospholipid Interactions
    评估蛋白质-磷脂相互作用的特异性和亲和力的体外测定
    [Abstract]

    Protein–lipid interactions play important roles in many biological processes, including metabolism, signaling, and transport; however, computational and structural analyses often fail to predict such interactions, and determining which lipids participate in these interactions remains challenging. In vitro assays to assess the physical

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    Flow Cytometry-based Measurement of Reactive Oxygen Species in Cyanobacteria
    基于流式细胞仪的蓝藻活性氧测量
    作者:Soumila Mondal and Shailendra P. Singh日期:05/20/2022,浏览量:630,Q&A: 0
    [Abstract]

    Cyanobacteria are Gram-negative oxygen-producing photosynthetic bacteria that are useful in the pharmaceutical and biofuel industries. Monitoring of oxidative stress under fluctuating environmental conditions is important for determining the fitness, survival, and growth of cyanobacteria in the laboratory as well as in large scale cultivation

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    Quantification of Soil-surface Roots in Seedlings and Mature Rice Plants
    幼苗和成熟水稻植物土壤表面根的量化
    作者:Eiko Hanzawa, Yuka Kitomi, Yusaku Uga and Tadashi Sato日期:05/05/2022,浏览量:1057,Q&A: 0
    [Abstract]

    Soil-surface roots (SORs) in rice are primary roots that elongate over or near the soil surface. SORs help avoid excessive reduction of stress that occurs in paddy, such as in saline conditions. SORs may also be beneficial for rice growth in phosphorus-deficient paddy fields. Thus, SOR is a useful trait for crop adaptation to certain environmental

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    Apoplastic Expression of CARD1-ecto Domain in Nicotiana benthamiana and Purification from the Apoplastic Fluids
    本氏烟草中 CARD1-ecto 结构域的质外体表达及质外体液的纯化
    作者:Nobuaki Ishihama, Anuphon Laohavisit, Kaori Takizawa and Ken Shirasu日期:04/20/2022,浏览量:974,Q&A: 0
    [Abstract]

    The protein expression and purification process is an essential initial step for biochemical analysis of a protein of interest. Traditionally, heterologous protein expression systems (such as E. coli, yeast, insect cells, and cell-free) are employed for plant protein expression, although a plant expression system is often desirable for plant

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    Application of Cadaverine to Inhibit Biotin Biosynthesis in Plants
    尸胺在抑制植物生物素合成中的应用
    作者:Nicole M. Gibbs, Shih-Heng Su and Patrick H. Masson日期:04/20/2022,浏览量:526,Q&A: 0
    [Abstract]

    Biotin is an essential vitamin in plants. However, characterization of biotin deficiency has been limited by embryo lethality in mutants, which can only be rescued by supplementation of biotin. Here, we describe a protocol to characterize biotin deficiency in Arabidopsis thaliana through application of the polyamine cadaverine. Cadaverine

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    In vitro Auto- and Substrate-Ubiquitination Assays
    体外自体和底物泛素化测定
    作者:Hye Lin Park, Han Yong Lee and Gyeong Mee Yoon日期:04/05/2022,浏览量:604,Q&A: 0
    [Abstract]

    The precise regulation of the homeostasis of the cellular proteome is critical for the appropriate growth and development of plants. It also allows the plants to respond to various environmental stresses, by modulating their biochemical and physiological aspects in a timely manner. Ubiquitination of cellular proteins is one of the major

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    Activity-based Protein Profiling of Serine Hydrolase Superfamily Enzymes
    丝氨酸水解酶超家族酶的基于活性的蛋白质分析
    作者:Achintya Kumar Dolui, Mahadev Latha and Panneerselvam Vijayaraj日期:03/20/2022,浏览量:646,Q&A: 0
    [Abstract]

    Activity-based protein profiling (ABPP) is a chemoproteomics platform to assess the functional state of enzymes in complex biological systems. Over the two decades, ABPP has emerged from a gel-based to gel-free platform, for in-depth proteome analysis with enhanced resolution, sensitivity for target detection, and discovery of small molecule

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