免疫学

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    现刊
    Protocol for High Throughput Screening of Antibody Phage Libraries
    抗体噬菌体文库的高通量筛选方案

    Phage display is a proven and widely used technology for selecting specific antibodies against desired targets. However, an immense amount of effort is required to identify and screen the desired positive clones from

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    Protocol for High Throughput Screening of Antibody Phage Libraries
    抗体噬菌体文库的高通量筛选方案
    [Abstract]

    Phage display is a proven and widely used technology for selecting specific antibodies against desired targets. However, an immense amount of effort is required to identify and screen the desired positive clones from large and diverse combinatorial libraries. On the other hand, the selection of positive binding clones

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    Assessing the Presence of Hematopoietic Stem and Progenitor Cells in Mouse Spleen
    评估小鼠脾脏中造血干细胞和祖细胞的存在
    作者:Isabelle J. Marié, Lara Brambilla and David E. Levy日期:06/05/2022,浏览量:644,Q&A: 0
    [Abstract]

    Transplantation of hematopoietic material into recipient mice is an assay routinely used to determine the presence and function of hematopoietic stem and progenitor cells (HSPCs) in vivo. The principle of the method is to transplant donor cells being tested for HSPCs into a recipient mouse following bone marrow ablation and testing for

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    Protocol to Isolate Germinal Centers by Laser Microdissection
    通过激光显微切割分离生发中心的方法
    作者:Farbod Bahreini, Markus Niebuhr, Julia Belde, Jürgen Westermann and Kathrin Kalies日期:06/05/2022,浏览量:503,Q&A: 0
    [Abstract]

    During adaptive immune responses, germinal centers (GC) appear as transient microstructures, in which antigen-specific B and T cells interact with each other. Because only the antigen-activated B and T cells, such as Plasmablasts or follicular T helper (Tfh) cells, are present in GC, the in depth-analysis of GC is of great interest. To identify

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    Flow Cytometric Characterization of Macrophages Infected in vitro with Salmonella enterica Serovar Typhimurium Expressing Red Fluorescent Protein
    表达红色荧光蛋白的肠沙门氏菌血清鼠伤寒菌体外感染巨噬细胞的流式细胞术研究
    作者:Natascha Brigo, Christa Pfeifhofer-Obermair, Egon Demetz, Piotr Tymoszuk and Günter Weiss日期:06/05/2022,浏览量:431,Q&A: 0
    [Abstract]

    Macrophages are important for host defense against intracellular pathogens like Salmonella and can be differentiated into two major subtypes. M1 macrophages, which are pro-inflammatory and induce antimicrobial immune effector mechanisms, including the expression of inducible nitric oxide synthase (iNOS), and M2 macrophages, which exert

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    Protocol to Induce Follicular T Helper Cells, Germinal Centers, and Skin Lesions in Mouse Models for Skin Blistering Diseases
    在皮肤起泡疾病小鼠模型中诱导滤泡 T 辅助细胞、生发中心和皮肤损伤的方法
    作者:Farbod Bahreini, Markus Niebuhr, Julia Belde, Katja Bieber, Juergen Westermann and Kathrin Kalies日期:05/20/2022,浏览量:846,Q&A: 0
    [Abstract]

    Autoreactive T cells in autoantibody-mediated autoimmune diseases can be divided into two major subsets: (i) follicular T helper cells (Tfh) that provide T cell help in germinal centers (GC) and (ii) effector T (Teff) cells that immigrate into peripheral tissue sites such as the skin and mediate local inflammation. To study the sequence of events

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    Characterization of the Elasticity of CD4+ T Cells: An Approach Based on Peak Force Quantitative Nanomechanical Mapping
    CD4+T细胞弹性的特征:一个基于峰值力定量纳米力学图的方法
    作者:Philipp Jung, Xiangda Zhou, Sandra Iden, Bin Qu and Markus Bischoff日期:04/20/2022,浏览量:899,Q&A: 0
    [Abstract]

    CD4+ T cells are essential players in orchestrating the specific immune response against intracellular pathogens, and in inhibiting tumor development in an early stage. The activation of T cells is triggered by engagement of T cell receptors (TCRs). Here, CD3 and CD28 molecules are key factors, (co)stimulating signaling pathways essential for

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    An Optimized Tat/Rev Induced Limiting Dilution Assay for the Characterization of HIV-1 Latent Reservoirs
    一种优化的 Tat/Rev 诱导的限制稀释测定表征 HIV-1 潜伏储库
    [Abstract]

    The administration of antiretroviral therapy (ART) leads to a rapid reduction in plasma viral load in HIV-1 seropositive subjects. However, when ART is suspended, the virus rebounds due to the presence of a latent viral reservoir. Several techniques have been developed to characterize this latent viral reservoir. Of the various assay formats

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    Identification of SARS-CoV-2 Neutralizing Antibody with Pseudotyped Virus-based Test on HEK-293T hACE2 Cells
    基于 HEK-293T hACE2 细胞的假型病毒试验鉴定 SARS-CoV-2 中和抗体
    作者:Constant Gillot, Julien Favresse, Vincent Maloteau, Jean-Michel Dogné and Jonathan Douxfils日期:04/05/2022,浏览量:552,Q&A: 0
    [Abstract]

    Neutralizing antibodies (NAbs) are of particular importance because they can prevent binding of the receptor binding domain (RBD) of the spike protein (S protein) to the angiotensin-converting enzyme 2 (ACE2) receptor present at the surface of human cells, preventing virus entry into the host cells. The gold standard method for detection of

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    A Mouse Infection Model with a Wildtype Salmonella enterica Serovar Typhimurium Strain for the Analysis of Inflammatory Innate Immune Cells
    用于分析炎性先天免疫细胞的野生型肠沙门氏菌鼠伤寒血清型小鼠感染模型
    作者:Christa Pfeifhofer-Obermair, Natascha Brigo, Piotr Tymoszuk and Günter Weiss日期:04/05/2022,浏览量:502,Q&A: 0
    [Abstract]

    Salmonella enterica serovar Typhimurium (S. Typhimurium) is a Gram-negative, facultative intracellular bacterium, which causes gastrointestinal disorders in humans, and systemic, typhoid fever-like infections in mice. Our current knowledge regarding the involvement of cellular and humoral immunity in the defense from S. Typhimurium infections is

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    Differentiation of Human Induced Pluripotent Stem Cell into Macrophages
    人诱导多能干细胞向巨噬细胞的分化
    作者:Harriet Douthwaite, Aitor Bermejo Arteagabeitia and Subhankar Mukhopadhyay日期:03/20/2022,浏览量:1035,Q&A: 0
    [Abstract]

    As a model to interrogate human macrophage biology, macrophages differentiated from human induced pluripotent stem cells (hiPSCs) transcend other existing models by circumventing the variability seen in human monocyte-derived macrophages, whilst epitomizing macrophage phenotypic and functional characteristics over those offered by macrophage-like

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