细胞生物学

分类

    现刊
    Quantitative Analysis of Actin Cable Length in Yeast
    酵母中肌动蛋白索长度的定量分析
    作者:Shane G. McInally, Jane Kondev and Bruce L. Goode日期:05/05/2022,浏览量:677,Q&A: 0

    Polarized actin cables in S. cerevisiae are linear bundles of crosslinked actin filaments that are assembled by two formins, Bnr1 (localized to the bud neck), and Bni1 (localized to the bud tip). Actin is polymerized at these two sites, which

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    Immunoisolation of Endosomal Recycling Vesicles from Saccharomyces cerevisiae
    酿酒酵母内体循环囊泡的免疫分离
    作者:Sho W. Suzuki and Scott D. Emr日期:05/05/2022,浏览量:412,Q&A: 0

    Endosomal recycling is essential for the appropriate function of the endosome. During this process, endosomal coat complexes (i.e., retromer, and Mvp1) are recruited to the endosome, and deform its membrane to form recycling vesicles. To further

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    Ex vivo Human Skin Infection with Herpes Simplex Virus 1
    人皮肤单纯疱疹病毒1的体外感染
    作者:Nydia C. De La Cruz, Maureen Möckel, Lisa Wirtz and Dagmar Knebel-Mörsdorf日期:05/05/2022,浏览量:307,Q&A: 0

    Although herpes simplex virus 1 (HSV-1) is a well-studied virus, how the virus invades its human host via skin and mucosa to reach its receptors and initiate infection remains an open question. For studies of HSV-1 infection in skin, mice have been

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    Immunoisolation of Endosomal Recycling Vesicles from Saccharomyces cerevisiae
    酿酒酵母内体循环囊泡的免疫分离
    作者:Sho W. Suzuki and Scott D. Emr日期:05/05/2022,浏览量:412,Q&A: 0
    [Abstract]

    Endosomal recycling is essential for the appropriate function of the endosome. During this process, endosomal coat complexes (i.e., retromer, and Mvp1) are recruited to the endosome, and deform its membrane to form recycling vesicles. To further analyze this, we developed a protocol for the immunoisolation of recycling vesicles from budding yeast.

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    Quantitative Analysis of Actin Cable Length in Yeast
    酵母中肌动蛋白索长度的定量分析
    作者:Shane G. McInally, Jane Kondev and Bruce L. Goode日期:05/05/2022,浏览量:677,Q&A: 0
    [Abstract]

    Polarized actin cables in S. cerevisiae are linear bundles of crosslinked actin filaments that are assembled by two formins, Bnr1 (localized to the bud neck), and Bni1 (localized to the bud tip). Actin is polymerized at these two sites, which results in cables extending along the cell cortex toward the back of the mother cell. These cables serve

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    Ex vivo Human Skin Infection with Herpes Simplex Virus 1
    人皮肤单纯疱疹病毒1的体外感染
    作者:Nydia C. De La Cruz, Maureen Möckel, Lisa Wirtz and Dagmar Knebel-Mörsdorf日期:05/05/2022,浏览量:307,Q&A: 0
    [Abstract]

    Although herpes simplex virus 1 (HSV-1) is a well-studied virus, how the virus invades its human host via skin and mucosa to reach its receptors and initiate infection remains an open question. For studies of HSV-1 infection in skin, mice have been used as animal models. Murine skin infection can be induced after injection or scratching of the

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    Measurement of Cell Intrinsic TGF-β Activation Mediated by the Integrin αvβ8
    整合素αvβ8介导的细胞固有TGF-β活化测定
    作者:Robert Ian Seed and Stephen Lloyd Nishimura日期:04/20/2022,浏览量:1062,Q&A: 0
    [Abstract]

    Transforming growth factor beta (TGF-β) is a multi-functional cytokine that plays a significant role in multiple diseases, including fibrosis and tumor progression. Whilst the biologic effects of TGF-β are well characterized, it is unclear how TGF-β signaling is regulated to impart specific responses within certain cell types. One

