癌症生物学

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    现刊
    Plasma Membrane Wounding and Repair Assays for Eukaryotic Cells
    真核细胞的等离子膜损伤和修复试验
    [Abstract]

    Damage to the plasma membrane and loss of membrane integrity are detrimental to eukaryotic cells. It is, therefore, essential that cells possess an efficient membrane repair system to survive. However, the different cellular and molecular mechanisms behind plasma membrane repair have not been fully elucidated. Here, we present three complementary

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    A Highly Sensitive Method to Efficiently Profile the Histone Modifications of FFPE Samples
    一种高效分析 FFPE 样品组蛋白修饰的高灵敏度方法
    作者:Linxuan Zhao, Vamsi Krishna Polavarapu, Ram Prakash Yadav, Pengwei Xing and Xingqi Chen日期:05/20/2022,浏览量:741,Q&A: 0
    [Abstract]

    The majority of biopsies in both basic research and translational cancer studies are preserved in the format of archived formalin-fixed paraffin-embedded (FFPE) samples. Profiling histone modifications in archived FFPE tissues is critically important to understand gene regulation in human disease. The required input for current genome-wide

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    A Flow Cytometry-Based Method for Analyzing DNA End Resection in G0- and G1-Phase Mammalian Cells
    一种基于流式细胞术的分析 G0和G1期哺乳动物细胞中 DNA 末端切除的方法
    作者:Bo-Ruei Chen, Jessica K. Tyler and Barry P. Sleckman日期:05/20/2022,浏览量:798,Q&A: 0
    [Abstract]

    DNA double strand breaks (DSBs) constantly arise in cells during normal cellular processes or upon exposure to genotoxic agents, and are repaired mostly by homologous recombination (HR) and non-homologous end joining (NHEJ). One key determinant of DNA DSB repair pathway choice is the processing of broken DNA ends to generate single strand DNA

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    Measurement of Cell Intrinsic TGF-β Activation Mediated by the Integrin αvβ8
    整合素αvβ8介导的细胞固有TGF-β活化测定
    作者:Robert Ian Seed and Stephen Lloyd Nishimura日期:04/20/2022,浏览量:1220,Q&A: 0
    [Abstract]

    Transforming growth factor beta (TGF-β) is a multi-functional cytokine that plays a significant role in multiple diseases, including fibrosis and tumor progression. Whilst the biologic effects of TGF-β are well characterized, it is unclear how TGF-β signaling is regulated to impart specific responses within certain cell types. One

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    Low-viscosity Matrix Suspension Culture for Human Colorectal Epithelial Organoids and Tumoroids
    用于人结直肠上皮类器官和类肿瘤的低粘度基质悬浮培养
    作者:Tao Tan, Yumiko Hirokawa, Jordan Clarke, Anuratha Sakthianandeswaren and Oliver M. Sieber日期:04/20/2022,浏览量:992,Q&A: 0
    [Abstract]

    Three-dimensional culture of human normal colorectal epithelium and cancer tissue as organoids and tumoroids has transformed the study of diseases of the large intestine. A widely used strategy for generating patient-derived colorectal organoids and tumoroids involves embedding cells in domes of extracellular matrix (ECM). Despite its success,

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    Conditional Human BRD4 Knock-In Transgenic Mouse Genotyping and Protein Isoform Detection
    条件性人 BRD4 敲入转基因小鼠基因分型和蛋白质异构体检测
    作者:Michael Paul Lewis, Shwu-Yuan Wu and Cheng-Ming Chiang日期:04/05/2022,浏览量:574,Q&A: 0
    [Abstract]

    Bromodomain-containing protein 4 (BRD4) is an acetyl-lysine reader protein and transcriptional regulator implicated in chromatin dynamics and cancer development. Several BRD4 isoforms have been detected in humans with the long isoform (BRD4-L, aa 1-1,362) playing a tumor-suppressive role and a major short isoform (BRD4-S, aa 1-722) having

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    Image-based Quantification of Macropinocytosis Using Dextran Uptake into Cultured Cells
    基于图像定量使用葡聚糖摄取到培养细胞中的巨胞饮
    作者:Anh H. Le and Laura M. Machesky日期:04/05/2022,浏览量:735,Q&A: 0
    [Abstract]

    Macropinocytosis is an evolutionarily conserved process, which is characterized by the formation of membrane ruffles and the uptake of extracellular fluid. We recently demonstrated a role for CYFIP-related Rac1 Interactor (CYRI) proteins in macropinocytosis. High-molecular weight dextran (70kDa or higher) has generally been used as a marker for

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    Single-cell Damagenome Profiling by Linear Copying and Splitting based Whole Genome Amplification (LCS-WGA)
    基于线性复制和分裂的全基因组扩增法(LCS-WGA)分析单细胞损伤组
    作者:Yichi Niu, Qiangyuan Zhu and Chenghang Zong日期:03/20/2022,浏览量:658,Q&A: 0
    [Abstract]

    Spontaneous DNA damage frequently occurs on the human genome, and it could alter gene expression by inducing mutagenesis or epigenetic changes. Therefore, it is highly desired to profile DNA damage distribution on the human genome and identify the genes that are prone to DNA damage. Here, we present a novel single-cell whole-genome amplification

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    3D-Structured Illumination Microscopy of Centrosomes in Human Cell Lines
    人类细胞系中心体的 3D 结构照明显微镜
    作者:Kari-Anne M. Frikstad, Kay O. Schink, Sania Gilani, Lotte B. Pedersen and Sebastian Patzke日期:03/20/2022,浏览量:899,Q&A: 0
    [Abstract]

    The centrosome is the main microtubule-organizing center of animal cells, and is composed of two barrel-shaped microtubule-based centrioles embedded in protein dense pericentriolar material. Compositional and architectural re-organization of the centrosome drives its duplication, and enables its microtubule-organizing activity and capability to

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    Three-dimensional Models of the Nasopharynx for the Study of Epstein-Barr Virus Infection
    研究EB病毒感染的鼻咽三维模型
    [Abstract]

    The ubiquitous and cancer-associated Epstein-Barr virus (EBV) is associated with nearly all cases of nasopharyngeal carcinoma (NPC). Nasopharyngeal tissue is comprised of both pseudostratified and stratified epithelium, which are modeled in three-dimensional (3-D) cell culture. The cellular origin of EBV-associated NPC is as yet unknown, but both

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