细胞生物学

分类

    现刊
    Isolation of Pure Mitochondria from Rat Kidneys and Western Blot of Mitochondrial Respiratory Chain Complexes
    大鼠肾脏单纯线粒体的分离和线粒体呼吸链复合物蛋白质免疫印迹
    Cardiac, neuronal and renal tubular epithelial cells are the most metabolically active cells in the body. Their fate depends largely on their mitochondria as the primary energy generating system which participates in the control of apoptosis, cell ...
    Antisense Oligodeoxynucleotide Perfusion Blocks Gene Expression of Synaptic Plasticity-related Proteins without Inducing Compensation in Hippocampal Slices
    反义寡脱氧核苷酸灌注可阻突触可塑性相关蛋白在海马切片上的基因表达且无诱导补偿作用
    The elucidation of the molecular mechanisms of long-term synaptic plasticity has been hindered by both the compensation that can occur after chronic loss of the core plasticity molecules and by ex vivo conditions that may not reproduce in ...
    3D Organoid Formation from the Murine Salivary Gland Cell Line SIMS
    小鼠唾液腺SIMS细胞系的三维类器官形成
    作者:Harleen K. Athwal and Isabelle M. A. Lombaert日期:10/05/2019,浏览量:147,Q&A: 0
    Salivary glands consist of multiple phenotypically and functionally unique cell populations, such as the acinar, ductal, and myoepithelial cells that help produce, modify, and secrete saliva (Lombaert et al., 2011). Identification of ...
    Reconstituting Breast Tissue with Organotypic Three-dimensional Co-culture of Epithelial and Stromal Cells in Discontinuous Extracellular Matrices
    通过不连续细胞外基质中上皮细胞和基质细胞三维共培养重建乳腺组织
    作者:Gang Ren, Vandana Sharma, Joshua Letson, Yashna Walia, Veani Fernando and Saori Furuta日期:10/05/2019,浏览量:200,Q&A: 0
    Co-culture systems utilizing reconstituted or synthetic extracellular matrix (ECM) and micropatterning techniques have enabled the reconstruction of surface epithelial tissues. This technique has been utilized in the regeneration, disease modeling ...
    Measurement of Acid Ecto-phosphatase Activity in Live Leishmania donovani Parasites
    杜氏利什曼原虫中酸性胞外磷酸酶活性的活体检测
    作者:Amalia Papadaki and Haralabia Boleti日期:10/05/2019,浏览量:113,Q&A: 0
    Acid ecto-phosphatases are enzymes that hydrolyze phosphomonoesters in the acidic pH range with their active sites facing the extacellular medium. Their activities can be measured in living cells. In bacteria and protozoan pathogens, acid ...
    Imaging the Vasculature of Immunodeficient Mice Using Positron Emission Tomography/Computed Tomography (PET/CT) and 18F-fluorodeoxyglucose Labeled Human Erythrocytes
    利用正电子发射断层扫描/计算机断层扫描(PET、CT)和18F-氟脱氧葡萄糖标记人类红细胞 进行免疫缺陷小鼠脉管系统成像
    作者:Shaowei Wang and Jung W. Choi日期:10/05/2019,浏览量:111,Q&A: 0
    Nuclear blood pool imaging using radiolabeled red blood cells has been used in the clinical setting for the evaluation of a number of medical conditions including gastrointestinal hemorrhage, impaired cardiac contractility, and altered ...
    A Novel Protocol to Generate Decellularized Bovine Spinal Cord Extracellular Matrix-based Scaffolds (3D-dCBS)
    一种获得去细胞牛脊髓细胞外基质构成支架的新方法
    作者:Yavuz E. Arslan, Burcu Efe and Tugba Sezgin Arslan日期:10/05/2019,浏览量:144,Q&A: 0
    Extracellular matrix (ECM)-based tissue engineering scaffolds have an essential role in promoting tissue regeneration. Nerve tissue engineering aims at facilitating the repair of permanent damage to the peripheral and central nervous systems, which ...
    Isolation of Pure Mitochondria from Rat Kidneys and Western Blot of Mitochondrial Respiratory Chain Complexes
    大鼠肾脏单纯线粒体的分离和线粒体呼吸链复合物蛋白质免疫印迹
    [Abstract] Cardiac, neuronal and renal tubular epithelial cells are the most metabolically active cells in the body. Their fate depends largely on their mitochondria as the primary energy generating system which participates in the control of apoptosis, cell cycle and metabolism. Thus, mitochondrial dysfunction is a hallmark of many chronic diseases ...
    Antisense Oligodeoxynucleotide Perfusion Blocks Gene Expression of Synaptic Plasticity-related Proteins without Inducing Compensation in Hippocampal Slices
    反义寡脱氧核苷酸灌注可阻突触可塑性相关蛋白在海马切片上的基因表达且无诱导补偿作用
    [Abstract] The elucidation of the molecular mechanisms of long-term synaptic plasticity has been hindered by both the compensation that can occur after chronic loss of the core plasticity molecules and by ex vivo conditions that may not reproduce in vivo plasticity. Here we describe a novel method to rapidly suppress gene expression by ...
