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细胞生物学

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    Detection of Intracellular Reduced (Catalytically Active) SHP-1 and Analyses of Catalytically Inactive SHP-1 after Oxidation by Pervanadate or H2O2
    细胞内还原的(具有催化活性的)SHP-1检测以及在经钒酸盐或H2O2氧化后无催化活性SHP-1的分析
    作者:Seeyoung Choi and Paul E. Love日期:01/05/2018,浏览量:174,Q&A: 0
    Oxidative inactivation of cysteine-dependent Protein Tyrosine Phosphatases (PTPs) by cellular reactive oxygen species (ROS) plays a critical role in regulating signal transduction in multiple cell types. The phosphatase activity of most PTPs depends ...
    Electron Tomography to Study the Three-dimensional Structure of Plasmodesmata in Plant Tissues–from High Pressure Freezing Preparation to Ultrathin Section Collection
    采用电子断层摄影术研究植物组织中的胞间连丝的三维结构 — 从高压冷冻制备到超薄切片采集
    作者:William J. Nicolas, Emmanuelle Bayer and Lysiane Brocard日期:01/05/2018,浏览量:186,Q&A: 0
    Plasmodesmata (PD) are nanometric (~20 nm wide) membrane lined pores encased in the cell walls of the adjacent plant cells. They allow the cells to exchange all types of molecules ranging from nutrients like sugar, hormones, to RNAs and various ...
    Micro-computed Tomography to Visualize Vascular Networks in Maize Stems
    玉米茎杆中维管网络的微型计算机断层摄影术观察
    作者:Akiteru Maeno and Katsutoshi Tsuda日期:01/05/2018,浏览量:175,Q&A: 0
    Plant vascular systems in the stem connect roots with aerial organs to move solutes containing minerals, nutrients as well as signaling molecules, and therefore, they play pivotal roles in plant growth and development. However, stem vascular ...
    High Throughput NPY-Venus and Serotonin Secretion Assays for Regulated Exocytosis in Neuroendocrine Cells
    高通量NPY-Venus和5-羟色胺分泌实验分析神经内分泌细胞中被调控的胞吐作用
    作者:Xingmin Aaron Zhang and Thomas F.J. Martin日期:01/05/2018,浏览量:153,Q&A: 0
    Here we describe two assays to measure dense core vesicle (DCV) exocytosis-mediated cargo secretion in neuroendocrine cells. To conduct siRNA screens for novel genes in regulated DCV exocytosis, we developed a plate reader-based secretion assay ...
    The RiboPuromycylation Method (RPM): an Immunofluorescence Technique to Map Translation Sites at the Sub-cellular Level
    RiboPuromycylation方法(RPM):一种在亚细胞水平绘制翻译位点图谱的免疫荧光技术
    作者:Amandine Bastide, Jonathan W. Yewdell and Alexandre David日期:01/05/2018,浏览量:131,Q&A: 0
    While isotopic labeling of amino acids remains the reference method in the field for quantifying translation rate, it does not provide any information on spatial localization of translation sites. The rationale behind developing the ...
    Real-time Analysis of Auxin Response, Cell Wall pH and Elongation in Arabidopsis thaliana Hypocotyls
    拟南芥下胚轴植物生长素响应、细胞壁pH和伸长的实时分析
    作者:Lanxin Li, S. F. Gabriel Krens, Matyáš Fendrych and Jiří Friml日期:01/05/2018,浏览量:268,Q&A: 0
    The rapid auxin-triggered growth of the Arabidopsis hypocotyls involves the nuclear TIR1/AFB-Aux/IAA signaling and is accompanied by acidification of the apoplast and cell walls (Fendrych et al., 2016). Here, we describe in detail ...
    A Rodent Model for Chronic Brain Hypoperfusion Related Diseases: Permanent Bilateral Occlusion of the Common Carotid Arteries (2VO) in Rats
    慢性脑灌注不足相关疾病的啮齿动物模型:大鼠双侧颈总动脉永久性闭塞(2VO)
    作者:Mei-Ling Yan and Jing Ai日期:01/05/2018,浏览量:122,Q&A: 0
    Permanent occlusion of bilateral common carotid arteries (2VO) in rat is considered as a suitable animal model to mimic chronic brain hypoperfusion status, which is proved to be a risk factor to precede the Alzheimer’s disease or/and vascular ...
