细胞生物学

分类

    现刊
    Isolation and Culturing Primary Cholangiocytes from Mouse Liver
    小鼠肝原代胆管细胞的分离与培养

    Cholangiocytes are epithelial cells lining the intrahepatic and extrahepatic bile ducts. Cholangiocytes perform key physiological functions in the liver. Bile synthesized by hepatocytes is secreted into bile canaliculi, further stored in the

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    Collection of in vivo Capacitated Sperm from Different Locations Along the Reproductive Tract of Time-Mated Female Mice by Microdissection
    定时交配的雌性小鼠生殖道不同位置的体内充能精子的显微解剖收集
    作者:Lukas Ded and Jean-Ju Chung日期:10/20/2021,浏览量:133,Q&A: 0

    Mammalian sperm cells are not capable of fertilizing an egg immediately after ejaculation; instead, they must gradually acquire the capacity to fertilize while they travel inside the female reproductive tract. Sperm cells are transported by the

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    Whole-mount Immunohistochemistry to Visualize Mouse Embryonic Dermal Lymphatic Vasculature
    小鼠胚胎真皮淋巴血管的免疫组织化学观察
    作者:Xin Geng and R. Sathish Srinivasan日期:10/20/2021,浏览量:112,Q&A: 0

    Lymphatic vessels are abundant in the skin where they regulate interstitial fluid uptake and immune surveillance. Defects in dermal lymphatic vessels, such as fewer vessels and abnormal lymphatic vessel coverage with mural cells, are frequently

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    Simultaneous Monitoring Cytoplasmic Calcium Ion and Cell Surface Phosphatidylserine in the Necrotic Touch Neurons of Caenorhabditis elegans
    秀丽隐杆线虫坏死触觉神经元胞质钙离子和细胞表面磷脂酰丝氨酸的同时监测
    作者:Yoshitaka Furuta and Zheng Zhou日期:10/20/2021,浏览量:116,Q&A: 0

    Calcium ions trigger many cellular events, including the release of neurotransmitters at the synaptic terminal and excitotoxic cell death. Recently, we have discovered that a transient increase in the level of cytoplasmic Ca2+ triggers the exposure

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    Isolation and Electrophysiology of Murine Sympathetic Postganglionic Neurons in the Thoracic Paravertebral Ganglia
    小鼠胸椎旁神经节交感神经节后神经元的分离与电生理研究
    作者:Mallika Halder, Michael Lee McKinnon, Yaqing Li, Peter Wenner and Shawn Hochman日期:10/20/2021,浏览量:101,Q&A: 0

    The thoracic paravertebral sympathetic chain postganglionic neurons (tSPNs) represent the predominant sympathetic control of vascular function in the trunk and upper extremities. tSPNs cluster to form ganglia linked by an interganglionic nerve and

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    Isolation and Culturing Primary Cholangiocytes from Mouse Liver
    小鼠肝原代胆管细胞的分离与培养
    [Abstract]

    Cholangiocytes are epithelial cells lining the intrahepatic and extrahepatic bile ducts. Cholangiocytes perform key physiological functions in the liver. Bile synthesized by hepatocytes is secreted into bile canaliculi, further stored in the gallbladder, and finally discharged into the duodenum. Due to liver injury, biliary epithelial proliferate

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    Collection of in vivo Capacitated Sperm from Different Locations Along the Reproductive Tract of Time-Mated Female Mice by Microdissection
    定时交配的雌性小鼠生殖道不同位置的体内充能精子的显微解剖收集
    作者:Lukas Ded and Jean-Ju Chung日期:10/20/2021,浏览量:133,Q&A: 0
    [Abstract]

    Mammalian sperm cells are not capable of fertilizing an egg immediately after ejaculation; instead, they must gradually acquire the capacity to fertilize while they travel inside the female reproductive tract. Sperm cells are transported by the muscular activity of the myometrium to the utero-tubal junction (UTJ) before entering the oviduct where

    ...
    Whole-mount Immunohistochemistry to Visualize Mouse Embryonic Dermal Lymphatic Vasculature
    小鼠胚胎真皮淋巴血管的免疫组织化学观察
    作者:Xin Geng and R. Sathish Srinivasan日期:10/20/2021,浏览量:112,Q&A: 0
    [Abstract]

    Lymphatic vessels are abundant in the skin where they regulate interstitial fluid uptake and immune surveillance. Defects in dermal lymphatic vessels, such as fewer vessels and abnormal lymphatic vessel coverage with mural cells, are frequently associated with lymphedema and other lymphatic disorders. Whole-mount immunohistochemistry allows the

    ...
    Simultaneous Monitoring Cytoplasmic Calcium Ion and Cell Surface Phosphatidylserine in the Necrotic Touch Neurons of Caenorhabditis elegans
    秀丽隐杆线虫坏死触觉神经元胞质钙离子和细胞表面磷脂酰丝氨酸的同时监测
    作者:Yoshitaka Furuta and Zheng Zhou日期:10/20/2021,浏览量:116,Q&A: 0
    [Abstract]

    Calcium ions trigger many cellular events, including the release of neurotransmitters at the synaptic terminal and excitotoxic cell death. Recently, we have discovered that a transient increase in the level of cytoplasmic Ca2+ triggers the exposure of phosphatidylserine (PS) on the surfaces of necrotic cells in the nematode Caenorhabditis elegans.

