分子生物学

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    现刊
    CRISPR/Cas9-mediated Gene Knockout Followed by Negative Selection Leads to a Complete TCR Depletion in orthoCAR19 T Cells
    CRISPR/Cas9 介导的基因敲除后负选择导致原CAR19 T 细胞中的 TCR 完全耗尽
    作者:Qian Zhang, Jingyi Yang, Eric Nigel Ebenezer Anand Manoharan, Alvin B. Yu and Michael C. Milone日期:08/05/2022,浏览量:229,Q&A: 0

    Genome-editing technologies, especially CRISPR (clustered regularly interspaced short palindrome repeats)/Cas9 (CRISPR-associated protein 9), endows researchers the ability to make efficient, simple, and precise genomic DNA changes in many

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    DSP-crosslinking and Immunoprecipitation to Isolate Weak Protein Complex
    DSP交联和免疫沉淀分离弱蛋白复合物
    作者:Kotaro Akaki, Takashi Mino and Osamu Takeuchi日期:08/05/2022,浏览量:144,Q&A: 0

    Detecting protein-protein interactions (PPIs) is one of the most used approaches to reveal the molecular regulation of protein of interests (POIs). Immunoprecipitation of POIs followed by mass spectrometry or western blot analysis enables us to

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    CRISPR/Cas9-mediated Gene Knockout Followed by Negative Selection Leads to a Complete TCR Depletion in orthoCAR19 T Cells
    CRISPR/Cas9 介导的基因敲除后负选择导致原CAR19 T 细胞中的 TCR 完全耗尽
    作者:Qian Zhang, Jingyi Yang, Eric Nigel Ebenezer Anand Manoharan, Alvin B. Yu and Michael C. Milone日期:08/05/2022,浏览量:229,Q&A: 0
    [Abstract]

    Genome-editing technologies, especially CRISPR (clustered regularly interspaced short palindrome repeats)/Cas9 (CRISPR-associated protein 9), endows researchers the ability to make efficient, simple, and precise genomic DNA changes in many eukaryotic cell types. CRISPR/Cas9-mediated efficient gene knockout holds huge potential to improve the

    ...
    DSP-crosslinking and Immunoprecipitation to Isolate Weak Protein Complex
    DSP交联和免疫沉淀分离弱蛋白复合物
    作者:Kotaro Akaki, Takashi Mino and Osamu Takeuchi日期:08/05/2022,浏览量:144,Q&A: 0
    [Abstract]

    Detecting protein-protein interactions (PPIs) is one of the most used approaches to reveal the molecular regulation of protein of interests (POIs). Immunoprecipitation of POIs followed by mass spectrometry or western blot analysis enables us to detect co-precipitated POI-binding proteins. However, some binding proteins are lost during cell lysis

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    Purification and Immunostaining of Mouse Ependymal Ciliary Shafts
    小鼠室管膜纤毛干的纯化和免疫染色
    作者:Kai Hao, Xueliang Zhu and Xiumin Yan日期:07/20/2022,浏览量:852,Q&A: 0
    [Abstract]

    Cilia and flagella are microtubule-based hair-like organelles protruding from the surface of most eukaryotic cells, and play essential roles in cell locomotion, left-right asymmetry, embryo development, and tissue homeostasis. With isolated cilia and flagella, great progress has been made in understanding the composition, structure, and function

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    Gene Expression Analysis in Stem Cell-derived Cortical Neuronal Cultures Using Multi-well SYBR Green Quantitative PCR Arrays
    使用多孔 SYBR Green 定量 PCR 阵列在干细胞衍生的皮质神经元培养物中进行基因表达分析
    作者:Vasavi Nallur Srinivasaraghavan, Faria Zafar and Birgitt Schüle日期:07/20/2022,浏览量:525,Q&A: 0
    [Abstract]

    To optimize differentiation protocols for stem cell-based in vitro modeling applications, it is essential to assess the change in gene expression during the differentiation process. This allows controlling its differentiation efficiency into the target cell types. While RNA transcriptomics provides detail at a larger scale, timing and cost are

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    VirScan: High-throughput Profiling of Antiviral Antibody Epitopes
    Vir Scan: 抗病毒抗体表位的高通量分析
    作者:Ellen L. Shrock, Christine L. Shrock and Stephen J. Elledge日期:07/05/2022,浏览量:1065,Q&A: 0
    [Abstract]

    Profiling the specificities of antibodies can reveal a wealth of information about humoral immune responses and the antigens they target. Here, we present a protocol for VirScan, an application of the phage immunoprecipitation sequencing (PhIP-Seq) method for profiling the specificities of human antiviral antibodies. Accompanying this protocol is

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    HaloChIP-seq for Antibody-Independent Mapping of Mouse Transcription Factor Cistromes in vivo
    HaloChIP-seq用于体内小鼠转录因子 Cistromes 的抗体独立映射
    [Abstract]

    Chromatin immunoprecipitation (ChIP) maps, on a genome-wide scale, transcription factor binding sites, and the distribution of other chromatin-associated proteins and their modifications. As such, it provides valuable insights into mechanisms of gene regulation. However, successful ChIP experiments are dependent on the availability of a

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    Experimental Models for Cold Exposure of Muscle in vitro and in vivo
    体外和体内肌肉低温暴露实验模型
    作者:Tiril Schjølberg, Lucia Asoawe, Solveig Krapf, Arild C. Rustan, G. Hege Thoresen and Fred Haugen日期:07/05/2022,浏览量:916,Q&A: 0
    [Abstract]

    Work in cold environments may have a significant impact on occupational health. In these and similar situations, cold exposure localized to the extremities may reduce the temperature of underlying tissues. To investigate the molecular effects of cold exposure in muscle, and since adequate methods were missing, we established two experimental cold

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    ATAC-Seq of a Single Myofiber from Mus musculus
    小家鼠单一肌纤维的ATAC-Seq
    作者:Korin Sahinyan, Darren M. Blackburn and Vahab D. Soleimani日期:06/20/2022,浏览量:1515,Q&A: 0
    [Abstract]

    Chromatin accessibility is a key determinant of gene expression that can be altered under different physiological and disease conditions. Skeletal muscle is made up of myofibers that are highly plastic and adaptive. Therefore, assessing the genome-wide chromatin state of myofibers under various conditions is very important to gain insight into the

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    A Modified Fluctuation Assay with a CAN1 Reporter in Yeast
    酵母中CAN1报告基因的改良波动测定
    作者:Pengyao Jiang, Anja R. Ollodart and Maitreya J. Dunham日期:06/05/2022,浏览量:700,Q&A: 0
    [Abstract]

    Understanding the generation of mutations is fundamental to understanding evolution and genetic disease; however, the rarity of such events makes experimentally identifying them difficult. Mutation accumulation (MA) methods have been widely used. MA lines require serial bottlenecks to fix de novo mutations, followed by whole-genome sequencing.

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    A Highly Sensitive Method to Efficiently Profile the Histone Modifications of FFPE Samples
    一种高效分析 FFPE 样品组蛋白修饰的高灵敏度方法
    作者:Linxuan Zhao, Vamsi Krishna Polavarapu, Ram Prakash Yadav, Pengwei Xing and Xingqi Chen日期:05/20/2022,浏览量:998,Q&A: 0
    [Abstract]

    The majority of biopsies in both basic research and translational cancer studies are preserved in the format of archived formalin-fixed paraffin-embedded (FFPE) samples. Profiling histone modifications in archived FFPE tissues is critically important to understand gene regulation in human disease. The required input for current genome-wide

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