    Mutant Huntingtin Secretion in Neuro2A Cells and Rat Primary Cortical Neurons
    Neuro2A细胞和大鼠原代皮层神经元中突变亨廷顿蛋白的分泌
    作者:Katarina Trajkovic, Hyunkyung Jeong and Dimitri Krainc日期:01/05/2018,浏览量:111,Q&A: 0
    Quantitative analysis of proteins secreted from the cells poses a challenge due to their low abundance and the interfering presence of a large amount of bovine serum albumin (BSA) in the cell culture media. We established assays for detection of ...
    Bacterial Aggregation Assay in the Presence of Cyclic Lipopeptides
    环脂肽存在情况下细菌聚集实验
    作者:Pengyuan Xiu, Rui Liu, Dechao Zhang and Chaomin Sun日期:01/05/2018,浏览量:83,Q&A: 0
    Lipopeptides is an important class of biosurfactants having antimicrobial and anti-adhesive activity against pathogenic bacteria. These include surfactin, fengycin, iturin, bacillomycin, mycosubtilin, lichenysin, and pumilacidin (Arima et al. ...
    Detection of Intracellular Reduced (Catalytically Active) SHP-1 and Analyses of Catalytically Inactive SHP-1 after Oxidation by Pervanadate or H2O2
    细胞内还原的(具有催化活性的)SHP-1检测以及在经钒酸盐或H2O2氧化后无催化活性SHP-1的分析
    作者:Seeyoung Choi and Paul E. Love日期:01/05/2018,浏览量:174,Q&A: 0
    [Abstract] Oxidative inactivation of cysteine-dependent Protein Tyrosine Phosphatases (PTPs) by cellular reactive oxygen species (ROS) plays a critical role in regulating signal transduction in multiple cell types. The phosphatase activity of most PTPs depends upon a ‘signature’ cysteine residue within the catalytic domain that is maintained in the ...
    Electron Tomography to Study the Three-dimensional Structure of Plasmodesmata in Plant Tissues–from High Pressure Freezing Preparation to Ultrathin Section Collection
    采用电子断层摄影术研究植物组织中的胞间连丝的三维结构 — 从高压冷冻制备到超薄切片采集
    作者:William J. Nicolas, Emmanuelle Bayer and Lysiane Brocard日期:01/05/2018,浏览量:186,Q&A: 0
    [Abstract] Plasmodesmata (PD) are nanometric (~20 nm wide) membrane lined pores encased in the cell walls of the adjacent plant cells. They allow the cells to exchange all types of molecules ranging from nutrients like sugar, hormones, to RNAs and various proteins. Unfortunately, they are also hijacked by phyto-viruses, enabling them to spread from ...
    Micro-computed Tomography to Visualize Vascular Networks in Maize Stems
    玉米茎杆中维管网络的微型计算机断层摄影术观察
    作者:Akiteru Maeno and Katsutoshi Tsuda日期:01/05/2018,浏览量:175,Q&A: 0
    [Abstract] Plant vascular systems in the stem connect roots with aerial organs to move solutes containing minerals, nutrients as well as signaling molecules, and therefore, they play pivotal roles in plant growth and development. However, stem vascular systems, especially in crop species, have been poorly described since they are deeply embedded in the ...
    High Throughput NPY-Venus and Serotonin Secretion Assays for Regulated Exocytosis in Neuroendocrine Cells
    高通量NPY-Venus和5-羟色胺分泌实验分析神经内分泌细胞中被调控的胞吐作用
    作者:Xingmin Aaron Zhang and Thomas F.J. Martin日期:01/05/2018,浏览量:153,Q&A: 0
    [Abstract] Here we describe two assays to measure dense core vesicle (DCV) exocytosis-mediated cargo secretion in neuroendocrine cells. To conduct siRNA screens for novel genes in regulated DCV exocytosis, we developed a plate reader-based secretion assay using DCV cargo, NPY-Venus, and an orthogonal 3H-serotonin secretion assay. The NPY-Venus ...