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    Isolation of tdTomato Expressing Inter-follicular Epidermal Melanocytes or Keratinocytes from Mouse Tail Skin
    从小鼠尾部皮肤中分离tdTomato表达滤泡间表皮黑色素细胞或角质形成细胞
    作者:Samuel Pop, Oscar Urtatiz and Catherine D. Van Raamsdonk日期:04/20/2022,浏览量:931,Q&A: 0
    [Abstract]

    The epidermis is the outermost layer of the skin. It is made up of mostly keratinocytes along with a small number of melanocytes and Langerhans cells. Melanocytes produce a pigment called melanin, which is transferred to the keratinocytes, and protects these cells from damage from UV radiation, as well as generating hair and skin colours. In this

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    Production of Recombinant Hepatitis B virus (HBV) and Detection of HBV in Infected Human Liver Organoids
    重组乙型肝炎病毒 (HBV) 的产生和受感染的人类肝脏类器官中 HBV 的检测
    作者:Tanvir Hossain, Shahla Romal and Tokameh Mahmoudi日期:04/20/2022,浏览量:383,Q&A: 0
    [Abstract]

    The absence of long term, primary untransformed in vitro models that support hepatitis B virus (HBV) infection and replication have hampered HBV pre-clinical research, which was reflected in the absence of a curative therapy until recently. One of the limitations for in vitro HBV research has been the absence of high titer and pure recombinant HBV

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    Visualization and Purification of Caenorhabditis elegans Germ Granule Proteins Using Proximity Labeling
    利用近似标记可视化和纯化秀丽隐杆线虫的胚芽颗粒蛋白
    作者:Hannah L. Hertz, Ian F. Price and Wen Tang日期:04/20/2022,浏览量:1023,Q&A: 0
    [Abstract]

    Membraneless organelles, such as germ granules and stress granules, are liquid-like condensates formed by phase transition. Recently, we and others have adopted proximity-based labeling methods to determine the composition of these membraneless compartments. Here, we describe the use of TurboID—an engineered promiscuous biotin

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    circFL-seq, A Full-length circRNA Sequencing Method
    circFL-seq,一种全长circRNA的测序方法
    作者:Zelin Liu and Ence Yang日期:04/20/2022,浏览量:757,Q&A: 1
    [Abstract]

    Due to overlapping sequences with linear cognates, identifying internal sequences of circular RNA (circRNA) remains a challenge. Recently, we have developed a full-length circRNA sequencing method (circFL-seq) and computational pipeline, to profile ordinary and fusion circRNA at the isoform level. Compared to short-read RNA-seq, rolling circular

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    High Throughput Blood-brain Barrier Organoid Generation and Assessment of Receptor-Mediated Antibody Transcytosis
    高通量血脑屏障类器官的产生和受体介导的抗体转胞吞作用的评估
    作者:Elena Kassianidou, Claire Simonneau, Alina Gavrilov and Roberto Villaseñor日期:04/20/2022,浏览量:509,Q&A: 0
    [Abstract]

    Targeting receptor-mediated transcytosis (RMT) is a successful strategy for drug delivery of biologic agents across the blood-brain barrier (BBB). The recent development of human BBB organoid models is a major advancement to help characterize the mechanisms of RMT and thus accelerate the design of brain delivery technologies. BBB organoids exhibit

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    Low-viscosity Matrix Suspension Culture for Human Colorectal Epithelial Organoids and Tumoroids
    用于人结直肠上皮类器官和类肿瘤的低粘度基质悬浮培养
    作者:Tao Tan, Yumiko Hirokawa, Jordan Clarke, Anuratha Sakthianandeswaren and Oliver M. Sieber日期:04/20/2022,浏览量:803,Q&A: 0
    [Abstract]

    Three-dimensional culture of human normal colorectal epithelium and cancer tissue as organoids and tumoroids has transformed the study of diseases of the large intestine. A widely used strategy for generating patient-derived colorectal organoids and tumoroids involves embedding cells in domes of extracellular matrix (ECM). Despite its success,

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