    ...
    Isolation and Electrophysiology of Murine Sympathetic Postganglionic Neurons in the Thoracic Paravertebral Ganglia
    小鼠胸椎旁神经节交感神经节后神经元的分离与电生理研究
    作者:Mallika Halder, Michael Lee McKinnon, Yaqing Li, Peter Wenner and Shawn Hochman日期:10/20/2021,浏览量:101,Q&A: 0
    [Abstract]

    The thoracic paravertebral sympathetic chain postganglionic neurons (tSPNs) represent the predominant sympathetic control of vascular function in the trunk and upper extremities. tSPNs cluster to form ganglia linked by an interganglionic nerve and receive multisegmental convergent and divergent synaptic input from cholinergic sympathetic

    ...
    Analysis of Leukemia Cell Metabolism through Stable Isotope Tracing in Mice
    小鼠白血病细胞代谢的稳定同位素示踪分析
    作者:Nick van Gastel, Jessica B. Spinelli, Marcia C. Haigis and David T. Scadden日期:10/05/2021,浏览量:821,Q&A: 0
    [Abstract]

    Once thought to be a mere consequence of the state of a cell, intermediary metabolism is now recognized as a key regulator of mammalian cell fate and function. In addition, cell metabolism is often disturbed in malignancies such as cancer, and targeting metabolic pathways can provide new therapeutic options. Cell metabolism is mostly studied in

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    Structural and Functional Mapping of Mesenchymal Bodies
    间充质体的结构和功能图谱
    作者:Sébastien Sart, Raphaël F.-X. Tomasi, Antoine Barizien, Gabriel Amselem, Ana Cumano and Charles N. Baroud日期:10/05/2021,浏览量:1218,Q&A: 0
    [Abstract]

    The formation of spheroids with mesenchymal stem/stromal cells (MSCs), mesenchymal bodies (MBs), is usually performed using bioreactors or conventional well plates. While these methods promote the formation of a large number of spheroids, they provide limited control over their structure or over the regulation of their environment. It has

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    Phalloidin Staining of Actin Filaments for Visualization of Muscle Fibers in Caenorhabditis elegans
    秀丽隐杆线虫肌动蛋白丝的Phalloidin染色用于肌纤维可视化
    作者:Mario Romani and Johan Auwerx日期:10/05/2021,浏览量:745,Q&A: 0
    [Abstract]

    Advances in C. elegans research have allowed scientists to recapitulate different human disorders, from neurodegenerative diseases to muscle dysfunction, in these nematodes. Concomitantly, the interest in visualizing organs affected by these conditions has grown, leading to the establishment of different antibody- and dye-based staining protocols

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    Isolation, Culture, and Identification of Primary Müller Cells from Human Retina
    人视网膜原代 Müller 细胞的分离、培养和鉴定
    作者:Yingying Chen, Ting Zhang, Shaoxue Zeng, Michelle Yam, Mark C. Gillies and Ling Zhu日期:10/05/2021,浏览量:448,Q&A: 0
    [Abstract]

    Müller cells, the major glial cells of the retina, play vital roles in maintaining redox homeostasis and retinal metabolism. An immortalized human Müller cell line (MIO-M1) is widely used as an in vitro model to study Müller cells’ function, but they may not be exactly the same as primarily cultured human Müller cells. The use of human

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    Calcium Imaging in T Lymphocytes: a Protocol for Use with Genetically Encoded or Chemical Ca2+ Indicators
    T 淋巴细胞中的钙成像:与遗传编码或化学Ca2+指示一起使用的实验方案
    作者:Amit Jairaman and Michael D. Cahalan日期:10/05/2021,浏览量:556,Q&A: 0
    [Abstract]

    Elevations in cytosolic calcium (Ca2+) drive a wide array of immune cell functions, including cytokine production, gene expression, and cell motility. Live-cell imaging of cells loaded with ratiometric chemical Ca2+ indicators remains the gold standard for visualization and quantification of intracellular Ca2+ signals; ratiometric imaging can be

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