    3D Organoid Formation from the Murine Salivary Gland Cell Line SIMS
    小鼠唾液腺SIMS细胞系的三维类器官形成
    作者:Harleen K. Athwal and Isabelle M. A. Lombaert日期:10/05/2019,浏览量:147,Q&A: 0
    [Abstract] Salivary glands consist of multiple phenotypically and functionally unique cell populations, such as the acinar, ductal, and myoepithelial cells that help produce, modify, and secrete saliva (Lombaert et al., 2011). Identification of mechanisms and factors that regulate these populations has been of key interest, as salivary gland-related ...
    Reconstituting Breast Tissue with Organotypic Three-dimensional Co-culture of Epithelial and Stromal Cells in Discontinuous Extracellular Matrices
    通过不连续细胞外基质中上皮细胞和基质细胞三维共培养重建乳腺组织
    作者:Gang Ren, Vandana Sharma, Joshua Letson, Yashna Walia, Veani Fernando and Saori Furuta日期:10/05/2019,浏览量:200,Q&A: 0
    [Abstract] Co-culture systems utilizing reconstituted or synthetic extracellular matrix (ECM) and micropatterning techniques have enabled the reconstruction of surface epithelial tissues. This technique has been utilized in the regeneration, disease modeling and drug screening of the surface epithelia, such as the skin and esophagus. On the other hand, the ...
    Measurement of Acid Ecto-phosphatase Activity in Live Leishmania donovani Parasites
    杜氏利什曼原虫中酸性胞外磷酸酶活性的活体检测
    作者:Amalia Papadaki and Haralabia Boleti日期:10/05/2019,浏览量:113,Q&A: 0
    [Abstract] Acid ecto-phosphatases are enzymes that hydrolyze phosphomonoesters in the acidic pH range with their active sites facing the extacellular medium. Their activities can be measured in living cells. In bacteria and protozoan pathogens, acid ecto-phosphatases have been associated with the survival of intracellular pathogens within phagocytes through ...
    Imaging the Vasculature of Immunodeficient Mice Using Positron Emission Tomography/Computed Tomography (PET/CT) and 18F-fluorodeoxyglucose Labeled Human Erythrocytes
    利用正电子发射断层扫描/计算机断层扫描(PET、CT)和18F-氟脱氧葡萄糖标记人类红细胞 进行免疫缺陷小鼠脉管系统成像
    作者:Shaowei Wang and Jung W. Choi日期:10/05/2019,浏览量:111,Q&A: 0
    [Abstract] Nuclear blood pool imaging using radiolabeled red blood cells has been used in the clinical setting for the evaluation of a number of medical conditions including gastrointestinal hemorrhage, impaired cardiac contractility, and altered cerebrovascular blood flow. Nuclear blood pool imaging is typically performed using Technetium-99m-labeled (99m ...
    A Novel Protocol to Generate Decellularized Bovine Spinal Cord Extracellular Matrix-based Scaffolds (3D-dCBS)
    一种获得去细胞牛脊髓细胞外基质构成支架的新方法
    作者:Yavuz E. Arslan, Burcu Efe and Tugba Sezgin Arslan日期:10/05/2019,浏览量:144,Q&A: 0
    [Abstract] Extracellular matrix (ECM)-based tissue engineering scaffolds have an essential role in promoting tissue regeneration. Nerve tissue engineering aims at facilitating the repair of permanent damage to the peripheral and central nervous systems, which are difficult to heal. For this purpose, a variety of biomaterials are being developed consisting of ...
    Quantitation of TLR4 Internalization in Response to LPS in Thioglycollate Elicited Peritoneal Mouse Macrophages by Flow Cytometry
    利用流式细胞术对巯基乙醇酸盐诱导的小鼠腹腔巨噬细胞中LPS-TLR4复合物内化的定量分析
    作者:Katharina Richard, Stefanie N. Vogel and Darren J. Perkins日期:09/20/2019,浏览量:207,Q&A: 0
    [Abstract] Internalization of cell surface Toll-like Receptor 4 (TLR4) is a convenient read-out to measure LPS dependent activation of the TRIF adaptor pathway. We here provide a protocol to quantify the LPS dependent internalization of TLR4 using thioglycollate-elicited peritoneal macrophages by flow cytometry.
    Conjugation of Fab’ Fragments with Fluorescent Dyes for Single-molecule Tracking on Live Cells
    Fab片段结合荧光染料用于活细胞单分子示踪
    作者:I-Ting Teng, Xiangning Bu and Inhee Chung日期:09/20/2019,浏览量:231,Q&A: 0
    [Abstract] Our understanding of the regulation and functions of cell-surface proteins has progressed rapidly with the advent of advanced optical imaging techniques. In particular, single-molecule tracking (SMT) using bright fluorophores conjugated to antibodies and wide-field microscopy methods such as total internal reflection fluorescence microscopy have ...
    SIRF: A Single-cell Assay for in situ Protein Interaction with Nascent DNA Replication Forks
    SIRF:一种蛋白与新生DNA复制叉原位相互作用的单细胞检测办法
    作者:Sunetra Roy and Katharina Schlacher日期:09/20/2019,浏览量:299,Q&A: 0
    [Abstract] The duplication of DNA is a fundamental process that is required for the transfer of the genetic information from parent to daughter cells. Aberrant DNA replication processes are associated with diverse disease phenotypes, including developmental defects, ageing disorders, blood disorders such as Fanconi Anemia, increased inflammation and cancer. ...