    The RiboPuromycylation Method (RPM): an Immunofluorescence Technique to Map Translation Sites at the Sub-cellular Level
    RiboPuromycylation方法(RPM):一种在亚细胞水平绘制翻译位点图谱的免疫荧光技术
    作者:Amandine Bastide, Jonathan W. Yewdell and Alexandre David日期:01/05/2018,浏览量:131,Q&A: 0
    [Abstract] While isotopic labeling of amino acids remains the reference method in the field for quantifying translation rate, it does not provide any information on spatial localization of translation sites. The rationale behind developing the ribopuromycylation method (RPM) was primarily to map translation sites at the sub-cellular level while avoiding ...
    Real-time Analysis of Auxin Response, Cell Wall pH and Elongation in Arabidopsis thaliana Hypocotyls
    拟南芥下胚轴植物生长素响应、细胞壁pH和伸长的实时分析
    作者:Lanxin Li, S. F. Gabriel Krens, Matyáš Fendrych and Jiří Friml日期:01/05/2018,浏览量:268,Q&A: 0
    [Abstract] The rapid auxin-triggered growth of the Arabidopsis hypocotyls involves the nuclear TIR1/AFB-Aux/IAA signaling and is accompanied by acidification of the apoplast and cell walls (Fendrych et al., 2016). Here, we describe in detail the method for analysis of the elongation and the TIR1/AFB-Aux/IAA-dependent auxin response in ...
    A Rodent Model for Chronic Brain Hypoperfusion Related Diseases: Permanent Bilateral Occlusion of the Common Carotid Arteries (2VO) in Rats
    慢性脑灌注不足相关疾病的啮齿动物模型:大鼠双侧颈总动脉永久性闭塞(2VO)
    作者:Mei-Ling Yan and Jing Ai日期:01/05/2018,浏览量:122,Q&A: 0
    [Abstract] Permanent occlusion of bilateral common carotid arteries (2VO) in rat is considered as a suitable animal model to mimic chronic brain hypoperfusion status, which is proved to be a risk factor to precede the Alzheimer’s disease or/and vascular dementia. In this protocol, we describe how to successfully ligate the bilateral common carotid arteries ...
    Mutant Huntingtin Secretion in Neuro2A Cells and Rat Primary Cortical Neurons
    Neuro2A细胞和大鼠原代皮层神经元中突变亨廷顿蛋白的分泌
    作者:Katarina Trajkovic, Hyunkyung Jeong and Dimitri Krainc日期:01/05/2018,浏览量:111,Q&A: 0
    [Abstract] Quantitative analysis of proteins secreted from the cells poses a challenge due to their low abundance and the interfering presence of a large amount of bovine serum albumin (BSA) in the cell culture media. We established assays for detection of mutant huntingtin (mHtt) secreted from Neuro2A cell line stably expressing mHtt and rat primary ...
    Bacterial Aggregation Assay in the Presence of Cyclic Lipopeptides
    环脂肽存在情况下细菌聚集实验
    作者:Pengyuan Xiu, Rui Liu, Dechao Zhang and Chaomin Sun日期:01/05/2018,浏览量:83,Q&A: 0
    [Abstract] Lipopeptides is an important class of biosurfactants having antimicrobial and anti-adhesive activity against pathogenic bacteria. These include surfactin, fengycin, iturin, bacillomycin, mycosubtilin, lichenysin, and pumilacidin (Arima et al., 1968; Naruse et al., 1990; Yakimov et al., 1995; Steller and Vater, 2000; ...
    Preparation of Onion Epidermal Cell Walls for Imaging by Atomic Force Microscopy (AFM)
    制备洋葱表皮细胞壁用于原子力显微镜(AFM)成像
    作者:Tian Zhang and Daniel J. Cosgrove日期:12/20/2017,浏览量:421,Q&A: 0
    [Abstract] The growing plant cell wall is comprised of long, thin cellulose microfibrils embedded in a hydrated matrix of polysaccharides and glycoproteins. These components are typically constructed in layers (lamellae) on the inner surface of the cell wall, i.e., between the existing wall and the plasma membrane. The organization of these ...